Normal cerebral blood vessels under ultrasound in SD rats of different ages

Abstract

The objective of this study was to examine whether ultrasound can examine the development of cerebral vascular structure and cerebral blood flow in Sprague-Dawley (SD) rats by ultrasound in a noninvasive manner, which provides a reference for ultrasound research of SD rats. Thirty-nine SD rats (7-16 days old) were divided into seven groups according to age, and the number of SD rats in each group was, respectively, 7, 17, 1, 3, 2, 8, and 1. Ultrasound was used to detect cerebral blood vessels, cerebrovascular flow velocity, and heart rate in SD rats in the sagittal and coronal positions, and images were obtained in B-mode ultrasound. The cerebral vascular structure of 39 SD rats (7-16 days) was dynamically observed under B-ultrasound. We found that the cerebral vascular structure of the rats aged 7-10 days was clear and detectable. Rats aged 11-16 days of cerebral vascular structures became thinner and undetectable. Quantitative analysis of cerebrovascular flow rate and heart rate in rats found that there was no significant difference in cerebrovascular blood flow rate and heart rate between 7 and 8 days.Ultrasound can also be used in rat animal studies, that is, the cerebral blood flow in rats of different ages can be monitored in real-time by ultrasound in a noninvasive way.

Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling

“…The complicated procedures and long-time consumption of them stand in the way of their wide application on laboratory animals. As a well-established research tool, TCD has been widely used to diagnose newborns HIE (Luo et al, 2022). A contemporary prospective cohort focusing on mild HIE obtained TCD measures shortly after birth and were classified in the first day after birth (Natique et al, 2021).…”

“…The changes of cerebral hemodynamics in rats were detected using TCD (Apogee 5300, Shantou Ultrasonic Instrument Research Institute Co., Ltd., Shantou, China) (Luo et al, 2022). TCD detected cerebral vessels, coronary blood flow velocities and heart rate (HR) in SD rats at 1, 2, 3, and 7 days after modeling in sagittal and coronal sections.…”