Nonylphenol-induced thymocyte apoptosis involved caspase-3 activation and mitochondrial depolarization

Yao, Genhong; Yang, Lingsong; Hu, Yali; Liang, Jun; Liang, Junfeng; Hou, Yayi

doi:10.1016/j.molimm.2005.06.031

Cited by 56 publications

(40 citation statements)

References 36 publications

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“…A body of evidence from mainly in vitro studies, however, has demonstrated the estrogenicity (Soto et al, 1991 ;Vivacqua et al, 2003 ;White et al, 1994 ), cell proliferative-inducing (Masuno et al, 2003 ;Miura et al, 2005 ;Soto et al, 1991 ) and caspase-dependent death-inducing (Kudo et al, 2004 ;Yao et al, 2006 ) effects of weak estrogenic chemicals such as alkylphenolic compounds (e.g., nonylphenol and octylphenol). Nonylphenol production, which in the United States was 147 million pounds in 1980 and grew to over 230 million pounds in 2000 (Blankenship and Coady, 2005 ), are for diverse uses, ranging from industrial detergents and wetting agents to pesticides, hair dyes, and intravaginal spermicides (Bolt et al, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Stage-Related Increase in the Proportion of Apoptotic Germ Cells and Altered Frequencies of Stages in the Spermatogenic Cycle Following Gestational, Lactational, and Direct Exposure of Male Rats to p-Nonylphenol

McClusky

Jager

Bornman

2006

Toxicological Sciences

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The cumulative effects of environmental toxicants, for example, the alkylphenol, para-nonylphenol (p-NP) are of concern. Our previous study showed that p-NP reduced several testicular morphometric parameters, including sperm counts. The present study reexamined material collected in that study to determine the mechanistic basis of p-NP action on spermatogenic development in the offspring. Seven-day pregnant Sprague-Dawley rats were treated with vehicle or 100 or 250 mg/kg p-NP through gestation, lactation and afterward directly to all male offspring until 10 weeks of age. Both doses of p-NP significantly (P < 0.02) increased the number of germ cells with in situ end-labeled fragmented DNA (TUNEL positive) by 1.9-fold and 1.7-fold, respectively, and specifically in stages XII-XIV and I-III. TUNEL-labeling was, however, selective, and excluded labeling of basal cells with apoptotic morphology. Cleaved caspase-3 immunohistochemistry strongly labeled basal cells (spermatogonia and early spermatocytes) with condensed marginated chromatin but not degenerate germ cells lacking definitive nuclear material found throughout the epithelium. Only the caspase index (ratio of number of caspase positive to number of degenerate cells) of the 100-mg/kg p-NP group was significantly (p < 0.05) threefold greater than controls. Whereas both doses and either 250 or 100 mg/kg treatment alone significantly (p < 0.002) reduced the frequencies (duration) of stages I-III, VII-VIII, and late VIII-IX (spermiating and recently spermiated tubules), respectively, both doses significantly (p < 0.002) increased the frequencies of stages IV-VI and all stages containing late-stage spermatocytes (XII-XIII) and meiotic cell divisions (XIV). Thus, p-NP, an environmentally persistent xenoestrogen, insidiously alters the spermatogenic cycle and spermatogenic process in male offspring.

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Section: Introductionmentioning

confidence: 99%

Stage-Related Increase in the Proportion of Apoptotic Germ Cells and Altered Frequencies of Stages in the Spermatogenic Cycle Following Gestational, Lactational, and Direct Exposure of Male Rats to p-Nonylphenol

McClusky

Jager

Bornman

2006

Toxicological Sciences

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“…These results demonstrate that the violacein induces apoptosis in cancer cells. Apoptosis is necessary for the conservation of tissues homeostasis and important for defense against the diseases and cancers [19,20].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

Santhosh

Palaniswamy

2017

Asian J Pharm Clin Res

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Objectives: This study investigates the in vitro anticancer activity of the violacein extracted from the Chromobacterium vaccinii CV5.Methods: Natural colorants or dyes derived from flora to fauna are believed to be safe because of nontoxic, noncarcinogenic, and biodegradable in nature. There are a number of natural pigments, but only a few are available in sufficient quantities for industrial production. The cytotoxicity activity of pigment was assessed against the cervical (HeLa) and lung cancer (A549) cell lines using the MTT assay and there by potential cytotoxic activity exhibited by the pigment was identified. Results:The result of the pigment shows potent anticancer activity on the two cancer cell lines tested in a concentration dependent manner. The potent anticancer activity was observed with the pigment with IC 50 values of 26 µg/mL on HeLa and 31 µg/mL on A549 cells, respectively. Conclusion:The study is pioneering report for determining the better in vitro anticancer activity of violacein from the novel isolate C. vaccinii CV5.

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“…Cell apoptosis and cell nucleus morphology were detected using the method of hoechst 33258 staining (Araki et al, 1987;Yao et al, 2006). Briefly, the cells were stained by Hoechst 33258 (1 µg/mL) at room temperature in dark for 15 min.…”

Section: Morphological Assessment Of Cell Apoptosis By Hoechst 33258 mentioning

confidence: 99%

Anti-inflammatory and Anticancer Activities of Ethanol Extract of Pendulous Monkshood Root in vitro

Huang¹,

Ren²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Nonylphenol-induced thymocyte apoptosis involved caspase-3 activation and mitochondrial depolarization

Cited by 56 publications

References 36 publications

Stage-Related Increase in the Proportion of Apoptotic Germ Cells and Altered Frequencies of Stages in the Spermatogenic Cycle Following Gestational, Lactational, and Direct Exposure of Male Rats to p-Nonylphenol

Stage-Related Increase in the Proportion of Apoptotic Germ Cells and Altered Frequencies of Stages in the Spermatogenic Cycle Following Gestational, Lactational, and Direct Exposure of Male Rats to p-Nonylphenol

Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

Anti-inflammatory and Anticancer Activities of Ethanol Extract of Pendulous Monkshood Root in vitro

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