Nontuberculous mycobacteria in clinical samples with negative acid-fast bacilli

Hernández-Solís, Alejandro; Cícero-Sabido, Raúl; González-Villa, Maribel; Martínez-Rivera, Isaura Isabel; Mandujano-Martínez, América Del Pilar; Torres-Mazadiego, Benita Pilar; Wong-Arámbula, Claudia; Rodríguez-Maldonado, Abril Paulina; Hernández-Rivas, Lucía; González-González, Heleodora; Ramírez–González, José Ernesto

doi:10.4103/ijmy.ijmy_160_17

Cited by 8 publications

(9 citation statements)

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“…It is noteworthy that 16% of the analyzed isolates corresponded to NTM. This is an important percentage although lower than those previously reported for Mexico City (30.8%) (Hernández-Solís et al, 2017) and Northern India (27.4%) (Maurya et al, 2015). As in the above mentioned reports, we found M. avium, M. fortuitum and M. intracellulare as the most frequent infecting species.…”

Section: Discussionsupporting

confidence: 63%

Genetic Diversity and Drug Resistance of Mycobacterium tuberculosis Complex Isolates and Nontuberculous Mycobacteria Identification from Presumptive Tuberculosis Cases in Oaxaca, Mexico

Nakamura-López

Daniel

Monica

et al. 2020

American Journal of Infectious Diseases

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Tuberculosis (TB) is a re-emerging health problem worldwide. In Mexico, information about genetic diversity and drug resistance of Mycobacterium Tuberculosis complex (MTBc) is limited. Samples from 463 Presumptive TB cases were tested for TB by smear, culture and PCR, from which 19.2% were identify as MTBc and 16% as nontuberculous mycobacteria. MTBc isolates were characterized by Large Sequence Polymorphisms (LSPs), spoligotyping and MIRU-VNTR 24 loci typing methods. Clade designations showed 10 sub-lineages: Haarlem (35.7%), EAI (26.2%), LAM (9.5%), Ghana (4.8%), X (2.4%), New-1 (2.4%), H37Rv-like (7.1%), M. bovis (2.4%), S (7.1%), Uganda I (2.4%). The finding of EAI as one of the principal genotypes may be associated with high migration rates. Drug resistance was found in 35.71% of the isolates: 14.2% were multidrug-resistant (MDR-TB), 14.2% mono-resistant and 7.14% poly-resistant. This study provides the first description of genetic diversity and drug resistance profile of MTBc in Oaxaca, Mexico.

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Section: Discussionsupporting

confidence: 63%

Genetic Diversity and Drug Resistance of Mycobacterium tuberculosis Complex Isolates and Nontuberculous Mycobacteria Identification from Presumptive Tuberculosis Cases in Oaxaca, Mexico

Nakamura-López

Daniel

Monica

et al. 2020

American Journal of Infectious Diseases

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“…30 NTM were found in 1.73% of DRTB suspects and in 0.52% TB suspected samples in our study. Varying positivity has been reported for NTM worldwide in TB suspects: 30.8% from Mexico, 31 34.8% from Iran in MDR TB suspects, 32 17.6% from Delhi, India, 4 and 0.77% from North India in TB suspects. 28 These findings highlight the necessity of identification and speciation of NTM so as to initiate appropriate treatment.…”

Section: Discussionmentioning

confidence: 98%

Profile of Nontuberculous Mycobacteria in Patients Suspected of Tuberculosis and Drug-Resistant Tuberculosis

et al. 2020

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Objective Infections due to nontuberculous mycobacteria (NTM) is increasing globally and may present as drug-resistant tuberculosis (DRTB). In India, data on NTM prevalence and species diversity is limited. Present study was conducted to detect the prevalence and profile of NTM among patients suspected of DRTB using paraffin slide culture (PSC)and mycobacteria growth indicator tube (MGIT) culture methods for isolation of NTM. Material and Method A total of 2,938 samples suspected of TB/DRTB were cultured on PSC and MGIT960. Species identification of mycobacterial isolate was done by sequencing of 16s ribosomal RNA gene. Result Among 2938 samples, 35 (1.19%) were found positive for NTM by PSC and 9 (0.30%) were found positive by MGIT. The diversity of NTM species was high (13 species). Out of 35 NTM isolates by PSC, maximum 34.29% (12) isolates were found to be Mycobacterium fortuitum, followed by 11.43% (4) Mycobacterium abscessus and Mycobacterium chelonae, and 42.85% (15) were other species viz. 8.57% (3) were Mycobacterium intracellulare and Mycobacterium kansasii, 5.71% (2) were Mycobacterium peregrinum, and 2.85% (1) were Mycobacterium flavescens, Mycobacterium farcinogenes, Mycobacterium moriokanese, Mycobacterium wolinskyi, Mycobacterium simiae, Mycobacterium goodii, and Mycobacterium terrae each. Coinfection of Mycobacterium tuberculosis(MTB) and NTM was found in 60% (21) samples. Conclusion Prevalence of NTM was low among multidrug resistant tuberculosis/TB suspected patients, similar to other studies done in India. PSC was found better than MGIT for the isolation of NTM, though poor separation of NTM and MTB on subculture may have led to false negativity in cases of coinfection. About 13 species were isolated; M. fortuitum was the most common of all. Since coinfection of NTM and TB can also occur, samples of patients suspected of NTM should be cultured on PSC even if positive for MTB.

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“…Haemolysed or insufficient samples were excluded. Samples of patients diagnosed with nontuberculous mycobacteria [9] were also excluded. Additionally, 30 samples from healthy donors (45 ± 10 years) with no personal or family history of tuberculosis were studied.…”

Section: Populations Of Studymentioning

confidence: 99%

Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures

Cázares-Sosa

Hernández-Solís

Andrés-Dionicio

et al. 2019

J Infect Dev Ctries

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Introduction: The sputum smear or the culture are the definitive diagnosis of pulmonary tuberculosis. Only a fraction of clinical patients are culture-confirmed. Methodology: A total of 24 clinical cases (40 ± 14 years old) with positive smear and negative co-morbidity were studied. Cases were selected from 600 patients who attended the pneumology service over two years. A sputum sample was cultured in Löwenstein-Jensen medium with consequent amplification of the rrnA V2 promoter, the differentiation region 4, and the IS6110 insertion sequence of Mycobacterium tuberculosis. After the culture result, the patients were divided into negative (n = 14) or positive (n = 10) culture groups. In addition, 30 samples from healthy donors (45 ± 10 years) were studied. The numbers of CD4, CD8 and CD19 lymphocytes were determined by flow cytometry. Levels of IgA and IgG to M. tuberculosis were measured by ELISA. Results: IgG and IgA levels were detected in patients with positive culture, while only IgA was found in patients with negative cultures. The lymphocyte populations in the two groups were similar. The presence of a pleural apical cap was found more frequently in patients with negative- (57%) than with positive cultures (10%). Conclusions: The isotype profile in patients with positive cultures was both IgA and IgG positive, while in patients with negative culture, only IgA was found. The results will contribute to improve the diagnostic algorithm and appropriate treatment of patients with clinical tuberculosis. Further studies are needed to determine if this profile is predictive of the outcome of isolation.

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Nontuberculous mycobacteria in clinical samples with negative acid-fast bacilli

Cited by 8 publications

References 1 publication

Genetic Diversity and Drug Resistance of Mycobacterium tuberculosis Complex Isolates and Nontuberculous Mycobacteria Identification from Presumptive Tuberculosis Cases in Oaxaca, Mexico

Genetic Diversity and Drug Resistance of Mycobacterium tuberculosis Complex Isolates and Nontuberculous Mycobacteria Identification from Presumptive Tuberculosis Cases in Oaxaca, Mexico

Profile of Nontuberculous Mycobacteria in Patients Suspected of Tuberculosis and Drug-Resistant Tuberculosis

Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures

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