This paper proposes a multifractal least squares support vector machine detrended fluctuation analysis (MF-LSSVM-DFA) model. The system is an extension of the traditional MF-DFA model. To address potential overfitting or underfitting caused by the fixed-order polynomial fitting in MF-DFA, LSSVM is employed as a superior alternative for fitting. This approach enhances model accuracy and adaptability, ensuring more reliable analysis results. We utilize the p model to construct a multiplicative cascade time series to evaluate the performance of MF-LSSVM-DFA, MF-DFA, and two other models that improve upon MF-DFA from recent studies. The results demonstrate that our proposed modified model yields generalized Hurst exponents h(q) and scaling exponents τ(q) that align more closely with the analytical solutions, indicating superior correction effectiveness. In addition, we explore the sensitivity of MF-LSSVM-DFA to the overlapping window size s. We find that the sensitivity of our proposed model is less than that of MF-DFA. We find that when s exceeds the limited range of the traditional MF-DFA, h(q) and τ(q) are closer than those obtained in MF-DFA when s is in a limited range. Meanwhile, we analyze the performances of the fitting of the two models and the results imply that MF-LSSVM-DFA achieves a better outstanding performance. In addition, we put the proposed MF-LSSVM-DFA into practice for applications in the medical field, and we found that MF-LSSVM-DFA improves the accuracy of ECG signal classification and the stability and robustness of the algorithm compared with MF-DFA. Finally, numerous image segmentation experiments are adopted to verify the effectiveness and robustness of our proposed method.