Noninvasive Visualization of Coronary Artery Endothelial Function in Healthy Subjects and in Patients With Coronary Artery Disease

Hays, Allison G.; Hirsch, Glenn A.; Kelle, Sebastian; Gerstenblith, Gary; Weiss, Robert G.; Stuber, Matthias

doi:10.1016/j.jacc.2010.06.036

Cited by 113 publications

(221 citation statements)

References 45 publications

Supporting

Mentioning

196

Contrasting

Order By: Relevance

“…Only recently has MRI been used to assess coronary endothelial vasomotion in subjects with coronary artery disease. [27][28][29] In the present study, we showed that endothelial damage was accompanied by an impaired endothelial-dependent vasodilation in response to acetylcholine. Both the atherosclerotic ApoE Ϫ/Ϫ mice and WT animals injected with RVV showed vasoconstriction compared with uninjected WT animals that showed vasodilation.…”

supporting

confidence: 59%

“…[22][23][24][25][26] Recently, MRI has also been used to assess endothelial function. [27][28][29] Gadofosveset is a clinically approved gadolinium-based contrast agent that reversibly binds to serum albumin, resulting in a prolonged vascular presence and a 5-to 10-fold increase in relaxivity (r 1 ). 30,31 Gadofosveset is predominantly present within the vessel lumen but may enter the vessel wall through leaky neovessels 32,33 and mechanically damaged endothelium.…”

Section: Clinical Perspective On P 719mentioning

confidence: 99%

See 1 more Smart Citation

Noninvasive Magnetic Resonance Imaging Evaluation of Endothelial Permeability in Murine Atherosclerosis Using an Albumin-Binding Contrast Agent

et al. 2012

View full text Add to dashboard Cite

Background-Endothelial dysfunction promotes atherosclerosis and precedes acute cardiovascular events. We investigated whether in vivo magnetic resonance imaging with the use of an albumin-binding contrast agent, gadofosveset, could detect endothelial damage associated with atherosclerosis in apolipoprotein E-deficient (ApoE Ϫ/Ϫ ) mice. Furthermore, we tested whether magnetic resonance imaging could noninvasively assess endothelial function by measuring the endothelial-dependent vasodilation in response to acetylcholine. Methods and Results-ApoEϪ/Ϫ mice were imaged at 4, 8, and 12 weeks after commencement of a high-fat diet. Statin-treated ApoE Ϫ/Ϫ mice were scanned after 12 weeks of a high-fat diet. Wild-type mice were imaged before and 48 hours after injection of Russell's viper venom, an endothelial toxin. Delayed enhancement magnetic resonance imaging and T1 mapping of the brachiocephalic artery, 30 minutes after injection of gadofosveset, showed increased vessel wall enhancement and relaxation rate (R 1 ) with progression of atherosclerosis in ApoE (R 1 ϭ3.0Ϯ0.65) mice showed less enhancement. Uptake of gadofosveset correlated with Evans blue staining, morphological changes of endothelial cells, and widening of the cell-cell junctions, suggesting that uptake occurs in regions of increased vascular permeability. Endothelial-dependent vasomotor responses showed vasoconstriction of the arteries of the ApoE Ϫ/Ϫ (Ϫ22.22Ϯ7.95%) and Russell's viper venom-injected (Ϫ10.37Ϯ17.60%) mice compared with wild-type mice (32.45Ϯ12.35%). Statin treatment improved endothelium morphology and function (Ϫ8.12Ϯ8.22%). Conclusions-We demonstrate the noninvasive assessment of endothelial permeability and function with the use of an albumin-binding magnetic resonance contrast agent. Blood albumin leakage could be a surrogate marker for the in vivo evaluation of interventions that aim to restore the endothelium. (Circulation. 2012;126:707-719.)Key Words: atherosclerosis Ⅲ endothelial dysfunction Ⅲ gadofosveset Ⅲ magnetic resonance imaging Ⅲ permeability A therosclerosis is a chronic disease of the vessels and a major cause of death in Western societies. Dysfunction of the vascular endothelium triggers leukocyte transmigration, platelet activation, smooth muscle cell proliferation, and vasoconstriction, which collectively promote the development of atherosclerosis. 1 Additionally, damaged endothelium can precipitate the complications of atherosclerosis through vasospasm and thrombosis, causing life-threatening cardiovascular events. Clinical Perspective on p 719Transport across the normal endothelium occurs between endothelial cells (ECs) (intercellular pathway) and/or through the ECs (transcytosis). Intercellular junctions with a diameter of Ϸ2 nm allow transport of small water-soluble molecules up to that diameter, 2 whereas breaks in the tight junctions with a diameter of Ϸ20 nm 3,4 accommodate the influx of albumin (diameter of Ϸ6 nm) and Evans blue dye (EBD). At the molecular level, oxidized low-density lipoprotein (1) decreases...

show abstract

supporting

confidence: 59%

Section: Clinical Perspective On P 719mentioning

confidence: 99%

Noninvasive Magnetic Resonance Imaging Evaluation of Endothelial Permeability in Murine Atherosclerosis Using an Albumin-Binding Contrast Agent

et al. 2012

View full text Add to dashboard Cite

show abstract

“…[15][16][17][18][19][20] Providing reliable techniques to identify atherosclerotic lesions in the early stages, however, is still challenging. In this study, we could show that alterations of local vascular elasticity precede the formation of atherosclerotic lesions.…”

Section: Discussionmentioning

confidence: 99%

“…Although MRI has recently been used to quantify impaired vascular elasticity via global PWV, 24 vascular distensibility, 25 and endothelial function 18,20 in humans, those studies were limited to patients with morphologically manifest atherosclerosis. PWV and vascular distensibility are parameters describing the arterial stiffness, which is primarily determined by the extracellular matrix, whereas the endothelial function measures the ability of the endothelium to regulate vascular tone in response to a variety of stimuli such as acetylcholine or physical exercise.…”

Section: Discussionmentioning

confidence: 99%

Local Arterial Stiffening Assessed by MRI Precedes Atherosclerotic Plaque Formation

Gotschy

Bauer

Schrodt

et al. 2013

Circ: Cardiovascular Imaging

View full text Add to dashboard Cite

) mice, which spontaneously develop atherosclerotic lesions of morphology similar to those observed in humans. 11,12 Local PWV and vessel wall thickness were evaluated by timeresolved flow and morphology measurement using ultrahighfield MR microscopy at 17.6 T. In addition, we performed histological examinations to investigate structural changes of the vessel wall at the time of imaging.Background-Atherosclerosis is known to impair vascular function and cause vascular stiffening. The aim of this study was to evaluate the potential predictive role of vascular stiffening in the early detection of atherosclerosis. Therefore, we investigated the time course of early functional and morphological alterations of the vessel wall in a murine atherosclerosis model. Because initial lesions are distributed inhomogeneously in early-stage atherosclerosis, MR microscopy was performed to measure vascular elasticity locally, specifically the local pulse wave velocity and the arterial wall thickness. Methods and Results-Local pulse wave velocity and the mean arterial wall thickness were determined in the ascending and the abdominal aortae of ApoE −/− and wild-type mice. In vivo MRI revealed that baseline pulse wave velocity and morphology were similar in 6-week-old ApoE −/− and WT mice, whereas at the age of 18 weeks, local pulse wave velocity was significantly elevated in ApoE −/− mice. Significantly increased vessel wall thickness was not found in ApoE −/− mice until the age of 30 weeks. Histological analysis of the aortae of ApoE −/− and WT mice showed that increased pulse wave velocity coincided with the fragmentation of the elastic laminae in the arterial wall, which is hypothesized to induce early vascular stiffening and may be promoted by macrophage-mediated matrix degradation. Conclusions-We

show abstract

“…Technological advances in MRI, image processing software, and development of molecular imaging agents have improved the characterization and measurement of most of these parameters and provide a promising tool for the noninvasive assessment of atherosclerotic disease. MRI sequences that allow velocity mapping of the coronary arteries 46,47 are now available and could be used for the calculation of ESS in the coronary tree.…”

Section: Discussionmentioning

confidence: 99%

Regions of Low Endothelial Shear Stress Colocalize With Positive Vascular Remodeling and Atherosclerotic Plaque Disruption

Phinikaridou

Hua

Pham

et al. 2013

Circ: Cardiovascular Imaging

View full text Add to dashboard Cite

2; P<0.001) compared with nondisrupted plaques. The peak ESS negatively correlated with the plaque area (r=−0.56, P<0.001) and remodeling ratio (r=−0.4, P=0.008). There was also a negative correlation between the mean ESS and the remodeling ratio (r=−0.55, P<0.001). Both the peak ESS and the mean ESS did not correlate with the % stenosis; there was a weak but statistically significant correlation with the % cross-sectional narrowing (r=0.3, P=0.002 and r=0.2, P=0.04, respectively). Receiver operating characteristic analysis showed that both mean (Area under the curve =0.78; 95% CI, 0.69-0.87) and peak ESS (Area under the curve=0.85; 95% CI, 0.78-0.93) identified disrupted plaques. Conclusions-We demonstrated that low ESS is associated with plaque burden, positive vascular remodeling, and plaque disruption in a rabbit model. Assessment of ESS by noninvasive MRI might be useful for assessing atherosclerotic risk. (Circ Cardiovasc Imaging. 2013;6:302-310.)

show abstract

Noninvasive Visualization of Coronary Artery Endothelial Function in Healthy Subjects and in Patients With Coronary Artery Disease

Cited by 113 publications

References 45 publications

Noninvasive Magnetic Resonance Imaging Evaluation of Endothelial Permeability in Murine Atherosclerosis Using an Albumin-Binding Contrast Agent

Noninvasive Magnetic Resonance Imaging Evaluation of Endothelial Permeability in Murine Atherosclerosis Using an Albumin-Binding Contrast Agent

Local Arterial Stiffening Assessed by MRI Precedes Atherosclerotic Plaque Formation

Regions of Low Endothelial Shear Stress Colocalize With Positive Vascular Remodeling and Atherosclerotic Plaque Disruption

Contact Info

Product

Resources

About