Nonviral transfection of distinct types of human dendritic cells: high-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells

High-level transgene expression in primary human T lymphocytes and adult bone marrow CD34+ cells via electroporation-mediated gene delivery

“…The level of CD34 expression was determined simultaneously with EGFP fluorescence 24 h after transfection at the indicated time-point by immunophenotypic analysis, as described previously. 16 Briefly, 5 × 10 5 transfected cells were washed once in PBS supplemented with 1% FCS and stained for 30 min at 4°C with 1 g of monoclonal antibody in 150 l of PBS containing 1% BSA and 0.1% azide and analyzed on a FACScan flow cytometer. In order to allow combination with EGFP analysis, a phycoerythrin (PE)-conjugated monoclonal CD34 antibody (Becton Dickinson) was used.…”

“…Recently, we have demonstrated efficient transient transfection by electroporation in primary CD34 + progenitor-derived dendritic cells (DC) and Langerhans cells (LC). 16 Here, we want to extend our electroporation protocol to transfect HPC and primary T lymphocytes. Recently, Satoh et al 17 demonstrated that peripheral blood CD34 + HPC could be transfected by electroporation by EBV-based vectors.…”

Poxvirus as a vector to transduce human dendritic cells for immunotherapy: abortive infection but reduced APC function

“…[9][10][11] Some recombinant viral vectors have a high transduction efficiency and are more likely to generate a strong and sustained CD4 + helper cell and a cytotoxic response against tumors. So far, adenovirus and avipoxvirus vectors have been described as efficiently transducing DC.…”

Plasmid DNA adsorbed onto cationic microparticles mediates target gene expression and antigen presentation by dendritic cells

“…17 While progress has been made by the use of electroporation, the efficiency of DC transfection is extremely low and results in substantial loss of cell viability. 18 To date, no purely chemical method has been shown to be effective. To facilitate transfection of DC and recombinant gene expression, DNA must be delivered to the nucleus, and overcome the initial barriers of uptake and endosomal degradation.…”