Nonlinear Optical Properties of Proteins Measured by Hyper-Rayleigh Scattering in Solution

Clays, Koen; Hendrickx, Eric; Triest, Myriam; Verbiest, Thierry; Persoons, André; Dehu, C.; Brédas, J. L.

doi:10.1126/science.262.5138.1419

Cited by 152 publications

(99 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This behaviour is dominated by features associated with a two-level optical response but, provided the pump or harmonic frequencies are close to resonance, broadly similar effects are anticipated in systems of considerably greater electronic complexity. In this connection, there are also potential applications in monitoring processes of molecular self-assembly, developing principles which have been established for this purpose on the basis of incoherent (hyper-Rayleigh) second harmonic scattering [32,33]. The characteristic signal recovery which we have identified is the key feature, and determination of the biexponential form of the harmonic profile will faithfully register the dynamics of excited state decay.…”

Section: Discussionmentioning

confidence: 99%

Local coherence and the temporal development of second harmonic emission

Andrews¹,

Romero²

2001

J. Phys. B: At. Mol. Opt. Phys.

View full text Add to dashboard Cite

In a variety of mesoscopically disordered systems, high levels of optical excitation resulting from pulsed laser irradiation can establish optical coherence within separate particles or locally ordered domains, leading to second harmonic emission whose temporal signature characterizes the decay of the excited state population. Examples of such systems will include colloids, cell and membrane suspensions, and many plastics, glasses and other modern materials. With pulsed excitation of sufficient intensity to elicit the onset of saturation, second harmonic emission on the throughput of a subsequent probe beam exhibits a characteristic decay and recovery. Detailed calculations show that such features arise not only in systems whose optical response involves two discrete levels, but also in systems of considerably greater electronic complexity. Deconvolution of the temporal trace of the harmonic signal serves as an independent means of monitoring the decay of the excited state. The extent of recovery in the harmonic signal also serves to register the extent of local coherence, and hence in many systems the localization of structural order. Finally, the principles introduced in the theory are shown to be applicable to other types of system such as certain photochromic materials.

show abstract

Section: Discussionmentioning

confidence: 99%

Local coherence and the temporal development of second harmonic emission

Andrews¹,

Romero²

2001

J. Phys. B: At. Mol. Opt. Phys.

View full text Add to dashboard Cite

show abstract

“…One approach is based on the suggestion that a large light-induced redistribution of electronic charge in the vertically excited state causes alterations in the surrounding protein matrix and leads to the subsequent protein structural transformations (3). In support of this mechanism, there is unequivocal evidence based on nonlinear optics data (6)(7)(8) that, with similarity to retinals in solution (9), light excitation of bR does indeed initiate a large light-induced electron redistribution in the polyene. However, the connection between this charge redistribution and protein conformational changes has remained elusive.…”

mentioning

confidence: 99%

Microsecond atomic force sensing of protein conformational dynamics: Implications for the primary light-induced events in bacteriorhodopsin

Rousso

Khachatryan²,

Gat³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In this paper a new atomic force sensing technique is presented for dynamically probing conformational changes in proteins. The method is applied to the light-induced changes in the membrane-bound proton pump bacteriorhodopsin (bR). The microsecond time-resolution of the method, as presently implemented, covers many of the intermediates of the bR photocycle which is well characterized by spectroscopical methods. In addition to the native pigment, we have studied bR proteins substituted with chemically modified retinal chromophores. These synthetic chromophores were designed to restrict their ability to isomerize, while maintaining the basic characteristic of a large lightinduced charge redistribution in the vertically excited Franck--Condon state. An analysis of the atomic force sensing signals lead us to conclude that protein conformational changes in bR can be initiated as a result of a light-triggered redistribution of electronic charge in the retinal chromophore, even when isomerization cannot take place. Although the coupling mechanism of such changes to the light-induced proton pump is still not established, our data question the current working hypothesis which attributes all primary events in retinal proteins to an initial transNcis isomerization.

show abstract

“…The centre of symmetry is necessarily broken when preferred helicity is achieved in supramolecular organization. Polymers of such organized geometry are very interesting since they can supplement the directional order of donor-acceptor p-electron system and the dipole units are tilted in one direction along the polymer axis which leads to high second harmonic generation ability [1][2][3][4]. Optically pure materials which have only left handed/right handed symmetry are inherently non-centrosymmetric [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Isosorbide based chiral polyurethanes: optical and thermal studies

Varkey

Sreekumar

2010

J Mater Sci

View full text Add to dashboard Cite

A new class of chiral polyurethanes containing amido linkages in the polymer backbone have been synthesized by reacting toluene diisocyanate with isosorbide (IS) chiral moiety and the chromophores [N,N 0 -ethane-1,2-diyl bis(6-hydroxy hexanamide), N,N 0 -butane-1,4-diyl bis(6-hydroxy hexanamide) and N,N 0 -hexane-1,6-diyl bis (6-hydroxy hexanamide)]. The corresponding chromophores were obtained by the aminolysis of e-caprolactone by using the diamines, diaminoethane, diaminobutane and diaminohexane, respectively. All the polymers were synthesized according to the symmetry conditions so as to obtain the non-centrosymmetric environment. A series of polyurethanes were synthesized by varying the chiralchromophore composition. The polyurethanes developed were characterized by optical and thermal methods.

show abstract

Nonlinear Optical Properties of Proteins Measured by Hyper-Rayleigh Scattering in Solution

Cited by 152 publications

References 35 publications

Local coherence and the temporal development of second harmonic emission

Local coherence and the temporal development of second harmonic emission

Microsecond atomic force sensing of protein conformational dynamics: Implications for the primary light-induced events in bacteriorhodopsin

Isosorbide based chiral polyurethanes: optical and thermal studies

Contact Info

Product

Resources

About