2018
DOI: 10.1101/264283
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Nonpolar Residues in the Presumptive Pore-Lining Helix of Mechanosensitive Channel MSL10 Influence Channel Behavior and Confirm a Non-Conducting Function

Abstract: 25Mechanosensitive (MS) ion channels provide a universal mechanism for sensing and responding 26 to increased membrane tension. MscS-Like(MSL)10 is a relatively well-studied MS ion channel 27 from Arabidopsis thaliana that is implicated in cell death signaling. The relationship between the 28 amino acid sequence of MSL10 and its conductance, gating tension, and opening and closing 29 kinetics remain unstudied. Here we identify several nonpolar residues in the presumptive pore-30 lining transmembrane helix o… Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

0
2
0

Year Published

2018
2018
2018
2018

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(2 citation statements)
references
References 68 publications
0
2
0
Order By: Relevance
“…Point mutations in the pore-lining helix of EcMscS caused a faster inactivation, which is also observed at low pH (6.0) [37]. Likewise, residues of the homologous TM6 of AtMSL10 seem important to stabilize the open state as point mutations lead to flickery channel activity [38]. Therefore, the inactivation of EcMscS is governed by interactions with residues in the TM3 helix.…”
Section: Inactivation Kineticsmentioning
confidence: 99%
See 1 more Smart Citation
“…Point mutations in the pore-lining helix of EcMscS caused a faster inactivation, which is also observed at low pH (6.0) [37]. Likewise, residues of the homologous TM6 of AtMSL10 seem important to stabilize the open state as point mutations lead to flickery channel activity [38]. Therefore, the inactivation of EcMscS is governed by interactions with residues in the TM3 helix.…”
Section: Inactivation Kineticsmentioning
confidence: 99%
“…Therefore, the inactivation of EcMscS is governed by interactions with residues in the TM3 helix. Likewise, residues of the homologous TM6 of AtMSL10 seem important to stabilize the open state as point mutations lead to flickery channel activity [38].…”
Section: Inactivation Kineticsmentioning
confidence: 99%