Normal and shear stresses influence the spatial distribution of intracellular adhesion molecule-1 expression in human umbilical vein endothelial cells exposed to sudden expansion flow

McKinney, V.Z.; Rinker, Kristina D.; Truskey, George A.

doi:10.1016/j.jbiomech.2005.02.002

Cited by 24 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7). Whether this redistribution to upstream extremities of the ECs is flow dependent is beyond the scope of this study: it is known that ICAM-1 expression can be upregulated in a shear-dependent manner (20,28).…”

Section: Discussionmentioning

confidence: 99%

TNF-α activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions

Sumagin

Sarelius

2006

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

Sumagin, Ronen, and Ingrid H. Sarelius. TNF-␣ activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions. Am J Physiol Heart Circ Physiol 291: H2116 -H2125, 2006. First published June 9, 2006 doi:10.1152/ajpheart.00248.2006.-The observation that leukocyteendothelial cell (EC) interactions are localized to specific regions on the microvessel wall suggests that adhesion molecule distribution is not uniform. We investigated ICAM-1 distribution and leukocyte-EC interactions in blood-perfused microvessels (Ͻ80 m) in cremaster muscle of anesthetized mice, using intravital confocal microscopy and immunofluorescent labeling. Variability of ICAM-1 expression directly determines leukocyte adhesion distribution within the venular microcirculation and contributes to leukocyte rolling in arterioles during inflammation. The number of rolling interactions increased with ICAM-1 intensity (r 2 ϭ 0.69, P Ͻ 0.05), and rolling velocity was lower in regions of higher ICAM-1 intensity. In controls, venular ICAM-1 expression was approximately twofold higher than in arterioles. After TNF-␣ treatment, ICAM-1 expression was significantly increased, 2.8 Ϯ 0.2-fold in arterioles and 1.7 Ϯ 0.2-fold in venules (P Ͻ 0.05).

show abstract

Section: Discussionmentioning

confidence: 99%

TNF-α activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions

Sumagin

Sarelius

2006

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…These conditions largely dictate their inflammatory response in the form of altered-adhesion molecule expression [75,76], cytokine secretion [77], leukocyte recruitment and transmigration, and release of vasoactive compounds by mechanotransduction [78]. Although pro-or atherothrombotic flow conditions have been described by several groups, such studies have largely focused on only one cell type and ignored any cell-specific responses in a more complex milieu [49,79,80].…”

Section: Endothelial Cellsmentioning

confidence: 99%

Biological effects of dynamic shear stress in cardiovascular pathologies and devices

Girdhar

Bluestein

2008

Expert Review of Medical Devices

View full text Add to dashboard Cite

Altered and highly dynamic shear stress conditions have been implicated in endothelial dysfunction leading to cardiovascular disease, and in thromboembolic complications in prosthetic cardiovascular devices. In addition to vascular damage, the pathological flow patterns characterizing cardiovascular pathologies and blood flow in prosthetic devices induce shear activation and damage to blood constituents. Investigation of the specific and accentuated effects of such flow-induced perturbations on individual cell-types in vitro is critical for the optimization of device design, whereby specific design modifications can be made to minimize such perturbations. Such effects are also critical in understanding the development of cardiovascular disease. This review addresses limitations to replicate such dynamic flow conditions in vitro and also introduces the idea of modified in vitro devices, one of which is developed in the authors' laboratory, with dynamic capabilities to investigate the aforementioned effects in greater detail.Keywords cardiovascular disease; cone-plate viscometers; dynamic shear stress; platelet activation; prosthetic heart valves; turbulence Implantable blood recirculation devices, artificial hearts and heart valves, have long been used as life-saving alternatives for people with severe cardiovascular diseases. However, such devices require complex anticoagulation therapy and, as a consequence, are linked to postimplant complications, such as hemorrhage. Despite the anticoagulation therapy, the risk for cardioembolic stroke is not eliminated. Although biocompatibility of these recirculation devices is of utmost importance, optimizing the geometric design of these devices is also critical to avoid flow-induced trauma to blood. Over the years, a definite link between fluid shearstress-induced damage and activation of blood constituents such as platelets and red blood cells, has been established. Irregular flow patterns arising around complex geometries (e.g., the hinge regions of mechanical heart valves [MHV]) have been recently characterized by numerical calculations and noninvasive flow measurements, and are implicated in causing flow-induced thromboembolism (TE). For instance, the Medtronic Parallel™ valve exhibited regions of elevated turbulent stress around the hinges due to its specific geometry, which, in †Author for correspondence: Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, NY 11794-8181, USA, Tel.: +1 631 444 1259, Fax: +1 631 444 6646, danny.bluestein@sunysb.edu. Financial & competing interests disclosure:The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manu...

show abstract

“…The parallel plate flow chamber is a commonly used apparatus to elucidate this relationship. The experimental setup may be sometimes cumbersome and chamber variations due to construction or assembly as well as timedependent changes in pressure or flow rate may affect flow-sensitive cell responses (McKinney et al 2006). The flow chamber described facilitates the application of shear stress to underlying adherent (e.g.…”

Section: Unsteady Wall Shear Stressesmentioning

confidence: 99%

“…The maximal shear force exists outside the ATR right below the inlet and outlet ports of the perfusion chamber. This could lead to undesired effects in these regions with higher shear forces as described by (McKinney et al 2006). As has been stated before, the usage of a peristaltic pump renders the flow within the flow chamber unsteady despite the usage of the damper.…”

Section: Unsteady Wall Shear Stressesmentioning

confidence: 99%

Flow in the well: computational fluid dynamics is essential in flow chamber construction

et al. 2007

View full text Add to dashboard Cite

A perfusion system was developed to generate well defined flow conditions within a well of a standard multidish. Human vein endothelial cells were cultured under flow conditions and cell response was analyzed by microscopy. Endothelial cells became elongated and spindle shaped. As demonstrated by computational fluid dynamics (CFD), cells were cultured under well defined but time varying shear stress conditions. A damper system was introduced which reduced pulsatile flow when using volumetric pumps. The flow and the wall shear stress distribution were analyzed by CFD for the steady and unsteady flow field. Usage of the volumetric pump caused variations of the wall shear stresses despite the controlled fluid environment and introduction of a damper system. Therefore the use of CFD analysis and experimental validation is critical in developing flow chambers and studying cell response to shear stress. The system presented gives an effortless flow chamber setup within a 6-well standard multidish.

show abstract

Normal and shear stresses influence the spatial distribution of intracellular adhesion molecule-1 expression in human umbilical vein endothelial cells exposed to sudden expansion flow

Cited by 24 publications

References 31 publications

TNF-α activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions

TNF-α activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions

Biological effects of dynamic shear stress in cardiovascular pathologies and devices

Flow in the well: computational fluid dynamics is essential in flow chamber construction

Contact Info

Product

Resources

About