Nonmucoid conversion of mucoid<i>Pseudomonas aeruginosa</i>induced by sulfate-stimulated growth

Min, Kyung Bae; Lee, Kang-Mu; Oh, Young Taek; Yoon, Sang Sun

doi:10.1111/1574-6968.12600

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…5E and Movie S7). Even though alginate did not seem to be directly involved in matrix thickening, alginate is known to be an important factor for virulence and antibiotic resistance in P. aeruginosa biofilms (33,34). Therefore, we evaluated alginate production in the mono-and dual-species biofilms via the alginate assay.…”

Section: Discussionmentioning

confidence: 99%

Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm

Lee

Kim

et al. 2017

Appl Environ Microbiol

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Biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ECMs). Clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, following the introduction of the first biofilm infection model by J. W. Costerton and colleagues (J. Lam, R. Chan, K. Lam, and J. W. Costerton, Infect Immun 28:546-556, 1980). However, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. and are two frequently identified bacterial species in biofilm infections; nevertheless, the interactions between these two species, especially during biofilm growth, are not clearly understood. In this study, we observed phenotypic changes in a dual-species biofilm of and, including a dramatic increase in biofilm matrix thickness. For clear elucidation of the spatial distribution of the dual-species biofilm, and were labeled with red and green fluorescence, respectively. was located at the lower part of the dual-species biofilm, while developed a structured biofilm on the upper part. Mutants with altered exopolysaccharide (EPS) productions were constructed in order to determine the molecular basis for the synergistic effect of the dual-species biofilm. Increased biofilm matrix thickness was associated with EPSs, not extracellular DNA. In particular, Pel and Psl contributed to interspecies and intraspecies interactions, respectively, in the dual-species and biofilm. Accordingly, targeting Pel and Psl might be an effective part of eradicating polymicrobial biofilms. Chronic infection is a serious problem in the medical field. Scientists have observed that chronic infections are closely associated with biofilms, and the vast majority of infection-causing biofilms are polymicrobial. Many studies have reported that microbes in polymicrobial biofilms interact with each other and that the bacterial interactions result in elevated virulence, in terms of factors, such as infectivity and antibiotic resistance. and are frequently isolated pathogens in chronic biofilm infections. Nevertheless, while both bacteria are known to be agents of numerous nosocomial infections and can cause serious diseases, interactions between the bacteria in biofilms have rarely been examined. In this investigation, we aimed to characterize and dual-species biofilms and to determine the molecular factors that cause synergistic effects, especially on the matrix thickening of the biofilm. We suspect that our findings will contribute to the development of more efficient methods for eradicating polymicrobial biofilm infections.

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Section: Discussionmentioning

confidence: 99%

Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm

Lee

Kim

et al. 2017

Appl Environ Microbiol

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“…Further analyses of the MS results identified mucB as a potential candidate. The mucABCD operon has been shown to influence P. aeruginosa virulence, mucoid conversion, and host persistence during chronic infections ( 27 ) ( Table S1A ). Analysis of mucB and steroid binding using SPR revealed no direct interaction ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa

Vidaillac

Yong

Aschtgen

et al. 2020

mBio

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Estrogen, a major female sex steroid hormone, has been shown to promote the selection of mucoid Pseudomonas aeruginosa in the airways of patients with chronic respiratory diseases, including cystic fibrosis. This results in long-term persistence, poorer clinical outcomes, and limited therapeutic options. In this study, we demonstrate that at physiological concentrations, sex steroids, including testosterone and estriol, induce membrane stress responses in P. aeruginosa. This is characterized by increased virulence and consequent inflammation and release of proinflammatory outer membrane vesicles promoting in vivo persistence of the bacteria. The steroid-induced P. aeruginosa response correlates with the molecular polarity of the hormones and membrane fluidic properties of the bacteria. This novel mechanism of interaction between sex steroids and P. aeruginosa explicates the reported increased disease severity observed in females with cystic fibrosis and provides evidence for the therapeutic potential of the modulation of sex steroids to achieve better clinical outcomes in patients with hormone-responsive strains. IMPORTANCE Molecular mechanisms by which sex steroids interact with P. aeruginosa to modulate its virulence have yet to be reported. Our work provides the first characterization of a steroid-induced membrane stress mechanism promoting P. aeruginosa virulence, which includes the release of proinflammatory outer membrane vesicles, resulting in inflammation, host tissue damage, and reduced bacterial clearance. We further demonstrate that at nanomolar (physiological) concentrations, male and female sex steroids promote virulence in clinical strains of P. aeruginosa based on their dynamic membrane fluidic properties. This work provides, for the first-time, mechanistic insight to better understand and predict the P. aeruginosa related response to sex steroids and explain the interindividual patient variability observed in respiratory diseases such as cystic fibrosis that are complicated by gender differences and chronic P. aeruginosa infection.

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“…It also appears to be driven by promoter elements that are the consensus for a ECF sigma factor (left panel, Figure 5) [28]. This data is also replicated in the Clinical isolate FRD1 and its derivative FRD440 [29]. Now we have a very substantial study to show enhanced expression of the dsbA gene, accumulation of the protein DsbA (Proteomic data) under mucoid conditions (AlgT+ scenario).…”

Section: A Close Look At the Dsba Gene/operon: Primer Extensionsmentioning

confidence: 56%

The Role ofPseudomonas aeruginosaDsbA-1 in Bacterial Pathogenesis: Current Research and Future Prospects

Kumar¹,

Malhotra²

2024

Pseudomonas Aeruginosa- New Perspectives and Applications

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Disulfide bond isomerase proteins (Dsbs) have been extensively characterized in gram-negative bacteria. Recently research efforts is being placed on their biology in gram-positive species. Modern “omics” technologies, allowed assessment of the contribution of the Dsbs to bacterial pathogenesis. The author cloned and characterized the dsbA 1 protein from Pseudomonas aeruginosa in the late 1990s. The global proteome analysis demonstrated that the dsbA gene is under the direct regulatory control of the extracytoplasmic function (ECF) sigma factor AlgT(U) or sigma-22. This is unique to P. aeruginosa. Disruption of dsbA gene results in pleiotropic phenotype: defect in assembly of cysteine disulfide bond containing proteins-as shown in many others. Recently, omics-based approaches identified expression changes in dsbA gene under different physiological states of bacterial pathogens-primarily in free-living, biofilm state, or under infectious disease conditions. Involvement of dsbA function in biofilm formation was shown using dsbA gene disruption mutants. This chapter documents past and current findings and concludes with future trends in research on Dsbs including peptidomimetics.

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Nonmucoid conversion of mucoidPseudomonas aeruginosainduced by sulfate-stimulated growth

Cited by 10 publications

References 46 publications

Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm

Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm

Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa

The Role ofPseudomonas aeruginosaDsbA-1 in Bacterial Pathogenesis: Current Research and Future Prospects

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