Nonvolatile Chemical Cues Affect Host-Plant Ranking by Gravid Polygonia c-album Females

Mozūraitis, Raimondas; Murtazina, Rushana; Nylin, Sören; Borg‐Karlson, Anna‐Karin

doi:10.5560/znc.2012.67c0093

Cited by 2 publications

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“…It has been shown that methanol extracts and fractions with the most polar phytochemicals stimulates egg laying in comma butterflies (Mozūraitis et al., ). Based on that knowledge, around 4 000 leaf equivalents of hop foliage were extracted in 3 l of methanol (99.9% pure; Carlo Erba Reactifs, Valle de Reuil, France) for 5 days.…”

Section: Methodsmentioning

confidence: 99%

Volatiles released from foliar extract of host plant enhance landing rates of gravid Polygonia c‐album females, but do not stimulate oviposition

Mozūraitis

Radžiūtė

Apšegaitė

et al. 2016

Entomologia Exp Applicata

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For a long time the role of volatile cues for the search of host-plants was underestimated in 2 the day-active butterflies compared to the closely related nocturnal moths, but it is now 3 known that olfactory cues play an important role. The simultaneous acquiring of information 4 from several senses during host-plant search, recognition and acceptance for oviposition 5 requires appropriate design of experiments and sample preparation in order to investigate the 6 effect of a selected type of cues while ensuring that the contribution of other sensory 7 modalities will remain uniform. The goal of this study was to investigate the effect of host- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 design of experiments and sample preparation in order to manipulate and investigate the 10 effect of a selected type of cues while ensuring that the contribution of other sensory 11 modalities will remain uniform (Städler, 2002). 12In the day-active butterflies the role of olfactory cues for host search have long been 13 underestimated, in particular in comparison to the their relatives, the nocturnal moths.14 However, it has recently been shown that volatile compounds also play an important role in 15 attracting ovipositing butterfly females (e.g. Ikeura et al. 2010; Schäpers et al. 2015) and 16 moreover that the brains of females seem to be adapted to distinguishing host odours from 17 other odours (Carlsson et al., 2011 corresponding to 500 leaf equivalents was taken and stored at -14 °C. Methanol was removed 10 from the remaining extract and an aliquot of the concentrate, after being dispersed in 150 ml 11 of Millipore purity water, was extracted successively twice with 150 ml of chloroform 12 (99.9% Carlo Erba Reactifs-SDS, France) followed by 150 ml of isobutanol (99+% Alfa Sampling of volatiles 23Two leaf equivalents of extract and 100 ng of pentadecane as internal standard were added on 24 10 cm 2 of filter paper and when methanol was used as a solvent it was allowed to evaporate 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 F o r P e e r R e v i e w 7 hexane (1mg/ml) as control stimuli was used to test the sensitiveness of antennae. Three 1 antennae from three females were each used to test the EAG activity of the plant extract. 2The qualitative analyses were performed using a Varian 3400 gas chromatograph coupled 3 with a Finnigan SSQ 7000 mass spectrometer (Termo-Finnigan, San Jose, CA, USA). The 4 GC was equipped with the same type of capillary column and operated under the same 5 condition as described in the EAG experiment except that Helium was used as the carrier gas 6 with an inlet pressure of 70 kPa. Electron ionization mass spectra were dete...

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Section: Methodsmentioning

confidence: 99%