Nonwoven Carboxylated Agarose-Based Fiber Meshes with Antimicrobial Properties

Forget, Aurélien; Arya, Neha; Randriantsilefisoa, Rotsiniaina; Miessmer, Florian; Buck, Marion; Ahmadi, Vincent; Jonas, Daniel E; Blencowe, Anton; Shastri, V. Prasad

doi:10.1021/acs.biomac.6b01401

Cited by 37 publications

(22 citation statements)

References 38 publications

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“…Such structured 3D environments could allow the spatial guidance of cell differentiation by patterned mechanical cues within a hydrogel environment and enable the incorporation of mechanobiology paradigms in the direct 3DP of cells. Furthermore, our study extends the already broad utility of carboxylated agarose in biomedical applications which include synthetic 3D cell culture matrix, [2] nonwoven antimicrobial wound dressing, [39] and antimicrobial hydrogel [40] to bioinks for cell printing.…”

Section: −1mentioning

confidence: 68%

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Forget

Blaeser

Miessmer

et al. 2017

Adv Healthcare Materials

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COMMUNICATION (1 of 7)© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Mechanically Tunable Bioink for 3D Bioprinting of Human CellsAurelien Forget, Andreas Blaeser, Florian Miessmer, Marius Köpf, Daniela F. Duarte Campos, Nicolas H. Voelcker, Anton Blencowe, Horst Fischer,* and V. Prasad Shastri* DOI: 10.1002/adhm.201700255 medium-the bioink. [5] The former approach offers the advantage that the 3D scaffold does not have to be fabricated under cytocompatible conditions. Therefore, a broader range of materials can be employed, for example, thermoplasts, such as poly(caprolactone), [6] and additionally, other alternative scaffold fabrication techniques, such as electrospinning, or pressurized gyration can be combined with subsequent functionalization of the scaffold with proteins. [7] In contrast, the latter approach, that is, direct fabrication of cellloaded constructs by hydrogel molding or 3D printing, places high demands on the cytocompatibility of the material and the fabrication process and the resolution is limited by the size of the extrusion nozzle utilized in the fabrication process. [8] However, the advantage of direct fabrication techniques, especially of 3D bioprinting, is its ability to generate constructs with spatially defined cell and material composition. Moreover, biomaterials that are conventionally used in cell culture such as alginate, [9] Pluronic, [10] gelatin, [11] nanocellulose, [12] self-assembling peptides, [13] and agarose [14] are highly advantageous for direct cell printing as they are soluble in water and hence can be formulated as a cell carrier. There has been extensive effort to build on and improve the properties of watersoluble polymers as bioinks. [15,16] For example, to overcome the limitation of the solubilization of Pluronic and its limited diversity of mechanical properties, blending of Pluronic with alginate [17] and crosslinking using acrylate-modified Pluronic have been explored. [10] Notwithstanding these advances that utilize chemical crosslinking to control the mechanical properties of the bioinks, controlling the shear behavior and mechanical This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels can be tuned over several orders of magnitudes (5-230 Pa) while ensuring almost no change to the shear viscosity (10-17 mPa) of the bioink solution; thus enabling the fabrication of 3D structures made of different mechanical domains under identical printing parameters and low nozzle shear stress. Human mesenchymal stem cells printed using CA as a bioink show significantly higher survival (95%) in comparison to when printed using native agarose (62%), a commonly used thermogelling hydrogel for 3D-bioprinting applications. This work paves the way toward the printing of complex tissue-like structures composed of a range of mechanically discrete microdomains that could potent...

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Section: −1mentioning

confidence: 68%

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Forget

Blaeser

Miessmer

et al. 2017

Adv Healthcare Materials

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“…Recent work illustrated how MSCs could be printed using an agarose-based bioink [136]. While some studies have explored electrospinning agarose fibers, these materials have not been evaluated in combination with stem cells [137]. In summary, agarose scaffolds and their different formulations provide a versatile platform for tissue engineering.…”

Section: Agarosementioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

114

113

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Combining stem cells with biomaterial scaffolds serves as a promising strategy for engineering tissues for both in vitro and in vivo applications. This updated review details commonly used biomaterial scaffolds for engineering tissues from stem cells. We first define the different types of stem cells and their relevant properties and commonly used scaffold formulations. Next, we discuss natural and synthetic scaffold materials typically used when engineering tissues, along with their associated advantages and drawbacks and gives examples of target applications. New approaches to engineering tissues, such as 3D bioprinting, are described as they provide exciting opportunities for future work along with current challenges that must be addressed. Thus, this review provides an overview of the available biomaterials for directing stem cell differentiation as a means of producing replacements for diseased or damaged tissues.

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“…Their great water solubility, biocompatibility, biodegradability and the simplicity of furnishing chemical medications in water media make gum hydrogels and their electrospun membranes eminently attractive polymers in the synthesis of scaffolds for applications in tissue engineering. The functionalization of various natural polymeric materials including gums, mucilage and their amended hydrogel formulas for the development of drug delivery systems has also been highlighted ( Das and Pal, 2015;De France et al, 2017;Forget et al, 2016;Liu et al, 2017;Mano et al, 2007;Rana et al, 2011;Shukla et al, 2016;Silva et al, 2017;Sridhar et al, 2015;Woehl et al, 2014).…”

Section: Biomedical Applications Of the Gum Matrix-metal/metal Oxide mentioning

confidence: 99%

Tree gum-based renewable materials: Sustainable applications in nanotechnology, biomedical and environmental fields

Padil

Wacławek

Černík

et al. 2018

Biotechnology Advances

128

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The prospective uses of tree gum polysaccharides and their nanostructures in various aspects of food, water, energy, biotechnology, environment and medicine industries, have garnered a great deal of attention recently. In addition to extensive applications of tree gums in food, there are substantial non-food applications of these commercial gums, which have gained widespread attention due to their availability, structural diversity and remarkable properties as 'green' bio-based renewable materials. Tree gums are obtainable as natural polysaccharides from various tree genera possessing exceptional properties, including their renewable, biocompatible, biodegradable, and non-toxic nature and their ability to undergo easy chemical modifications. This review focuses on non-food applications of several important commercially available gums (arabic, karaya, tragacanth, ghatti and kondagogu) for the greener synthesis and stabilization of metal/metal oxide NPs, production of electrospun fibers, environmental bioremediation, bio-catalysis, biosensors, coordination complexes of metal-hydrogels, and for antimicrobial and biomedical applications. Furthermore, polysaccharides acquired from botanical, seaweed, animal, and microbial origins are briefly compared with the characteristics of tree gum exudates.

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Nonwoven Carboxylated Agarose-Based Fiber Meshes with Antimicrobial Properties

Cited by 37 publications

References 38 publications

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Tree gum-based renewable materials: Sustainable applications in nanotechnology, biomedical and environmental fields

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