Nonsporulating Bacterial Pathogens

Bucher, G. E.

doi:10.1016/b978-0-12-395603-3.50008-2

Cited by 39 publications

(21 citation statements)

References 44 publications

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“…For example, one cultured isolate from the gypsy moth midgut (NAB5) is highly similar (Ͼ99%) to a sequence found in the gut of the sheep mite, Psoroptes ovis (48), and both match Serratia marcesens to the species level (Ͼ98%). Similar sequences have been found in most insects and other arthropods (12,35,67). Serratia species are generally considered pathogens and can cause disease in insect production facilities (35,68).…”

Section: Discussionsupporting

confidence: 58%

Census of the Bacterial Community of the Gypsy Moth Larval Midgut by Using Culturing and Culture-Independent Methods

Broderick

Raffa²,

Goodman

et al. 2004

Appl Environ Microbiol

466

419

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Little is known about bacteria associated with Lepidoptera, the large group of mostly phytophagous insects comprising the moths and butterflies. We inventoried the larval midgut bacteria of a polyphagous foliivore, the gypsy moth (Lymantria dispar L.), whose gut is highly alkaline, by using traditional culturing and cultureindependent methods. We also examined the effects of diet on microbial composition. Analysis of individual third-instar larvae revealed a high degree of similarity of microbial composition among insects fed on the same diet. DNA sequence analysis indicated that most of the PCR-amplified 16S rRNA genes belong to the ␥-Proteobacteria and low G؉C gram-positive divisions and that the cultured members represented more than half of the phylotypes identified. Less frequently detected taxa included members of the ␣-Proteobacterium, Actinobacterium, and Cytophaga/Flexibacter/Bacteroides divisions. The 16S rRNA gene sequences from 7 of the 15 cultured organisms and 8 of the 9 sequences identified by PCR amplification diverged from previously reported bacterial sequences. The microbial composition of midguts differed substantially among larvae feeding on a sterilized artificial diet, aspen, larch, white oak, or willow. 16S rRNA analysis of cultured isolates indicated that an Enterococcus species and culture-independent analysis indicated that an Entbacter sp. were both present in all larvae, regardless of the feeding substrate; the sequences of these two phylotypes varied less than 1% among individual insects. These results provide the first comprehensive description of the microbial diversity of a lepidopteran midgut and demonstrate that the plant species in the diet influences the composition of the gut bacterial community.

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Section: Discussionsupporting

confidence: 58%

Census of the Bacterial Community of the Gypsy Moth Larval Midgut by Using Culturing and Culture-Independent Methods

Broderick

Raffa²,

Goodman

et al. 2004

Appl Environ Microbiol

466

419

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“…Enterobacter has often been associated with insect diseases and this genus contains insect pathogenic strains (Grimont and Grimont, 1978) which are usually considered opportunistic or facultative pathogens as these are often avirulent to insects when present in digestive tract but are lethal upon entering insect haemocoel following injury or stress (Bucher, 1963). There are two mechanism for insect killing by bacteria, first via toxin alone, which does not induce sepsis but causes starvation and second via sepsis caused by microbiota (more specifically by Enterobacter and Enterococcous spp.)…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of bacteria isolated from gut of Spodoptera litura (Lepidoptera: Noctuidae) and fitness costs of insect associated with consumption of bacteria

Thakur

Dhammi

Saini

et al. 2015

Journal of Invertebrate Pathology

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“…For entomogenous bacteria, Steinhaus (1949) provides a good general introduction to the topic, and more specific coverage is provided by Bucher (1963), Dutky (1963), Heimpel and Angus (1963), and Lysenko (1963). The use of bacteria for insect control was discussed by Falcon (1971), while other subjects on the toxins and host spectrum of B. thuringiensis were included in Burges and Hussey's Microbial Control of Insects and Mites (1971) and updated in Burges (1981).…”

Section: Literaturementioning

confidence: 99%

Laboratory Guide to Insect Pathogens and Parasites

Poinar¹,

Thomas²

1984

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Dedicated to the memory of ROBERT VAN DEN BOSCH, for his interest, encouragement, and tenacity in promoting biological pest control A. Dark red color of a larva of the wax moth, Gal/eria mel/onel/a, infected with Heterorhabditis nematodes. The color is produced by the bacterium Xenorhabdus /uminescens, which is symbiotically associated with nematodes of the genus Heterorhabditis. Another insect pathogen that produces a red color in the host is the bacterium Serratia marcescens.

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Nonsporulating Bacterial Pathogens

Cited by 39 publications

References 44 publications

Census of the Bacterial Community of the Gypsy Moth Larval Midgut by Using Culturing and Culture-Independent Methods

Census of the Bacterial Community of the Gypsy Moth Larval Midgut by Using Culturing and Culture-Independent Methods

Pathogenicity of bacteria isolated from gut of Spodoptera litura (Lepidoptera: Noctuidae) and fitness costs of insect associated with consumption of bacteria

Laboratory Guide to Insect Pathogens and Parasites

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