Norepinephrine protects against apoptosis of mesenchymal stem cells induced by high glucose

Kong, Yanan; Cheng, Liuhanghang; Ma, Li; Li, Haihong; Yu, Zhiwen

doi:10.1002/jcp.28686

Cited by 20 publications

(23 citation statements)

References 63 publications

(99 reference statements)

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“…Then, the cells were cultured in HG (25 mmol/L) DMEM for two weeks and then harvested for further analysis. 26 Morroniside was dissolved in phosphate-buffered saline (PBS) at different concentrations (1, 10 and 100 μmol/L) for dose-dependent treatments except for the Counting Kit-8 (CCK-8) assay, where we added a group of 1000 μmol/L morroniside. When the concentration of morroniside is not specifically defined, the concentration was 100 μmol/L.…”

Section: Isolation and Culture Of Rat Bmscsmentioning

confidence: 99%

Morroniside attenuates high glucose–induced BMSC dysfunction by regulating the Glo1/AGE/RAGE axis

et al. 2020

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Objectives High glucose (HG)–mediated bone marrow mesenchymal stem cell (BMSC) dysfunction plays a key role in impaired bone formation induced by type 1 diabetes mellitus (T1DM). Morroniside is an iridoid glycoside derived from the Chinese herb Cornus officinalis, and it has abundant biological activities associated with cell metabolism and tissue regeneration. However, the effects and underlying mechanisms of morroniside on HG‐induced BMSC dysfunction remain poorly understood. Materials and methods Alkaline phosphatase (ALP) staining, ALP activity and Alizarin Red staining were performed to assess the osteogenesis of BMSCs. Quantitative real‐time PCR and Western blot (WB) were used to investigate the osteo‐specific markers, receptor for advanced glycation end product (RAGE) signalling and glyoxalase‐1 (Glo1). Additionally, a T1DM rat model was used to assess the protective effect of morroniside in vivo. Results Morroniside treatment reverses the HG‐impaired osteogenic differentiation of BMSCs in vitro. Morroniside suppressed advanced glycation end product (AGEs) formation and RAGE expression by triggering Glo1. Moreover, the enhanced osteogenesis due to morroniside treatment was partially blocked by the Glo1 inhibitor, BBGCP2. Furthermore, in vivo, morroniside attenuated bone loss and improved bone microarchitecture accompanied by Glo1 upregulation and RAGE downregulation. Conclusions These findings suggest that morroniside attenuates HG‐mediated BMSC dysfunction partly through the inhibition of AGE‐RAGE signalling and activation of Glo1 and may be a potential treatment for diabetic osteoporosis.

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Section: Isolation and Culture Of Rat Bmscsmentioning

confidence: 99%

Morroniside attenuates high glucose–induced BMSC dysfunction by regulating the Glo1/AGE/RAGE axis

et al. 2020

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“…group (3.75±0.54) h and HG-LG group (3.83±0.51) h, F12 group (9.40±0.83) h, HG group (12.67±1 25). h. There was no signi cant difference in the 75% cell adhesion time of LG group and HG-LG group, while the difference between the other each two groups were statistically different (P<0.01).…”

mentioning

confidence: 80%

“…Studies have shown that medium with high glucose concentration within a certain range could effectively improve the proliferation ability of MSCs [23,24]. However, some studies also have shown that medium with high glucose might accelerate cell senescence and apoptosis in seveal pathways [25][26][27]. Yanan Kong et al cultured MSCs with high glucose (33 mmol/L) medium, and found that the apoptosis rate of cells remained low after culturing for 1 day, while the apoptosis rate of cells increased greatly after culturing for 5 days [25].…”

Section: Discussionmentioning

confidence: 99%

“…However, some studies also have shown that medium with high glucose might accelerate cell senescence and apoptosis in seveal pathways [25][26][27]. Yanan Kong et al cultured MSCs with high glucose (33 mmol/L) medium, and found that the apoptosis rate of cells remained low after culturing for 1 day, while the apoptosis rate of cells increased greatly after culturing for 5 days [25]. And they came to the conclusion that cell apoptosis induced by high glucose was closely related to culture time.…”

Section: Discussionmentioning

confidence: 99%

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Comparison and Optimization: Different Medium and a Novel Scheme for Rabbit Bone Marrow Mesenchymal Stem Cells Culture

Cong

Jian-biao

Hui-xiang

et al. 2020

Preprint

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BackgroundRabbit bone marrow mesenchymal stem cells (rBMSCs) are widely used as seed cells for bone tissue engineering, but the effect of different medium on the physiological characteristics of rBMSCs is still not clear and the optimal culture medium for rBMSCs is also uncertain.MethodsRBMSCs were divided into 4 groups: DMEM/F12 (F12) group, DMEM/Low glucose (LG) group, DMEM/High glucose (HG) group, and optimizational culture method group which was cultured with DMEM/High glucose for the first 6 days and then DMEM/Low glucose (HG-LG). The morphology, proliferation ability, stem cell surface markers, multi-directional differentiation potential, adhesion ability and vitality in tissue engineering bone (TEB) were detected and compared. ResultsRBMSCs in the 4 groups shared the same shape, similar surface markers (positive in CD44, CD29, negative in CD45, CD34) and similar osteogenic, adipogenic and chondrogenic directional differentiation capability. For the primary rBMSCs, the proliferation ability of HG group was higher than that of LG group (P<0.05), while F12 group was between the former two. The proliferation ability of the 3rd passage of rBMSCs in the 4 groups was similar. The adhesion ability of the 3rd passage rBMSCs in the 4 groups was listed as follows starting from high to low: LG group and HG-LG group (they had similar ability), F12 group, HG group, and the difference was statistically significant (P<0.05). The vitality of TEB in LG group and HG-LG group was higher than that in F12 group and HG group (P<0.05). ConclusionsThe primary rBMSCs cultured with DMEM/High glucose showed good proliferation ability, while the 3rd rBMSCs cultured with DMEM/Low glucose showed good adhesion ability and vitality in TEB. RBMSCs cultured with DMEM/High glucose for the first 6 days and then DMEM/Low glucose showed good proliferation ability, good adhesion ability and good vitality in TEB, which might provide a novel optimizational scheme for rBMSCs culture.

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“…MSCs were isolated as before [17]. Brie y, bone marrow cells were collected by ushing the femurs and tibias of 4 weeks old mice(n=10).…”

Section: Isolate and Culture Bone-marrow-derived Mscsmentioning

confidence: 99%

Hematopoietic stem cells can differentiate into pericytes and myofibroblast in wound healing, not bone Mesenchymal stem cells

Kong

Cheng

Xuan³

et al. 2020

Preprint

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Background Hematopoietic stem cells(HSCs) and mesenchymal stem cells(MSCs) can participate in wound healing. However, very few studies had shown HSCs and MSCs could arrive to the wound and differentiate into tissues. In this study, we intend to investigate the role of bone marrow HSCs and MSCs in wound healing. Methods We first removed the bone marrow of mice by irradiation. Furthermore, we injected different colours of fluorescent HSCs and MSCs into the tail vein of irradiated mice to reconstruct bone marrow function. We prepared wound models on the back of these mice. In vivo imaging and immunohistochemical staining were used to track the expression of fluorescent protein. Results HSCs and MSCs have been isolated and cultured. HSCs expressed expressed Sca1, not lineage, CD34 or CD48. MSCs expressed expressed CD29 and CD44,not CD34 or CD45. HSCs labeled with green fluorescent protein reached the wound and co-expressed with desmin and α-SMA. MSCs didn’t stay on the wound. Conclusions The results show HSCs in the bone marrow of mice can directly participate in wound healing and differentiate into pericytes and myofibroblasts.

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Norepinephrine protects against apoptosis of mesenchymal stem cells induced by high glucose

Cited by 20 publications

References 63 publications

Morroniside attenuates high glucose–induced BMSC dysfunction by regulating the Glo1/AGE/RAGE axis

Morroniside attenuates high glucose–induced BMSC dysfunction by regulating the Glo1/AGE/RAGE axis

Comparison and Optimization: Different Medium and a Novel Scheme for Rabbit Bone Marrow Mesenchymal Stem Cells Culture

Hematopoietic stem cells can differentiate into pericytes and myofibroblast in wound healing, not bone Mesenchymal stem cells

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