Nonlinear Elastic and Inelastic Properties of Cells

Jung, Wonyeong; Li, Jing; Chaudhuri, Ovijit; Kim, Taeyoon

doi:10.1115/1.4046863

Cited by 18 publications

(6 citation statements)

References 182 publications

(246 reference statements)

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“…The stiffness of a cell is primarily maintained by the nucleus and the cytoskeleton, the latter of which is composed of three types of micro‐filaments, actin filaments, intermediate filaments, and microtubules. [ 8 ] Depending on the type of forces generated in and applied to the cell (extracellular tensile, intracellular tensile, or extracellular compressive), the mode of force transmission is different. In particular, different types of cells can use distinct compositions of the cytoskeletal networks to adapt and respond to external cues uniquely; yet how this is done in a complex multicellular tissue remains mysterious.…”

Section: Discussion and Summarymentioning

confidence: 99%

“…The mechanical properties of the cells are measured and quantified with multiple experimental methods. For instance, cell mechanics can be studied via various methods [ 88,89 ] such as micropipette aspiration, micro‐force sensors, cell pokers, optical tweezers, magnetic tweezers, [ 4,8 ] and AFM. [ 4 ] AFM was also used to measure adhesion forces of single cells from the zebrafish gastrula embryos to substrates with fibronectin, [ 91 ] and to measure the specific de‐adhesion forces which are generated to split single mesendodermal cells from substrates.…”

Section: Discussion and Summarymentioning

confidence: 99%

“…Single‐cell stiffness can be measured by multiple experimental approaches including micropipette aspiration, micro‐force sensors, cell pokers, optical tweezers, and magnetic tweezers. [ 4,8 ] Among these approaches, the most well‐developed technique is the atomic force microscopy (AFM) indentation assay. [ 4 ] It controls an elastic AFM cantilever tip to either directly or indirectly (by attaching a microsphere to increase the contact area) compress a single cell that is seeded onto a surface.…”

Section: Generation Of Mechanical Forces In Cells and Tissuesmentioning

confidence: 99%

“…The stiffness of a cell is primarily maintained by the nucleus and the cytoskeleton, the latter of which consists of three types of biopolymers: actin filament, intermediate filament, and microtubule. [ 8 ] Together with the cell membrane (including the cell cortex), these compose the main subcellular structures that allow force transmission on and inside a cell. Depending on the type of forces generated in and applied to the cell, which can be either extracellular tensile, intracellular tensile or extracellular compressive, the mode of force transmission is different.…”

Section: Generation Of Mechanical Forces In Cells and Tissuesmentioning

confidence: 99%

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Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis

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Xiu

Chen

et al. 2021

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Embryonic morphogenesis is a biological process which depicts shape forming of tissues and organs during development. Unveiling the roles of mechanical forces generated, transmitted, and regulated in cells and tissues through these processes is key to understanding the biophysical mechanisms governing morphogenesis. To this end, it is imperative to measure, simulate, and predict the regulation and control of these mechanical forces during morphogenesis. This article aims to provide a comprehensive review of the recent advances on mechanical properties of cells and tissues, generation of mechanical forces in cells and tissues, the transmission processes of these generated forces during cells and tissues, the tools and methods used to measure and predict these mechanical forces in vivo, in vitro, or in silico, and to better understand the corresponding regulation and control of generated forces. Understanding the biomechanics and mechanobiology of morphogenesis will not only shed light on the fundamental physical mechanisms underlying these concerted biological processes during normal development, but also uncover new information that will benefit biomedical research in preventing and treating congenital defects or tissue engineering and regeneration.

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Section: Discussion and Summarymentioning

confidence: 99%

Section: Discussion and Summarymentioning

confidence: 99%

Section: Generation Of Mechanical Forces In Cells and Tissuesmentioning

confidence: 99%

Section: Generation Of Mechanical Forces In Cells and Tissuesmentioning

confidence: 99%

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Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis

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Xiu

Chen

et al. 2021

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“…Клетки обладают широким спектром нелинейно-упругих и неупругих свойств, которые определяются механическими характеристиками клеточных компонентов [11]. Биомеханические свойства мембраны нейтрофилов (Нф) важны при морфогенезе, цитокинезе и миграции, и влияют на их способность к проникновению через стенку сосуда, что определяет их роль в канцерогенезе [4].…”

Section: Introductionunclassified

Influence of proinflammatory cytokines on membrane rigidity and morphofunctional state of circulating neutrophils in ovarian tumors

Абакумова

Генинг

et al. 2022

Med. immunol.

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Priming and activation mediated by cytokines cause transient reactions of actin polymerization in neutrophils (Nph), expansion and softening of cells, changes in receptor status, phagocytic ability, and generation of extracellular traps (NET), thus ultimately determining pro- or antitumor phenotype of Nph. To assess the effects of pro-inflammatory cytokines on membrane rigidity and morphofunctional state of neutrophils in benign tumors and ovarian cancer, the levels of circulating cytokines (IL-2, IL-18, MCP-1, TIMP-1), expression of adhesion markers (CD11b), degranulation (CD63), as well as FcãRIIIb receptors promoting phagocytosis (CD16). Ability of neutrophils to phagocytosis and the formation of NET was determined. Statistical evaluation of the data obtained was carried out using Statistica 13.0, Jamovi 1.6.5.0 software. An increase in membrane rigidity was found in benign and malignant ovarian tumors. In benign ovarian tumors, phagocytic activity and expression of CD11b were enhanced. In ovarian cancer, the number of CD11b+Nph and CD63+Nph were increased. Meaanwhile, at initial cancer stage, the ability to form NET predominates, and phagocytic activity increases with advancing ovarian cancer. Serum MCP-1 levels are elevated in benign tumors and at all stages of ovarian cancer. IL-2 is elevated at early stage and in advanced ovarian cancer. The level of IL-18 and TIMP-1 in the serum of patients with benign ovarian tumor did not differ significantly from the norm. Using multiple regression approach, the dependence of neutrophil membrane rigidity on the levels of circulating IL-2, TIMP-1, MCP-1, IL-18 was revealed in benign ovarian tumors, and a direct correlation was found between the neutrophil membrane rigidity and CD11b expression. Only IL-2 was associated with neutrophil membrane rigidity in ovarian cancer. At the same time, the rigidity of the neutrophil membrane directly correlated with CD16, CD63, expression like as with phagocytic index and inversely correlates with the number of traps. A combination of IL-2, MCP-1 and membrane rigidity of circulating neutrophils (based on multivariate analysis) could be used for differential diagnosis of ovarian cancer. Thus, in a benign ovarian tumor, circulating proinflammatory cytokines are associated with increased rigidity of neutrophil membrane and increase in their adhesion capacity. In ovarian cancer, only IL-2 is associated with altered rigidity of circulating neutrophils. Increase of the latter index is accompanied by elevated phagocytic activity and decreased ability to form NET.

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Characterising the Mechanism of Cell Failure Due to Shear Using a Combined Numerical and Analytical Approach

Smart,

Hooker,

Bhattacharjee

et al. 2024

Mechanisms and Machine Science

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Nonlinear Elastic and Inelastic Properties of Cells

Cited by 18 publications

References 182 publications

Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis

Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis

Influence of proinflammatory cytokines on membrane rigidity and morphofunctional state of circulating neutrophils in ovarian tumors

Characterising the Mechanism of Cell Failure Due to Shear Using a Combined Numerical and Analytical Approach

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