Nonnegative principal component analysis in thin layer fingerprint screening: A case of <i>Gentiana</i> extracts from <i>in vitro</i> cultures

Gadowski, Sebastian; Tomiczak, Karolina; Komsta, Łukasz

doi:10.1080/10826076.2022.2089998

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…However, this method results in very weak chromatograms with unacceptable sensitivity. erefore, we decided to perform liquid extraction, evaporate the extract, and dissolve it in methanol, similar to the method followed in our previous TLC study (Gadowski et al, 2022). Although there was a risk of loss of volatile compounds, the resulting chromatogram had many more peaks.…”

Section: Discussionmentioning

confidence: 99%

“…ese facts encouraged us to analyze a representative set of in vitro cultured gentian species. Our previous research (Gadowski et al, 2022) focused on thin layer chromatography (TLC). is study is a continuation of this research using headspace GC, which is a broad and common technique used to analyze various types of samples (Wang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Discriminant Analysis of Gentiana Headspace Gas Chromatography – Mass Spectrometry Extract Data from In Vitro Cultures

Gadowski,

Tomiczak,

Pietraś

et al. 2023

Acta Agrobot

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A set of Gentiana L. species was successfully grown in vitro under the same conditions, and 72 samples from various cultures of these species (root, shoot, cotyledon callus, hypocotyl callus, and root callus) were obtained. The investigated species were G. affinis, G. andrewsii, G. bhutanica, G. burseri, G. cachemirica, G. capitata, G. crassicaulis, G. dahurica, G. decumbens, G. freyniana, G. frigida, G. gelida, G. grossheimii, G. kurroo, G. macrophylla, G. paradoxa, G. robusta, G. scabra, G. septemfida, G. siphonantha, and G. tianschanica. The obtained samples were extracted with a methanol-acetone-water (3:1:1) mixture, evaporated to dryness, and subjected to thin layer chromatography (TLC) on silica gel in sandwich mode with ethyl acetate-methanol-water (8:2:2) as the mobile phase. The resulting dry extracts were subjected to gas chromatography – mass spectrometry (GC-MS) fingerprinting of the headspace volatile fraction. Total ion count and average mass spectrum vectors were collected as two blocks and scaled independently to form a complex dataset. The major direction separating root or shoot samples from callus samples was found not to be fully associated with the highest variance as this information was placed in the first and fourth principal components of the principal component analysis (PCA). Therefore, linear discriminant analysis was performed on the first four (only the informative) components to reveal features responsible for the separation of culture types in the multivariate space.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discriminant Analysis of Gentiana Headspace Gas Chromatography – Mass Spectrometry Extract Data from In Vitro Cultures

Gadowski,

Tomiczak,

Pietraś

et al. 2023

Acta Agrobot

Self Cite

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High dynamic range in videodensitometry—a comparative study to classic videoscanning on Gentiana extracts

Gadowski

Tomiczak

Komsta

2023

JPC-J Planar Chromat

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The advantages of high dynamic range (HDR) imaging in videodensitometry are presented and discussed on the example of Gentiana extract thin-layer fingerprints visualized under 254 nm. An inexpensive microscope camera, together with security surveillance lens, mounted instead of original camera on videodensitometry chamber, allows to grab HDR images with high tonal range using Python scripts and OpenCL library. HDR imaging preserves linearity in whole tonal range and does not destroy details in the brightest and darkest plate regions, so it can be seen as a good alternative to classical videodensitometry. Moreover, the tonemapping of HDR images can be used to present a plate photograph with enhanced visibility of weak spots and other details. Principal component analysis done on nine classic exposures and HDR image proves that HDR image contains the highest amount of extracted information from the thin-layer chromatographic plate.

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Nonnegative principal component analysis in thin layer fingerprint screening: A case of Gentiana extracts from in vitro cultures

Cited by 2 publications

References 15 publications

Discriminant Analysis of Gentiana Headspace Gas Chromatography – Mass Spectrometry Extract Data from In Vitro Cultures

Discriminant Analysis of Gentiana Headspace Gas Chromatography – Mass Spectrometry Extract Data from In Vitro Cultures

High dynamic range in videodensitometry—a comparative study to classic videoscanning on Gentiana extracts

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