Nonsurgical embryo transfer in the common marmoset monkey

Marshall, Vivienne S.; Kalishman, Jennifer; Thomson, James A.

doi:10.1111/j.1600-0684.1997.tb00218.x

Cited by 21 publications

(19 citation statements)

References 27 publications

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“…The mRNA-injected embryos were cultured in ISM-1 medium (Origio) for 4 days at 37 C with 5% CO 2 , 5% O 2 , and 90% N 2 . The live embryos were then noninvasively transferred into the surrogate mothers' uteri as previously described (Marshall et al, 1997;Takahashi et al, 2014). The plasma progesterone levels of the recipients were measured once per week to monitor pregnancy.…”

Section: Microinjection and Embryo Transfermentioning

confidence: 99%

Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing

et al. 2016

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Recent advances in genome editing have facilitated the generation of nonhuman primate (NHP) models, with potential to unmask the complex biology of human disease not revealed by rodent models. However, their broader use is hindered by the challenges associated with generation of adult NHP models as well as the cost of their production. Here, we describe the generation of a marmoset model of severe combined immunodeficiency (SCID). This study optimized zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) to target interleukin-2 receptor subunit gamma (IL2RG) in pronuclear stage marmoset embryos. Nine of 21 neonates exhibited mutations in the IL2RG gene, concomitant with immunodeficiency, and three neonates have currently survived from 240 days to 1.8 years. Our approach demonstrates highly efficient production of founder NHP with SCID phenotypes, with promises of multiple pre-clinical and translational applications.

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Section: Microinjection and Embryo Transfermentioning

confidence: 99%

Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing

et al. 2016

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“…For example, a third of the world deer species is rare or endangered but the reproductive physiology is supposed to be close to the common species studied for farming [3]. There are other models such as domestic cattle for wild oxen [62], domestic cats for endangered felids [54], domestic dogs for foxes [17], common marmoset monkeys for endangered callithrix species [43], and South American camelids for endangered camelids [6]. The domestic ferret (Mustela putorius furo) and domestic rabbit (Oryctolagus cuniculus) have also been studied for developing non-surgical methods of when using reproductive biotechnologies for endangered domestic breeds.…”

Section: Few Individuals Are Available and Sometimes In Widely Disparmentioning

confidence: 99%

Reproductive biotechnologies for endangered mammalian species

2000

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-Assisted reproductive techniques (gamete cryopreservation, artificial insemination, embryo transfer, and in vitro fertilization) allow to propagate small fragmented populations of wild endangered species or domestic breeds. There are the best way for producing several offspring from selected genitors in order to avoid inbreeding depression. However, few mammalian species have been well studied for their reproductive biology whereas huge differences have been observed between these species. Furthermore, materials, methods and experimental designs have to be adapted for each case and each limiting factor (wildness, poor quantity of biological material, disparate locations). Genome resource banking is currently arising and the most applied reproductive biotechnology remains artificial insemination. Assisted reproductive techniques currently developed in domestic species (intracytoplasmic sperm injection, nuclear transfer) may offer new opportunities for the propagation of endangered species. endangered species / artificial insemination / cryopreservation / in vitro fertilization / embryo Résumé -Biotechnologies de la reproduction appliquées aux mammifères en voie de disparition. Les techniques de reproduction assistée (cryoconservation des gamètes, insémination artificielle, transfert embryonnaire, fécondation in vitro) permettent d'accroître des populations, parfois dispersées géographiquement, d'espèces sauvages ou domestiques en voie de disparition. Ces méthodes sont le meilleur moyen pour produire plusieurs descendants à partir de géniteurs sélectionnés de façon à éviter la consanguinité. La biologie de la reproduction est connue pour peu d'espèces de mammifères alors que de grandes différences ont pourtant été mises en évidence entre ces espèces. En outre, les matériels, les méthodes, ainsi que les schémas expérimentaux doivent être adaptés à chaque cas et pour chaque facteur limitant (animaux sauvages, peu de matériel biologique, populations dispersées). Des banques génétiques sont actuellement en voie de constitution mais la technique la plus utilisée reste l'insémination artificielle. Cependant, des biotechnologies de la reproduction en cours de mise au point chez les animaux domestiques (injection intra-cytoplasmique de spermatozoïde, transfert nucléaire) pourraient offrir de nouvelles possibilités pour l'accroissement de populations menacées. espèces menacées / insémination artificielle / congélation / fécondation in vitro / embryon Reprod. Nutr. Dev. 40 (2000)

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“…Unlike macaques, marmosets routinely deliver twins or triplets for each pregnancy. In addition, it is possible to synchronize the marmoset ovarian cycle with prostaglandin analogs, collect age-matched embryos from multiple females, and transfer embryos to synchronized recipients with success rates in the range of 70%-80% [1][2][3]. Because these reproductive characteristics allow routine efficient transfer of multiple embryos, marmosets constitute an excellent primate species for the generation of transgenic and knockout animal models of human diseases.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Novel Embryonic Stem Cell Lines Derived from the Common Marmoset ( Callithrix jacchus )

et al. 2005

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The successful establishment of human embryonic stem cell (hESC) lines has inaugurated a new era in regenerative medicine by facilitating the transplantation of differentiated ESCs to specific organs. However, problems with the safety and efficacy of hESC therapy in vivo remain to be resolved. Preclinical studies using animal model systems, including nonhuman primates, are essential to evaluate the safety and efficacy of hESC therapies. Previously, we demonstrated that common marmosets are suitable laboratory animal models for preclinical studies of hematopoietic stem cell therapies. As this animal model is also applicable to preclinical trials of ESC therapies, we have established novel common marmoset ESC (CMESC) lines. To obtain marmoset embryos, we developed a new embryo collection system, in which blastocysts can be obtained every 3 weeks from each marmoset pair. The inner cell mass was isolated by immunosurgery and plated on a mouse embryonic feeder layer. Some of the CMESC lines were cultured continuously for more than 1 year. These CMESC lines showed alkaline phosphatase activity and expressed stage-specific embryonic antigen (SSEA)-3, SSEA-4, TRA-1-60, and TRA-1-81. On the other hand, SSEA-1 was not detected. Furthermore, our novel CMESCs are pluripotent, as evidenced by in vivo teratoma formation in immunodeficient mice and in vitro differentiation experiments. Our established CMESC lines and the common marmoset provide an excellent experimental model system for understanding differentiation mechanisms, as well as the development of regenerative therapies using hESCs. Stem Cells 2005;23:1304-1313 This material is protected by U.S.

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Nonsurgical embryo transfer in the common marmoset monkey

Cited by 21 publications

References 27 publications

Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing

Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing

Reproductive biotechnologies for endangered mammalian species

Establishment of Novel Embryonic Stem Cell Lines Derived from the Common Marmoset ( Callithrix jacchus )

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