Nonlinear Impedance of Whole Cells Near an Electrode as a Probe of Mitochondrial Activity

Palanisami, Akilan; Mercier, George T.; Fang, Jie; Miller, John H.

doi:10.3390/bios1020046

Cited by 9 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…observed the generation of harmonics from living yeast and argued that it was caused by an enzyme present in the membrane [13][14][15] . A nonlinearity generated near the electrode has been reported 10,12,16 . The generation of the nonlinearity depends on the shape of the electrode and is affected by the intensity and distortion of the electric field near the electrode 10 .…”

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 99%

Generation of nonlinearity in the electrical response of yeast suspensions

Muraji

Tamura

Tanaka

et al. 2021

Preprint

View full text Add to dashboard Cite

The mechanism through which nonlinearity is generated in the response waveform of the electric current obtained by applying alternating current voltage to yeast suspension has not yet been elucidated. In this paper, we showed that the response waveform depends on the applied voltage and frequency. The results showed that distortion (nonlinearity) in the waveform increases as the applied voltage increases and/or the frequency decreases. We suggest a model for the generation of nonlinearity based on the influx of potassium ions into the cell via potassium ion channels and transporters in the membrane due to the applied voltage. Furthermore, we validated this model by simulating an electrical circuit.

show abstract

Section: Cells Within Living Organisms Transmit Information Through Electrical Signals and Chemical Reactions 12 Characterizing The Elecmentioning

confidence: 99%

Generation of nonlinearity in the electrical response of yeast suspensions

Muraji

Tamura

Tanaka

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Saccharomyces cerevisiae ( S. cerevisiae ) shares the complex internal cell structure of animal cells and serves as an ideal model for conducting research in higher eukaryotes. S. cerevisiae has been used extensively to study cellular mechanisms, including DNA damage and repair as well as systematic fungal infections [4,5,6]. Evidence from previous findings indicate that there are several membrane redox centers in most eukaryotic cells that can be targeted to monitor redox activities in the presence of certain drugs [7,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…We therefore intend to develop a simple procedure based on electrochemistry that allows simple and rapid screening of membrane-targeted leads. The concept is based on the premises that membrane modulation of a ligand can offset the chemical balance, thereby enhancing the flow of ions/charges and potentiating the activities of antimicrobial compounds [6,13]. To test these hypotheses, Dioclea reflexa seed extracts and three extensively studied antifungal and antibiotic drugs were selected to investigate their electrochemical behaviors using S. cerevisiae cells as a model biological system.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs

et al. 2019

View full text Add to dashboard Cite

Dioclea reflexa bioactive compounds have been shown to contain antioxidant properties. The extracts from the same plant are used in traditional medical practices to treat various diseases with impressive outcomes. In this study, ionic mobility in Saccharomyces cerevisiae cells in the presence of D. reflexa seed extracts was monitored using electrochemical detection methods to link cell death to ionic imbalance. Cells treated with ethanol, methanol, and water extracts were studied using cyclic voltammetry and cell counting to correlate electrochemical behavior and cell viability, respectively. The results were compared with cells treated with pore-forming Amphotericin b (Amp b), as well as Fluconazole (Flu) and the antimicrobial drug Rifampicin (Rif). The D. reflexa seed water extract (SWE) revealed higher anodic peak current with 58% cell death. Seed methanol extract (SME) and seed ethanol extract (SEE) recorded 31% and 22% cell death, respectively. Among the three control drugs, Flu revealed the highest cell death of about 64%, whereas Amp b and Rif exhibited cell deaths of 35% and 16%, respectively, after 8 h of cell growth. It was observed that similar to SWE, there was an increase in the anodic peak current in the presence of different concentrations of Amp b, which also correlated with enhanced cell death. It was concluded from this observation that Amp b and SWE might follow similar mechanisms to inhibit cell growth. Thus, the individual bioactive compounds from the water extracts of D. reflexa seeds could further be purified and tested to validate their potential therapeutic application. The strategy to link electrochemical behavior to biochemical responses could be a simple, fast, and robust screening technique for new drug targets and to understand the mechanism of action of such drugs against disease models.

show abstract

“…At lower frequencies, due to the high resistance of cells, current passes primarily through the fluids and not through the cell membranes [5]. At higher frequencies, the electrical signals can penetrate through the cell membranes.…”

Section: Introductionmentioning

confidence: 99%

Reconfigurable, Multi-channel and Modular Bioimpedance Spectroscopy System on Field Programmable Gate Arrays

Nordin¹,

Al-Hashimi²,

Azman³

2017

Preprint

View full text Add to dashboard Cite

This paper presents the design and implementation of a multichannel bio-impedance spectroscopy system on field programmable gate arrays (FPGA). The proposed system is capable of acquiring multiple signals from multiple bio-impedance sensors, process the data on the FPGA and store the final data in the on-board Memory. The system employs the Digital Automatic Balance Bridge (DABB) method to acquire data from biosensors. The DABB measures initial data of a known impedance to extrapolate the value of the impedance for the device under test. This method offers a simpler design because the balancing of the circuit is done digitally in the FPGA rather than using an external circuit. Calculations of the impedance values for the device under test were done in the processor. The final data is sent to an onboard Flash Memory to be stored for later access. The control unit handles the interfacing and the scheduling between these different modules (Processor, Flash Memory) as well as interfacing to multiple Balance Bridge and multiple biosensors. The system has been simulated successfully and has comparable performance to other FPGA based solutions. The system has a robust design that is capable of handling and interfacing input from multiple biosensors. Data processing and storage is also performed with minimal resources on the FPGA.

show abstract

Nonlinear Impedance of Whole Cells Near an Electrode as a Probe of Mitochondrial Activity

Cited by 9 publications

References 31 publications

Generation of nonlinearity in the electrical response of yeast suspensions

Generation of nonlinearity in the electrical response of yeast suspensions

Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs

Reconfigurable, Multi-channel and Modular Bioimpedance Spectroscopy System on Field Programmable Gate Arrays

Contact Info

Product

Resources

About