Nonprotein sulfhydryl compounds in canine gastric mucosa: effects of PGE2 and ethanol

Miller, Thomas A.; Li, Donghui; Kuo, Y. M.; Schmidt, Klaus; Shanbour, Linda L.

doi:10.1152/ajpgi.1985.249.1.g137

Cited by 35 publications

(24 citation statements)

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“…In NEM pretreated animals treated with AML, there were a significant increased in the mean score of blood congestion and necrosis when compared with saline pretreated group treated with AML. This is in agreement with a few studies which have been reported previously that ethanol-induced gastric lesions will lead to decrease of sulfhydryl compounds in the gastric mucosa (Szabo et al, 1981;Miller et al, 1985 ;Tariq & Aqeel, 1990 Therefore, from the current histology result in ethanol-induced gastric mucosal lesion treated with AML in NEM-pretreated rats, there was significant increment of certain inflammatory features, indicating the worsening of the ulcer when compared with the group treated with AML in saline pretreated group. Indeed, this indicates a strong involvement of SHs pathway in gastroprotection of AML (Figure 4a) which also in agreement with the result of macroscopic done previously.…”

Section: Discussionsupporting

confidence: 92%

Antinociceptive and anti-ulcerogenic activities of the ethanolic extract of Annona muricata leaf

Hamid¹,

Foong²,

Ahmad³

et al. 2012

Rev. bras. farmacogn.

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Ethanolic extract of Annona muricata L., Annonaceae, leaf (AML) was used to investigate its antinociceptive and anti-ulcerogenic activities and the involvement of the mechanism of ethanolic leaves extract of AML in various animal models. Antinociceptive activity of AML extract was done using acetic acid-induced abdominal writhing in mice, formalin test in rats and hot plate test in mice. Furthermore, the antiulcerogenic effect of AML extract was studied in ethanol-induced ulcer model in rats, ethanol-induced gastric lesions in L-NAME-pre-treated rats as well as ethanol-induced gastric lesions in NEM-pre-treated rats test model to determine its mechanism. AML exhibited signifi cant and dose-dependent antinociceptive activity. It also signifi cantly decreased the ulcerative lesion produced by ethanol in rats in a dose-dependent manner. Pre-treatment with N-ethymaleimide, a thiol blocker, including mucosal nonprotein sulfhydryl groups, reduced the anti-ulcerogenic effect of AML extract in the same ulcer model, suggesting that AML extract may have active substances such as tannins, fl avanoids and triterpenes that increase the mucosal nonprotein sulfhydryl group content.

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Section: Discussionsupporting

confidence: 92%

Antinociceptive and anti-ulcerogenic activities of the ethanolic extract of Annona muricata leaf

Hamid¹,

Foong²,

Ahmad³

et al. 2012

Rev. bras. farmacogn.

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“…This increase in the gastric mucosal LPO concentration with the development of gastric mucosal lesions induced by WIR stress was similar to that shown by Yoshikawa et al [4] and Itoh et al [9]. It has been dem onstrated that SH compounds in the stomach might be important for the maintenance of gastric mucosal integri ty [35][36][37][38] and that about 95% of NP-SH in the stomach is GSH which functions as a scavenger of H^O; and/or lipid hydroperoxides via GSH-px in cells [39], In rats with WIR stress over a 6-hour period, the gastric mucosal NP-SH concentration decreased to about half the level of con trol rats without WIR stress at an early stage of the lesion, i.e" 3 h after the onset of WIR, and a further decrease in that concentration scarcely occurred at a progressed stage of the lesion, i.e., 6 h after the onset of WIR (table 1; fig. 1).…”

Section: Discussionsupporting

confidence: 86%

Involvement of the Xanthine-Xanthine Oxidase System and Neutrophils in the Development of Acute Gastric Mucosal Lesions in Rats with Water Immersion Restraint Stress

et al. 1997

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In rats subjected to water immersion restraint (WIR) stress for 1, 3, and 6 h, gastric mucosal lesions developed time-dependently with an increase in lipid peroxide (LPO) levels and a decrease in nonprotein sulfhydryl levels in the gastric mucosa. The gastric mucosal xanthine oxidase (XO) activity significantly increased with the conversion of xanthine dehydrogenase (XD) to XO at 6 h of WIR (3.2-fold that of the control group without WIR). A significant increase in myeloperoxidase (MPO) activity, an index of neutrophil infiltration, occurred in the gastric mucosa at 3 and 6 h of the WIR (2.2- and 3.3-fold that of the control group without WIR, respectively). In contrast, superoxide dismutase, catalase, and glutathione peroxidase activities in the gastric mucosa did not change during the WIR period. Pretreatment with either allopurinol (AP), an inhibitor of XO, or soybean trypsin inhibitor (STI), a serine protease inhibitor, attenuated the lesion development at 6 h of WIR, but not at 3 h. In the gastric mucosa of rats pretreated with AP, enhancements of LPO formation, sulfhydryl oxidation, and XO activity found at 6 h of WIR were prevented with inhibition of XD plus XO activity, while in the gastric mucosa of rats pretreated with STI, these enhancements were prevented with inhibition of the conversion of XD to XO. In the gastric mucosa of rats pretreated with anti-polymorphonuclear leukocyte antiserum, the lesion development and enhanced LPO formation and sulfhydryl oxidation found at 3 and 6 h of WIR were prevented with a decrease in increased MPO activity. These results indicate that in the gastric mucosa of rats with WIR stress, the progression of lesions is mainly related to enhanced LPO formation and sulfhydryl oxidation which depend on an increased generation of oxygen free radicals via the xanthine-XO system and neutrophils rather than the change in the oxygen free radical-scavenging activity of antioxidant enzymes. The present results also suggest that increased gastric mucosal LPO formation and sulfhydryl oxidation found at 3 h of WIR could be mainly due to neutrophil-derived oxygen free radicals, while enhanced gastric mucosal LPO formation and sulfhydryl oxidation found at 6 h of WIR could be due to both neutrophil- and XO-derived oxygen free radicals.

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“…Szabo et al (16), Ezer (17) and Miller et al (18) proposed that sulfhydryl compounds in the gastric mucosa play an important role on the cytoprotection seen with several agents. However, there are controversial data about the role of sulfhydryl compounds (mainly reduced glutathione) in the role of gastric cytoprotection.…”

Section: Discussionmentioning

confidence: 99%

Cytoprotective Effects of NC-1300 and Omeprazole on HCl⋅Ethanol-Induced Gastric Lesions in Rats

Okabe¹,

Awane²

1986

Japanese Journal of Pharmacology

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Nonprotein sulfhydryl compounds in canine gastric mucosa: effects of PGE2 and ethanol

Cited by 35 publications

References 0 publications

Antinociceptive and anti-ulcerogenic activities of the ethanolic extract of Annona muricata leaf

Antinociceptive and anti-ulcerogenic activities of the ethanolic extract of Annona muricata leaf

Involvement of the Xanthine-Xanthine Oxidase System and Neutrophils in the Development of Acute Gastric Mucosal Lesions in Rats with Water Immersion Restraint Stress

Cytoprotective Effects of NC-1300 and Omeprazole on HCl⋅Ethanol-Induced Gastric Lesions in Rats

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