2020
DOI: 10.1073/pnas.1919607117
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Nonuniform growth and surface friction determine bacterial biofilm morphology on soft substrates

Abstract: During development, organisms acquire three-dimensional shapes with important physiological consequences. While the basic mechanisms underlying morphogenesis are known in eukaryotes, it is often difficult to manipulate them in vivo. To circumvent this issue, here we present a study of developing Vibrio cholerae biofilms grown on agar substrates in which the spatiotemporal morphological patterns were altered by varying the agar concentration. Expanding biofilms are initially flat, but later experience a mechani… Show more

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Cited by 116 publications
(120 citation statements)
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“…Finite Element Simulation of V. cholerae Pellicles. We use the previously described framework of elastic growth (10,11,18) to model the primary wrinkling instability of V. cholerae pellicles. In brief, we use the deformation gradient tensor F = ∂x=∂X to describe the local shape change of a 2D thin film, in which x = [x,z] T and X = [X,Z] T denote the current coordinates and the material coordinates, respectively.…”
Section: Mathematical Modeling Of the Primary Wavelength For A Heterogeneousmentioning
confidence: 99%
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“…Finite Element Simulation of V. cholerae Pellicles. We use the previously described framework of elastic growth (10,11,18) to model the primary wrinkling instability of V. cholerae pellicles. In brief, we use the deformation gradient tensor F = ∂x=∂X to describe the local shape change of a 2D thin film, in which x = [x,z] T and X = [X,Z] T denote the current coordinates and the material coordinates, respectively.…”
Section: Mathematical Modeling Of the Primary Wavelength For A Heterogeneousmentioning
confidence: 99%
“…These multicellular communities are crucial in contexts such as medical infections and industrial biofouling because cells in biofilms and pellicles display enhanced resilience to antibiotics, immune clearance, and physical perturbations compared to their isogenic planktonic counterparts (7)(8)(9). Analogous to eukaryotic systems, bacterial biofilms and pellicles develop striking macroscopic morphologies including wrinkles and delaminations that are driven by combined biological, materialphysics, and mechanical determinants (10)(11)(12)(13)(14). Studies of biofilms at the single-cell level show the emergence of internal cell ordering (15)(16)(17) and collective cellular flow (18).…”
mentioning
confidence: 99%
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“…When a bacteria makes contact with a surface, it initiates a program of gene expression that promotes colonization and biofilm formation ( 1, 2 ). As the biofilm grows, it absorbs nutrients from its environment and generates internal forces that allow the biofilm to expand and move against its substrate ( 3-6 ). Biofilm growth thus depends on chemical signals but also mechanical interactions with its environment.…”
Section: Introductionmentioning
confidence: 99%
“…Despite their ancient evolutionary origins, bacteria can exhibit complex competition patterns and morphological, especially when they live in communities called biofilms [1,2]. Biofilms are surface-associated bacterial communities encapsulated by a self-produced extracellular matrix, and a predominant growth mode of bacteria in nature [3][4][5]. Biofilms are involved in aspects many of our lives, such as medical care [6], effluents purifying [7], biofuel cells [8].…”
Section: Introductionmentioning
confidence: 99%