In the present study, the shear failure mode and mechanical properties of the core structure of biomimetic fully integrated honeycomb plates with sealing edges were investigated experimentally and through the finite element method. The findings are as follows: (1) the failure mode of the sealing edges and honeycomb walls perpendicular to the shear direction is mainly debonding between the fiber and matrix, whereas fiber breakage, debonding between the fiber and matrix and exfoliation of the resin matrix occur in the sealing edges parallel to the shear direction. Meanwhile, the reasonableness and feasibility of the double shear testing apparatus designed in this study were verified, thus confirming the results of research are reliable and valid. (2) Shear failure of the core structure of fully integrated honeycomb plates is mainly fiber debonding appearing in the middle surface of the core structure, which is a failure of the material interface. Stripping failure in the joint interface of the core layer and upper and lower plates does not occur, which indicates that the biological structure possesses excellent integral mechanical properties. (3) The sealing edges parallel to the shear direction and the honeycomb walls that are oriented 30 degrees to the shear direction are the first to fail, followed by the sealing edges and honeycomb walls perpendicular to the shear direction, which is consistent with the microscopic failure phenomenon observed in both directions. To prevent failure at the material interface, the fully integrated honeycomb plates manufactured in this experiment require further improvements. Thus, countermeasures are proposed, such as pre-treating the fiber surface. These findings will specify future research directions to perfect fully integrated honeycomb plates and improve the shear mechanical properties of core structures.