Nordamnacanthal potentiates the cytotoxic effects of tamoxifen in human breast cancer cells

Subramani, Tamilselvan; Yeap, Swee Keong; Ho, Wan Yong; Ho, Chai Ling; Osman, Che Puteh; Ismail, Nor Hadiani; Rahman, Nik Mohd Afizan Nik Abdul; Alitheen, Noorjahan Banu

doi:10.3892/ol.2014.2697

Cited by 10 publications

(8 citation statements)

References 21 publications

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“…Here, Artonin E was seen to significantly reduce the viability of MCF-7 cells while increasing its rate of apoptosis in a concentration-dependent manner. The percentage of cells undergoing apoptosis after Artonin E treatment was greater than MCF-7 cells undergoing apoptosis after treatment with Tamoxifen, a standard agent as previously reported by Subramani et al26…”

Section: Discussionsupporting

confidence: 76%

Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells

Etti¹,

Abdullah²,

Hashim³

et al. 2017

DDDT

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Artonin E is a prenylated flavonoid compound isolated from the stem bark of Artocarpus elasticus. This phytochemical has been previously reported to be drug-like with full compliance to Lipinski’s rule of five and good physicochemical properties when compared with 95% of orally available drugs. It has also been shown to possess unique medicinal properties that can be utilized in view of alleviating most human disease conditions. In this study, we investigated the cytotoxic mechanism of Artonin E in MCF-7 breast cancer cells, which has so far not been reported. In this context, Artonin E significantly suppressed the breast cancer cell’s viability while inducing apoptosis in a dose-dependent manner. This apoptosis induction was caspase dependent, and it is mediated mainly through the intrinsic pathway with the elevation of total reactive oxygen species. Gene and protein expression studies revealed significant upregulation of cytochrome c, Bax, caspases 7 and 9, and p21 in Artonin E-treated MCF-7 cells, while MAPK and cyclin D were downregulated. Livin, a member of the inhibitors of apoptosis, whose upregulation has been noted to precede chemotherapeutic resistance and apoptosis evasion was remarkably repressed. In all, Artonin E stood high as a potential agent in the treatment of breast cancer.

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Section: Discussionsupporting

confidence: 76%

Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells

Etti¹,

Abdullah²,

Hashim³

et al. 2017

DDDT

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“…Anthraquinones have immensely contributed to the development of effective anti-cancer drugs [ 20 ]. NDAM has been reported to be cytotoxic to several cancer cell lines based on the MTT assay [ 14 – 16 ]. Herein, NDAM was shown to be cytotoxic to all three breast cancer cell lines, MCF-7, MDA-MB231 and 4T1 cells.…”

Section: Discussionmentioning

confidence: 99%

“…The bioactivities of nordamnacanthal have been reported but are very preliminary. These reports claim that nordamnacanthal possess anti-viral, anti-microbial and cytotoxic effects [ 14 – 16 ]. The toxicity as well as the effectiveness of nordamnacanthal as an anti-cancer agent in an in vivo setting has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L

Abu

Zamberi

Yeap

et al. 2018

BMC Complement Altern Med

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BackgroundMorinda citrifolia L. that was reported with immunomodulating and cytotoxic effects has been traditionally used to treat multiple illnesses including cancer. An anthraquinone derived from fruits of Morinda citrifolia L., nordamnacanthal, is a promising agent possessing several in vitro biological activities. However, the in vivo anti-tumor effects and the safety profile of nordamnacanthal are yet to be evaluated.MethodsIn vitro cytotoxicity of nordamnacanthal was tested using MTT, cell cycle and Annexin V/PI assays on human MCF-7 and MDA-MB231 breast cancer cells. Mice were orally fed with nordamnacanthal daily for 28 days for oral subchronic toxicity study. Then, the in vivo anti-tumor effect was evaluated on 4T1 murine cancer cells-challenged mice. Changes of tumor size and immune parameters were evaluated on the untreated and nordamnacanthal treated mice.ResultsNordamnacanthal was found to possess cytotoxic effects on MDA-MB231, MCF-7 and 4T1 cells in vitro. Moreover, based on the cell cycle and Annexin V results, nordamnacanthal managed to induce cell death in both MDA-MB231 and MCF-7 cells. Additionally, no mortality, signs of toxicity and changes of serum liver profile were observed in nordamnacanthal treated mice in the subchronic toxicity study. Furthermore, 50 mg/kg body weight of nordamncanthal successfully delayed the progression of 4T1 tumors in Balb/C mice after 28 days of treatment. Treatment with nordamnacanthal was also able to increase tumor immunity as evidenced by the immunophenotyping of the spleen and YAC-1 cytotoxicity assays.ConclusionNordamnacanthal managed to inhibit the growth and induce cell death in MDA-MB231 and MCF-7 cell lines in vitro and cease the tumor progression of 4T1 cells in vivo. Overall, nordamnacanthal holds interesting anti-cancer properties that can be further explored.

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“…28 Oxidative stress can cause permanent modification of genetic material 29 which represents the first step involved in mutagenesis, carcinogenesis and various disorders such as Alzheimer, Hungtinton's disease, diabetes, and Parkinson. 30,31 In the previous report, nordamnacanthal was found to enhance cytotoxic effect of tamoxifen in treating human 32 Damnacanthal 6 enhanced the expression of p21 and caspase-7 subsequently increased apoptosis in human breast cancer MCF7 cell. 33 In the present study, only three other major compounds screened for cytotoxic activities using MCF7 and 4T1 cell lines ( Table 5).…”

Section: Anticancer Activitymentioning

confidence: 88%

A REVIEW ON THE CHEMISTRY AND PHARMACOLOGY OF Rennellia elliptica Korth

Osman

Ismail

2017

IJTID

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Rennellia elliptica, popularly dubbed as Malaysian Ginseng, is widely used in traditional medicine among the local Jakun community in Endau-Rompin State Park, Pahang, Malaysia. The decoction of the roots is traditionally taken for treatment of body aches, as postpartum tonic, as aphrodisiac and for the treatment of jaundice. In the effort of searching new botanical drugs and drug candidates from tropical rainforest, the team from this laboratory had conducted a sizeable phytochemical and biological screening program of tropical plant at Endau Rompin State Park, Pahang with the help from the indigenous people. R. elliptica showed strong antiplasmodial activity in vitro with the IC50 value of 4.04µg/mL. The comprehensive study on the root extract of R. elliptica in this laboratory yielded seventeen compounds from four different classes, including 2 new pyranoanthraquinones, one new anthraquinone, eleven known anthraquinones, one lactone triterpenoid, one coumarin and one phenolic acid. The chemical profile of the root extract was established using HPLC and the selected marker compounds were used as external standards and quantified using standard calibration curve. Nordamnacanthal 5, damnacanthal 7, 2-formyl-3-hydroxy-9,10-anthraquinone 6, 2-methyl-3-hydroxy-9,10-anthraquinone 11 and 1,2-dimethoxy-6-methyl-9,10-anthraquinone 3 were determined at 3.57, 10.32, 4.47, 12.18 and 4.09 µg/g, respectively. Owing to the toxicity of dichloromethane, the extraction of the desired marker compounds was attempted using accelerated solvent extraction and soxhlet extraction using ethanol and water at different compositions. R. elliptica root extract and the isolated anthraquinones showed potential antiplasmodial activity, and the active compounds were probed for their mode of action. In addition, the dichloromethane root extract of R. elliptica and the selected anthraquinones were screened for anticancer, antioxidant, and α-glucosidase inhibitory activities as well as toxicity study in vitro. The review summarizes the findings on Rennellia elliptica which includes phytochemistry, toxicity and its biological activities. The chemotaxonomic significance of Rennellia elliptica is also discussed

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Nordamnacanthal potentiates the cytotoxic effects of tamoxifen in human breast cancer cells

Cited by 10 publications

References 21 publications

Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells

Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells

Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L

A REVIEW ON THE CHEMISTRY AND PHARMACOLOGY OF Rennellia elliptica Korth

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