Nonthermal plasma in dentistry: An update

Ranjan, Rajeev; Krishnamraju, Penumetsa Venkata; Shankar, Thatapudi; Gowd, Snigdha

doi:10.4103/jispcd.jispcd_29_17

Cited by 29 publications

(3 citation statements)

References 37 publications

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“…Presently, the most commonly researched applications within the field of plasma medicine are dentistry [13], dermatology [14,15], oncology [16], infection control, and sterilization [17][18][19]. However, there is further potential for applications of cold plasma treatments in a variety of other areas, including ophthalmological and neurological applications [20][21][22]. Some applications of plasma medicine extend beyond the realm of tissue or clinical treatments.…”

Section: History and Current State Of Plasma Medicinementioning

confidence: 99%

Cold Atmospheric Plasma Medicine: Applications, Challenges, and Opportunities for Predictive Control

Kazemi,

Nicol,

Bilén

et al. 2024

Plasma

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Plasma medicine is an emerging field that applies the science and engineering of physical plasma to biomedical applications. Low-temperature plasma, also known as cold plasma, is generated via the ionization of atoms in a gas, generally via exposure to strong electric fields, and consists of ions, free radicals, and molecules at varying energy states. Plasmas generated at low temperatures (approximately room temperature) have been used for applications in dermatology, oncology, and anti-microbial strategies. Despite current and ongoing clinical use, the exact mechanisms of action and the full range of effects of cold plasma treatment on cells are only just beginning to be understood. Direct and indirect effects of plasma on immune cells have the potential to be utilized for various applications such as immunomodulation, anti-infective therapies, and regulating inflammation. In this review, we combine diverse expertise in the fields of plasma chemistry, device design, and immunobiology to cover the history and current state of plasma medicine, basic plasma chemistry and their implications, the effects of cold atmospheric plasma on host cells with their potential immunological consequences, future directions, and the outlook and recommendations for plasma medicine.

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Section: History and Current State Of Plasma Medicinementioning

confidence: 99%

Cold Atmospheric Plasma Medicine: Applications, Challenges, and Opportunities for Predictive Control

Kazemi,

Nicol,

Bilén

et al. 2024

Plasma

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“…CAP's antibacterial potential largely stems from its ability to produce reactive species, including reactive oxygen and nitrogen species (RONS), that can impair and neutralize microorganisms [5][6][7]. Previous studies have reported the effects of a nitrogen-based CAP jet on the morphology of Streptococcus mutans using transmission electron microscopy [6,8]. Generally, a low pressure or a high temperature is required for plasma generation [7].…”

Section: Introductionmentioning

confidence: 99%

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Kim

2024

Biomedicines

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As biofilms mature, biomass and extracellular polysaccharide (EPS) content increases, enhancing pathogenicity. Therefore, this study aimed to evaluate the antibacterial efficacy of cold atmospheric plasma (CAP) against oral microcosm biofilms and the influence of biofilm maturity on treatment. Oral microcosm biofilms were cultured on hydroxyapatite disks for 2 and 6 days. Based on the treatment and biofilm maturity, these were subsequently allocated into six groups (N = 19 each): Groups 1 and 2 were incubated with distilled water for 1 min; Groups 3 and 4 were treated with CAP for 2 min, and Groups 5 and 6 were treated with 0.12% chlorhexidine gluconate for 1 min. Groups 1, 3, and 5 represent 2-day biofilms, and Groups 2, 4, and 6 represent 6-day biofilms. Treatments were repeated daily for 5 days. Antibacterial efficacy was analyzed by measuring oral biofilms’ red fluorescence intensity (RatioR/G) and quantifying EPS content and bacterial viability. The RatioR/G was 1.089-fold and 1.104-fold higher in Groups 4 and 6 than in Groups 3 and 5 following antibacterial treatment, respectively (p < 0.001). EPS content increased by 1.71-fold in Group 6 than in Group 5 (p < 0.001). Bacterial survival rate was the lowest in Group 3 (p = 0.005). These findings underscore the relevance of CAP treatment in maintaining antibacterial efficacy regardless of the biofilm development stage, highlighting its potential utility in oral care.

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“…for wound healing [1], dentistry [2] and in anticancer therapy [3,4]. Since the 1990s, a novel plasma sterilization method was used for cleaning bio-contaminated surfaces.…”

Section: Introductionmentioning

confidence: 99%

A grounded electrode beneath dielectric targets, including cancer cells, enhances the impact of cold atmospheric plasma jet

Schweigert

Zakrevsky

Milakhina

et al. 2022

Plasma Phys. Control. Fusion

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The eﬀect of placing a grounded substrate beneath the dielectric target or cancer cells during exposure to the cold atmospheric plasma jet is studied in the experiments and in ﬂuid model simulations for the discharge parameters typical for the medical applications. It is shown that the dynamics of streamers generated in each positive cycle of ac voltage depends on the grounded substrate position. The streamers approach the target more often if the grounded substrate is beneath the target, that provides more intensive plasma-target interaction. In this case, the measured spectrum of plasma jet emission near the target demonstrates much higher intensity compared to an electrically isolated target case. The calculated and measured discharge currents with time demonstrate a mismatch of frequencies of the ac voltage and current over the target. The eﬀect of relocation of the grounded substrate on the viability of plasma-irradiated cancer cells is analyzed. The viability of A431 human skin carcinoma and MX7 mouse rhabdomyosarcoma cells treated by cold atmospheric plasma jet is measured with MTT assay 24 hours after. The results show an enhanced suppression of the cell viability with using the grounded substrate for both cell lines. Achieving eﬀective death of tumor cells with a shorter irradiation time can be considered an advantage of using a grounded electrode.

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Nonthermal plasma in dentistry: An update

Cited by 29 publications

References 37 publications

Cold Atmospheric Plasma Medicine: Applications, Challenges, and Opportunities for Predictive Control

Cold Atmospheric Plasma Medicine: Applications, Challenges, and Opportunities for Predictive Control

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

A grounded electrode beneath dielectric targets, including cancer cells, enhances the impact of cold atmospheric plasma jet

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