Nonmetabolizable Arabinose Inhibits &lt;i&gt;Vibrio&lt;/i&gt; &lt;i&gt;cholerae&lt;/i&gt; Growth in M9 Medium with Gluconate as the Sole Carbon Source

Golder, Taniya; Mukhopadhyay, Asish K.; Koley, Hemanta; Nandy, Ranjan K.

doi:10.7883/yoken.jjid.2019.304

Cited by 2 publications

(4 citation statements)

References 40 publications

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“…It was recently reported that L-Ara inhibits the proliferation of V. cholerae [14]. Here, we show that it is associated with a change in the morphology of the cell from a curved rod shape to a spherical form (Figure 1).…”

Section: Discussionsupporting

confidence: 69%

“…Nevertheless, the E. coli PBAD system proved to be very effective in V. cholerae, which suggested that L-Ara was imported in the cytoplasm of the cells. However, we and others recently reported that L-Ara could interfere with the growth of the bacterium, calling for a better understanding of the impact of L-Ara on the physiology of V. cholerae and the pathway leading to its toxicity [14].…”

Section: Introductionmentioning

confidence: 97%

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L-arabinose induces the formation of viable non-proliferatingVibrio choleraespheroplasts

Espinosa

Daniel

Hernández

et al. 2020

Preprint

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A general survival strategy of many life forms faced with harmful growth conditions is to enter into a non-proliferating state until conditions suitable for growth are restored. In bacteria, this survival strategy is associated with antimicrobial tolerance, chronic infections and environmental dispersion. In particular, the agent of the deadly human disease cholera, Vibrio cholerae, undergoes a morphological transition from a rod-shaped proliferative form to a spherical non-proliferating form after exposure to cold or cell wall targeting antibiotics. Growth is resumed when the adverse conditions have ceased.Here, we show that a component of the hemicellulose and pectin of terrestrial plants, L-arabinose, triggers the formation of non-proliferating V. cholerae spherical cells, which are able to return to growth when L-arabinose is removed from the growth medium. We found that the cell wall of L-arabinose treated V. cholerae cells has a peptidoglycan composition similar to the cell wall of spheroplasts and that they revert to a wild-type morphology through the formation of branched cells like L-forms. Unlike L-forms, however, they are osmo-resistant. Through a random Tn genetic screen for mutants insensitive to L-arabinose, we identified genes involved in the uptake and catabolism of galactose and in glycolysis. We hypothesize that L-arabinose is enzymatically processed by the galactose catabolism and glycolysis pathways until it is transformed in a product that cannot be further recognized by V. cholerae enzymes. Accumulation of this enzymatic by-product triggers the formation of viable non-dividing cell wall deficient spherical cells.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 97%

L-arabinose induces the formation of viable non-proliferatingVibrio choleraespheroplasts

Espinosa

Daniel

Hernández

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…It was recently reported that L-Ara inhibited the proliferation of V. cholerae (15,16). Here, we show that it is associated with a change in the morphology of the cells from a curved rod shape to a spherical form (Figure 1).…”

Section: Discussionmentioning

confidence: 96%

“…Nevertheless, the E. coli P BAD system proved to be very effective in V. cholerae, which suggested that L-Ara was imported in the cytoplasm of the cells. However, we and others recently reported that L-Ara could interfere with the growth of V. cholerae (15,16), calling for a better understanding of the impact of L-Ara on the physiology of this bacterium.…”

Section: Introductionmentioning

confidence: 93%

l -Arabinose Induces the Formation of Viable Nonproliferating Spheroplasts in Vibrio cholerae

Espinosa

Daniel

Hernández

et al. 2021

Appl Environ Microbiol

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Vibrio cholerae, the agent of the deadly human disease cholera, propagates as a curved rod-shaped bacterium in warm waters. It is sensitive to cold, but persists in cold waters under the form of viable but non-dividing coccoidal shaped cells. Additionally, V. cholerae is able to form non-proliferating spherical cells in response to cell wall damage. It was recently reported that L-arabinose, a component of the hemicellulose and pectin of terrestrial plants, stops the growth of V. cholerae. Here, we show that L-arabinose induces the formation of spheroplasts that lose the ability to divide and stop growing in volume over time. However, they remain viable and upon removal of L-arabinose they start expanding in volume, form branched structures and give rise to cells with a normal morphology after a few divisions. We further show that WigKR, a histidine kinase/response regulator pair implicated in the induction of a high expression of cell wall synthetic genes, prevents the lysis of the spheroplasts during growth restart. Finally, we show that the physiological perturbations result from the import and catabolic processing of L-arabinose by the V. cholerae homolog of the E. coli galactose transport and catabolic system. Taken together, our results suggest that the formation of non-growing spherical cells is a common response of Vibrios exposed to detrimental conditions. They also permit to define conditions preventing any physiological perturbation of V. cholerae when using L-arabinose to induce gene expression from the tightly regulated promoter of the Escherichia coli araBAD operon.ImportanceVibrios among other bacteria form transient cell wall deficient forms as a response to different stresses and revert to proliferating rods when permissive conditions have been restored. Such cellular forms have been associated to antimicrobial tolerance, chronic infections and environmental dispersion.The effect of L-Ara on V. cholerae could provide an easily tractable model to study the ability of Vibrios to form viable reversible spheroplasts. Indeed, the quick transition to spheroplasts and reversion to proliferating rods by addition or removal of L-Ara is ideal to understand the genetic program governing this physiological state and the spatial rearrangements of the cellular machineries during cell shape transitions.

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Nonmetabolizable Arabinose Inhibits <i>Vibrio</i> <i>cholerae</i> Growth in M9 Medium with Gluconate as the Sole Carbon Source

Cited by 2 publications

References 40 publications

L-arabinose induces the formation of viable non-proliferatingVibrio choleraespheroplasts

L-arabinose induces the formation of viable non-proliferatingVibrio choleraespheroplasts

l -Arabinose Induces the Formation of Viable Nonproliferating Spheroplasts in Vibrio cholerae

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