Nonsolvent-induced phase separation of poly(3-hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) blend as a facile platform to fabricate versatile nanofiber gels: Aero-, hydro-, and oleogels

Kang, Jiseon; Choi, Jiwon; Yun, Seok Il

doi:10.1016/j.ijbiomac.2021.01.106

Cited by 10 publications

(9 citation statements)

References 53 publications

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“…After 24 hours of incubation, 0.5 mg /ml MTT solution was added to the plates and incubated for up to 4 h. Then DMSO solvent was added to them and placed on a shaker for 20 minutes (with light protection). The intensity of the purple dye produced at the wavelength of 570 nm was read and the number of living cells was determined relatively by absorbing the samples 32,33 .…”

Section: Cytotoxicity Test (Mtt)mentioning

confidence: 99%

Ultrathin Carboxymethyl Cellulose/ Fish Scale Collagen Based Hydrogel Films: Vitamin E Loading, Controlled Release, Antibacterial, and Antioxidant Activities

Zallaghi,

Esmkhani,

Javanshir

2024

Preprint

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Section: Cytotoxicity Test (Mtt)mentioning

confidence: 99%

Ultrathin Carboxymethyl Cellulose/ Fish Scale Collagen Based Hydrogel Films: Vitamin E Loading, Controlled Release, Antibacterial, and Antioxidant Activities

Zallaghi,

Esmkhani,

Javanshir

2024

Preprint

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“…3−11 These polymers readily undergo thermoreversible gelation in various solvents by forming gel networks made up of polymer crystalline phases, which are thermally labile. [3][4][5][6][7][8][9][10]12,13 The inclusion of crystalline networks within the gels and aerogels provides the required mechanical robustness, flexibility, and adequate porosity. The solvent extraction by suitable procedures from thermoreversible gels leads to the corresponding aerogels.…”

Section: ■ Introductionmentioning

confidence: 99%

“…[3][4][5][6][7][8][9][10][11]13 Among these, biodegradable polymer-based thermoreversible gels and aerogels gained attention due to their unique applications in the biomedical field to biodegradable packaging. 5,9,10,14,15 Polymer aerogels are generally produced by freeze-drying or supercritical drying methods and they exhibit less entangled amorphous chains compared to their bulk counterparts. 2,7,13,15 Recently, our group investigated the phase transition behavior of semicrystalline polymers in gels and aerogels and observed unusual phase transitions in syndiotactic polystyrene (sPS), nylon-11 and poly(vinylidene fluoride).…”

Section: ■ Introductionmentioning

confidence: 99%

“…The addition of nonsolvent into the PHB solution also resulted in the gelation by the nonsolvent-induced phase separation (NIPS) method. 9,10 The solvent/nonsolvent combinations like chloroform/THF, chloroform/1, 4-dioxane, etc., can form PHB gels by the NIPS method. The careful extraction of the solvent by supercritical drying or freeze-drying results in the corresponding aerogels.…”

Section: ■ Introductionmentioning

confidence: 99%

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Unprecedented Thermally Induced Structural Changes in Aerogels of Poly(3-hydroxybutyrate) during Heating

Akhila,

G. Krishnan,

Parukoor Thomas

et al. 2024

ACS Appl. Polym. Mater.

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Thermally induced crystal structure changes in semicrystalline polymers involve solid-to-solid transition or melting of the starting crystal form followed by the recrystallization into different crystal forms. Understanding such crystal-to-crystal transitions by controlling the chain mobility of amorphous chains is rarely studied. Herein, we chose poly(3-hydroxybutyrate) (PHB) to investigate the temperature-induced structural changes in the bulk and aerogel samples and present the role of amorphous chain mobility on structural reorganization during heating. Aerogels of PHB were prepared by freeze-drying the thermoreversible gels and the films were prepared by hot pressing the PHB pellets. Both aerogels and films crystallized into the α form. We observed a major structural reorganization in aerogels upon heating prior to the melting and such a transition was not observed in the meltcrystallized PHB α form. We speculate that the enhanced mobility of the tie chains that are present between the α lamellar crystals during the heating of the aerogel triggered the conformational change from T 2 G 2 helical chains to all-trans conformation (T′TT̅ ′T̅ ) within the crystal lattice resulted in the crystal-to-crystal (α to α + β) transition.

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“…It is worth noting that in studies investigating the effects of porosity in substrates produced via NIPS, the majority of work has focused predominantly on membranes, with the thicknesses ranging from 70 to 250 μm. ,− Very few studies have investigated thicker (i.e., of thickness in the range of mm or more), nonmembranous substrates. , Furthermore, most studies have tended to characterize or provide a limited number of cross-sectional views of their substrates using SEM, overlooking the rest of the internal structure. In light of these, this study represents an initial effort to fabricate substrates tailored for bone tissue engineering rather than for thin membranes.…”

Section: Introductionmentioning

confidence: 99%

Microstructural Characterization of PCL-HA Bone Scaffolds Based on Nonsolvent-Induced Phase Separation

Aydin,

Sahin,

Dogan

et al. 2023

ACS Omega

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Composite materials containing pores play a crucial role in the field of bone tissue engineering. The nonsolvent-induced phase separation (NIPS) technique, commonly used for manufacturing membranes, has proven to be an effective method for fabricating composite scaffolds with tunable porosity. To explore this potential, we produced 10% (w/v) poly(caprolactone) (PCL)-nanohydroxyapatite (HA) composite porous film scaffolds with varying HA contents (0/10/15/20 wt %) and two thicknesses (corresponding to 1 and 2 mL of solution resulting in 800–900 and 1600–1800 μm thickness, respectively) using the NIPS method. We conducted a comprehensive analysis of how the internal microstructure and surface characteristics of these scaffolds varied based on their composition and thickness. In particular, for each scaffold, we analyzed overall porosity, pore size distribution, pore shape, and degree of anisotropy as well as mechanical behaviors. Micro-CT and SEM analyses revealed that PCL-HA scaffolds with various HA contents possessed micro (<100 μm) scale porosity due to the NIPS method. Greater thicknesses typically resulted in larger average pore sizes and greater overall porosity. However, unlike in thinner scaffolds, greater/higher HA content did not exhibit a direct correlation with a greater pore size for thicker scaffolds. In thinner scaffolds, adding HA above an effective threshold content of 15 wt % and beyond did lead to a greater pore size. The higher pore anisotropy was in line with the higher HA content for both groups. SEM images demonstrated that both groups showed highly uniformly distributed internal microporous morphology regardless of HA content and thickness. The results suggest that NIPS-based scaffolds hold promise for bone tissue engineering but that the optimal HA content and thickness should be carefully considered based on desired porosity and application.

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Nonsolvent-induced phase separation of poly(3-hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) blend as a facile platform to fabricate versatile nanofiber gels: Aero-, hydro-, and oleogels

Cited by 10 publications

References 53 publications

Ultrathin Carboxymethyl Cellulose/ Fish Scale Collagen Based Hydrogel Films: Vitamin E Loading, Controlled Release, Antibacterial, and Antioxidant Activities

Ultrathin Carboxymethyl Cellulose/ Fish Scale Collagen Based Hydrogel Films: Vitamin E Loading, Controlled Release, Antibacterial, and Antioxidant Activities

Unprecedented Thermally Induced Structural Changes in Aerogels of Poly(3-hydroxybutyrate) during Heating

Microstructural Characterization of PCL-HA Bone Scaffolds Based on Nonsolvent-Induced Phase Separation

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