2013
DOI: 10.1371/journal.pone.0061240
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Nonsense-Mediated mRNA Decay Controls the Changes in Yeast Ribosomal Protein Pre-mRNAs Levels upon Osmotic Stress

Abstract: The expression of ribosomal protein (RP) genes requires a substantial part of cellular transcription, processing and translation resources. Thus, the RP expression must be tightly regulated in response to conditions that compromise cell survival. In Saccharomyces cerevisiae cells, regulation of the RP gene expression at the transcriptional, mature mRNA stability and translational levels during the response to osmotic stress has been reported. Reprogramming global protein synthesis upon osmotic shock includes t… Show more

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Cited by 26 publications
(22 citation statements)
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“…To obtain a more quantitative assessment of transcript levels, rather than just assessing the ratio between the different spliced forms, we analyzed the same samples by northern blot. This analysis showed that cells treated in heat shock conditions resulted in much weaker signal than in the samples obtained from cells grown at 25°C, consistent with a general down-regulation of ribosomal protein genes under stress [43], [44], [45], [46], [47]. Upon NMD inactivation, we observed a rescue of transcript levels, which mostly corresponded to unspliced RNAs and to some alternatively spliced transcripts (Fig.…”
Section: Resultssupporting
confidence: 78%
“…To obtain a more quantitative assessment of transcript levels, rather than just assessing the ratio between the different spliced forms, we analyzed the same samples by northern blot. This analysis showed that cells treated in heat shock conditions resulted in much weaker signal than in the samples obtained from cells grown at 25°C, consistent with a general down-regulation of ribosomal protein genes under stress [43], [44], [45], [46], [47]. Upon NMD inactivation, we observed a rescue of transcript levels, which mostly corresponded to unspliced RNAs and to some alternatively spliced transcripts (Fig.…”
Section: Resultssupporting
confidence: 78%
“…In this regard, it is worth noting that—in Saccharomyces cerevisiae —evidence suggests that osmotic stress increases NMD magnitude [163, 164]. This may be a mechanism to degrade ribosomal protein mRNAs by NMD and thereby redirect the translational apparatus to stress-response mRNAs, many of which are immune to NMD.…”
Section: Perspectivesmentioning
confidence: 99%
“…Total RNA extraction and cellular mRNA levels were determined as previously described with a few modifications (60). Briefly, 10 to 20 ml of exponentially growing cells was collected by centrifugation, washed with ice-cold water, and frozen at Ϫ80°C.…”
Section: Methodsmentioning
confidence: 99%