Nonmutagenic carcinogens induce intrachromosomal recombination in dividing yeast cells.

Schiestl, Robert H.

doi:10.1289/ehp.93101s5179

Cited by 10 publications

(1 citation statement)

References 31 publications

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“…Cyclophosphamide is an alkylating agent which can result in guanine adducts in the DNA, mitomycin C is an alkalating cross-linking agent, etoposide is a topoisomerase II inhibitor, and X-radiation causes results in DNA strand breaks and free radicals which can damage DNA. Methylene chloride was chosen for study because it is known to be carcinogenic in animals and causes intrachromosomal recombination but not interchromosomal recombination in yeast (Scheistl, 1993). The induction of inversions in response to all of these agents suggested that chromosomal inversion was a common mutational response to different DNA damaging agents with different mechanisms of genotoxic action.…”

Section: The Pkz1 Assay As An In Vivo Mutation Assaymentioning

confidence: 99%

The PKZ1 Recombination Mutation Assay: A Sensitive Assay for Low Dose Studies

2006

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The majority of mutation studies are performed at high doses of DNA damaging agents due to the insensitivity of most mutation assays. Extrapolation using a linear no-threshold (LNT) dose response model is then used to estimate the extent of possible DNA damage at lower doses. There is increasing evidence to suggest that the LNT model may not be correct at low doses of at least some DNA damaging agents. The pKZ1 in vivo and in vitro recombination assays have proven to be very sensitive for detection of changes in chromosomal inversion in lymphoid tissue in response to low doses of DNA damaging agents. Non-linear dose response curves for chromosomal inversion as an end-point have been identified at low doses of DNA damaging agents using this assay. Here, we review the inversion results obtained to date with the pKZ1 assays and discuss their suitability for low dose studies.

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Section: The Pkz1 Assay As An In Vivo Mutation Assaymentioning

confidence: 99%