Unextendible product basis (UPB), a widely used tool in quantum information, exhibits nonlocality which is the powerful resource for quantum information processing. In this work we extend the definitions of nonlocality and genuine nonlocality from states to operators. We also extend UPB to the notions of unextendible product operator basis, unextendible product unitary operator basis (UPUOB) and strongly UPUOB. We construct their examples, and show the nonlocality of some strongly UPUOBs under local operations and classical communications. As an application, we distinguish the two-dimensional strongly UPUOB which only consumes three ebits of entanglement.