Nonuniform field gel electrophoresis

Dennison, Christopher; Lindner, W.; Phillips, N. Christopher

doi:10.1016/0003-2697(82)90311-6

Cited by 18 publications

(15 citation statements)

References 5 publications

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“…2-4 demonstrate that agreement between numerical simulations and the approximate solutions (8) and (10) is very good. This allows the use of the analytical approximations to estimate the maximum resolution for given mobility values, the time at which maximum resolution occurs, and the peak capacity of the FGE method.…”

Section: Numerical Simulationsmentioning

confidence: 76%

“…The FGE approach is similar to the method of Dennison et al [8], except that the gradient field in that work was produced by a separation channel of changing cross-sectional area. With a changing cross-section, diffusion of analytes in the plane transverse to the axis of the separation channel counteracts the concentrating effect of the electric field gradient.…”

Section: General Aspectsmentioning

confidence: 99%

“…Alternatively, the external medium may be a porous material in which the separation channel is fabricated as a narrow hole [9]. The advantage of a uniform crosssection over the nonuniform channel of [8] is that transverse diffusion cannot occur. Other related techniques include the pulsed-field gradient electrophoresis method [10], which also employs an electric field gradient to achieve separation, but differs from the present method in that the operating principle is based on reorientation of molecules in a periodically changing field.…”

Section: General Aspectsmentioning

confidence: 99%

See 2 more Smart Citations

Field gradient electrophoresis

Warnick¹,

Francom²,

Humble³

et al. 2005

Electrophoresis

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The class of equilibrium gradient methods utilizes the opposition of two forces, at least one of which changes in magnitude with position, to separate and concentrate analytes. The drawback of many methods of this type is that the production of two opposing forces requires in comparison to standard methods, such as capillary electrophoresis, a relatively complex apparatus. In addition, for techniques such as electric field gradient focusing, hydrodynamic flow leads to Taylor dispersion, which limits the attainable concentration factor. We propose a new method, gradient field electrophoresis, which achieves analyte separation and focusing with only one spatially varying force, an electric field gradient. A model for the method is developed and used to analyze peak capacity. Experimental results for a protein (R-phycoerythrin) are given and compared to the model.

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Section: Numerical Simulationsmentioning

confidence: 76%

Section: General Aspectsmentioning

confidence: 99%

Section: General Aspectsmentioning

confidence: 99%

See 1 more Smart Citation

Field gradient electrophoresis

Warnick¹,

Francom²,

Humble³

et al. 2005

Electrophoresis

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show abstract

“…On the other hand, when the injection width is small, then straight channels gave the best resolutions. Dennison et al (1982) found that (macroscopically) electrophoretic resolutions are higher in conical/wedge shaped polyacrylamide gels than the regular gels. Protein transport in pores of different sizes have also been studied in membrane-based separations (Yu et al, 2003).…”

Section: Introductionmentioning

confidence: 95%

Choosing the Optimal Gel Morphology in Electrophoresis Separation by a Differential Evolution Approach (Dea)

Simhadri

Arce

Stretz

2016

Braz. J. Chem. Eng.

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-This paper introduces an effective optimization approach to investigate the morphological effects of nanocomposite gel electrophoresis and operational parameters (see below) by integrating the numerical simulations based on finite element method and population-based search algorithm such as differential evolution. Simulations are performed to study the solute transport by Convection-Diffusion-Electromigration in a microvoid with axially varying cross-section. Morphological parameters such as channel shape and size, as well as operational parameters such as pressure gradient in the axial direction, and electric field in the orthogonal direction were considered and found to have considerable effects on the separation resolution in electrophoresis. Key observations on the most favorable hydrogel morphology for an efficient electrophoresis separation are presented.

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“…Several research groups have reported on the use of nonuniform electric fields to improve electrophoretic separations. Dennison et al [2] used diverging conical/wedge-shaped polyacrylamide gels and found that at equivalent protein loading, the resolving power of diverging gels was greater than uniform gels. Boncinelli et al [3] and Olsson et al [4] used diverging wedge agarose gels to improve the resolution of DNA molecules.…”

Section: Introductionmentioning

confidence: 99%

Peak compression and resolution for electrophoretic separations in diverging microchannels

Ross¹,

Ivory²,

Locascio³

et al. 2004

Electrophoresis

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We report the results of experiments and simulations on electrokinetic flow in diverging microchannels (with cross-sectional area that increases with distance along the channel). Because of conservation of mass and charge, the velocity of an analyte in the channel decreases as the channel cross-section increases. Consequently, the leading edge of a band of sample moves more slowly than the trailing edge and the sample band is compressed. Sample peak widths, rather than increasing diffusively with time, can then be controlled by the geometry of the channel and can even be made to decrease with time. We consider the possibility of using this peak compression effect to improve the resolution of electrophoretic separations. Our results indicate that for typical separations that are dispersion limited, this peak compression effect is more than offset by the decreased distance between peaks, and the separation resolution in diverging channels is worse than that found for straight channels at the same applied voltage. For separations in very short channels or at very high field strengths, however, when the separation efficiency is injection limited, the peak compression effect is dominant and diverging channels can then be used to achieve improved separation resolution.

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Nonuniform field gel electrophoresis

Cited by 18 publications

References 5 publications

Field gradient electrophoresis

Field gradient electrophoresis

Choosing the Optimal Gel Morphology in Electrophoresis Separation by a Differential Evolution Approach (Dea)

Peak compression and resolution for electrophoretic separations in diverging microchannels

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