Normal and Neoplastic Maturation of T-lineage Lymphocytes

Weissman, Irving L.; Baird, SM; Gardner, Richard L.; Papaioannou, Virginia E.; Raschke, William C.

doi:10.1101/sqb.1977.041.01.005

Cited by 61 publications

(44 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, our results, obtained with embryos whose age was precisely determined and before blood circulation had started, rule out the possibility that the lymphoid precursor cells detected in the yolk sac were circulating cells that originated elsewhere, which could not be excluded in previous in vivo studies carried out with day 9 or older mouse embryos (22)(23)(24)(25).…”

mentioning

confidence: 67%

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro.

Palacios

Imhof

1993

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro ( ABSTRACTWe have studied both in vitro and in vivo the formation of lymphocyte progenitors before blood circulation (day 9 of gestation) has started in the mouse embryo, and we have determined the tissue where this occurs. The results demonstrate that the yolk sac of embryos at day 8 and day 8.5 of gestation contains precursor cells that can give rise, in vivo and in vito, to mature T and B lymphocytes. No lymphoid precursors were found in the embryo proper at this stage of mouse development. The yolk sac cells with lymphocyte precursor potential are most likely multipotent stem cells rather than cell-lineage-determined T-and/or B-lymphocyte progenitors. The dermed in vitro assays described here that support differentiation of yolk sac stem cells along the T-or B-lymphocyte pathways also may now facilitate the study of the molecular events leading to cell-lineage commitment of lymphocyte progenitors in the mouse embryo.

show abstract

mentioning

confidence: 67%

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro.

Palacios

Imhof

1993

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…We later showed that the thymic outer cortex contains immature cells that differentiate into naive T cells of all T cell subsets (35,46), reaching the medulla and undergoing selection, which in our hands began after the first T cell receptor (TCR) rearrangement of β-chains and then α-chains, with positive selection preceding negative selection at distinct phases of thymic maturation (47)(48)(49)(50)(51)(52)(53)(54)(55). At each stage of maturation the TCR/CD3 complex was upregulated starting with 1 unit in the CD4 lo 8 lo ckit + IL7R + immature blasts, which if positively selected gave rise to CD4 hi 8 lo or CD4 lo 8 hi transitional intermediate (TI) cells with 3 units of TCR expression on the cell surface but went on to become the double positive (DP)-high blasts with 1 unit of TCR expression, which rearranged a second TCR α-chain for another chance at positive selection (55).…”

Section: The Thymus Thymic Maturation B Lymphocyte Development Hommentioning

confidence: 99%

“…The MLV agglutinated ML cells, but not lymphomas induced by other leukemogenic viruses, RadLV, and AKR or Gross LV. Between these viruses they had common gag and pol polyproteins but only differed at env (50,99). So we embarked on a study of what variable receptors on different T lymphoma cells could be env-specific LV-binding entities.…”

Section: Before the Discovery Of Hematopoietic Stem Cells An Unpopulmentioning

confidence: 99%

How One Thing Led to Another

Weissman

2016

Annu. Rev. Immunol.

View full text Add to dashboard Cite

I started research in high school, experimenting on immunological tolerance to transplantation antigens. This led to studies of the thymus as the site of maturation of T cells, which led to the discovery, isolation, and clinical transplantation of purified hematopoietic stem cells (HSCs). The induction of immune tolerance with HSCs has led to isolation of other tissue-specific stem cells for regenerative medicine. Our studies of circulating competing germline stem cells in colonial protochordates led us to document competing HSCs. In human acute myelogenous leukemia we showed that all preleukemic mutations occur in HSCs, and determined their order; the final mutations occur in a multipotent progenitor derived from the preleukemic HSC clone. With these, we discovered that CD47 is an upregulated gene in all human cancers and is a “don't eat me” signal; blocking it with antibodies leads to cancer cell phagocytosis. CD47 is the first known gene common to all cancers and is a target for cancer immunotherapy.

show abstract

“…Moore and Metcalf showed that the precirculation mouse yolk sac contained hematopoietic progenitors that could be read out either by in vitro colony assays (Moore and Metcalf, 1970), or by the generation of day 10 spleen colonies using the method of Till and McCulloch (Becker et al, 1963). In 1977 and 1978 Weissman, Papiouannou and Gardner isolated genetically defined donor blood cells from precirculation or just post-circulation mouse yolk sacs, and transplanted them in utero to the yolk sac cavities of synchronic allogeneic embryos (Weissman et al, 1977;. These mice were chimeric for life in both the myeloerythroid and the lymphoid lineages.…”

Section: Chimeric Analysis Of Spleen Coloniesmentioning

confidence: 99%

The origin and fate of yolk sac hematopoiesis: application of chimera analyses to developmental studies

Ueno

Weissman²

2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

During mammalian development, as exemplified by mice, hematopoietic cells first appear in the yolk sac blood islands, then in the dorsal aorta of the aorta-gonad-mesonephros (AGM) region and the placenta, eventually seeding into liver, spleen and then bone marrow. The formation of hematopoietic stem cells from mesodermal precursors has finished by mid-fetal life. Once established, the hematopoietic system must supply blood cells to host circulation and tissues for the entire life of the animal. Easy access to hematopoietic cells has enabled a vast number of studies over the last several decades, and much is now understood about the different hematopoietic lineages, how they differentiate, and their derivation from immature progenitors. Yet to be elucidated are the following two intriguing questions: do yolk sac and AGM hematopoietic cells arise from a common precursor or from distinct precursor cells?; and what is the lineage relationship between blood and endothelial cells. In this review, we will survey the state of our current knowledge in these areas, and discuss the potential use of multicolor chimera analyses to elucidate unresolved questions.

show abstract

Normal and Neoplastic Maturation of T-lineage Lymphocytes

Cited by 61 publications

References 0 publications

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro.

At day 8-8.5 of mouse development the yolk sac, not the embryo proper, has lymphoid precursor potential in vivo and in vitro.

How One Thing Led to Another

The origin and fate of yolk sac hematopoiesis: application of chimera analyses to developmental studies

Contact Info

Product

Resources

About