Normal cytogenetic values for bone marrow based on studies of bone marrow transplant donors

Kuffel, Daniel G.; Schultz, Cloann G.; Ash, Robert C.; Dewald, Gordon W.

doi:10.1016/0165-4608(91)90233-k

Cited by 18 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A qualitative defect in progenitor cells is also indicated by the presence of clonal and non-clonal karyotypic abnormalities in a percentage of our patients after ASCT; these defects, which have an incidence of 9/31 in our homogeneous series and do not seem to have any impact on prognosis, are very rare in normal bone marrow 28 and were never seen in any of the cases examined before harvesting. A qualitative defect in haematopoietic cells could derive from the freezing process, 29 the pre-transplant conditioning regimen (especially when including TBI) 30 or from the process of outgrowth in a damaged micro-environment.…”

Section: Discussionmentioning

confidence: 95%

Haematopoietic abnormalities after autologous stem cell transplantation in lymphoma patients

Soligo

Deliliers

Servida

et al. 1998

Bone Marrow Transplant

View full text Add to dashboard Cite

Summary:Haematopoietic reconstitution after autologous stem cell transplantation (ASCT) was evaluated at different times in 26 lymphoma patients. All of the patients showed a significant decrease in the number of both committed (CFU-C) and more primitive progenitor cells (LTC-IC). The expansion of bone marrow progenitor cells in a 'stroma-free' long-term liquid culture system supplemented with SCF, IL-3, IL-6 and GM-CSF from 19 transplanted patients was significantly reduced compared to normal controls. The stromal cell compartment, evaluated by means of a CFU-F assay, was also greatly reduced. The number of haematopoietic and stromal cell progenitors was, nevertheless, very similar to their pre-transplant values. Bone marrow histology, which was evaluated at different times after transplant, showed an increase in reticulin fibres, the dilatation of parenchymal sinusoids and some morphological evidence of trilineage dysplasia in 11 patients; however, the same abnormalities were seen in the majority of pretransplant samples. No cytogenetic abnormalities were observed in 15 patients before transplant, but four subsequently developed persistent clonal karyotypic alterations and five showed non-clonal abnormalities that generally disappeared over time. Our data suggest that both the stromal and the haematopoietic compartments are somehow damaged after ASCT for lymphoma; however, these defects generally pre-exist the transplant conditioning regimen and seem to become less pronounced over time. Keywords: bone marrow transplantation; cytogenetics; haematopoietic stem cells; stromal cells High-dose chemo-radiotherapy followed by the infusion of cryo-preserved autologous bone marrow or peripheral blood stem cells has been increasingly used for the treatment of a variety of malignant haematopoietic diseases. 1After the pancytopenic period, the majority of patients recover normal peripheral blood counts and tend to maintain them for many years after the transplant.2,3 However,

show abstract

Section: Discussionmentioning

confidence: 95%

Haematopoietic abnormalities after autologous stem cell transplantation in lymphoma patients

Soligo

Deliliers

Servida

et al. 1998

Bone Marrow Transplant

View full text Add to dashboard Cite

show abstract

“…This falls within the frequencies of 0.5-5% of sporadic chromosome abnormalities in short-term (1-3 days) bone marrow cultures of normal individuals. 30,31 Moreover, the chromosome abnormalities were observed in one cord blood (CB013) sample, only. A mutagenic exposure of the fetus during gestation and/or an inherited chromosome instability syndrome may have contributed to the increased fragility in this sample.…”

Section: Discussionmentioning

confidence: 99%

Culturing human umbilical cord blood: a comparison of mononuclear vs CD34+ selected cells

Fietz

Berdel

Rieder

et al. 1999

Bone Marrow Transplant

View full text Add to dashboard Cite

Summary:We compared UCB mononuclear cells (MNC) with CD34؉ selected cells in a serum-free static culture system. Cell number proliferation of MNCs was inferior to CD34 ؉ selected cells. MNCs, however, showed a substantial increase from 0.94% CD34 ؉ cells on day 0 to 5.8% on day 7, whereas in the CD34؉ selected samples the CD34؉ cell content declined continously from 62.2% on day 0 to 27.7% on day 7. The number of CFU-GM increased during culture of both cell fractions. Here, only the MNCs showed a substantial increase in clonogenicity on day 7 and day 14 to 11.1-and 4.1-fold input, respectively. This expansion of the CD34 ؉ progenitor cell pool in the MNCs fraction was at least in part attributable to T cells, since the physical abrogation of T cells blocked this effect. Refeeding and reseeding of cells on day 7 had stimulating effects especially on the CD34؉ cells, where cell number proliferation increased from 16.3-fold without to 58.1-fold on day 14. Also, we could find sporadic chromosomal aberrations in four of 100 metaphases examined after 7-20 days of ex vivo expansion. The significance of this observation needs to be clarified in a larger series.

show abstract

“…These analyses identify indirectly the karyotype of donor cells. Various types of constitutional abnormalities of karyotype have been reported to be transmitted after allogeneic hematopoietic stem cell transplantation as shown in Table 1 (Graze et al 1977; Becher et al 1986; Kuffel et al 1991; Barquinero et al 1995; Halaburda et al 2000; Moore et al 2005; Manola et al 2006; Ismail et al 2007; Balci et al 2008; Frey et al 2008; Consoli et al 2011). All donors with Down syndrome were identified before stem cell donations as the manifestations of Down syndrome were evident.…”

Section: Discussionmentioning

confidence: 99%

Donor-derived 47, XXY in an unrelated cord blood transplant recipient

et al. 2014

View full text Add to dashboard Cite

A 65-year-old Japanese male with therapy-related myelodysplastic syndrome was admitted for unrelated cord blood transplantation. A cord blood unit from a male donor was obtained from the Japan Cord Blood Bank Network. The patient then received a conditioning regimen consisting of fludarabine, intravenous busulfan, and total body irradiation. Successful engraftment was obtained. The bone marrow examination on day 28 revealed trilineage engraftment, and chimerism analysis by variable number of tandem repeat polymerase chain reaction confirmed complete donor chimerism. At that time, conventional cytogenetics of the bone marrow aspirate showed 20 out of 20 metaphases with the 47, XXY karyotype characteristic of Klinefelter syndrome. Klinefelter syndrome is the most common genetic cause of human male infertility with a reported prevalence of 0.1–0.2% in the general population. In Japan Cord Blood Bank Network, there is no informed consent from parents about the possibility that post-unrelated cord blood transplantation patient evaluation may reveal donor-origin inherited diseases including cytogenetic abnormality. It is desirable to have opportunities in Japan discussing whether parents will be notified of the possibility that post-unrelated cord blood transplantation evaluation may reveal donor-derived illness incidentally.

show abstract

Normal cytogenetic values for bone marrow based on studies of bone marrow transplant donors

Cited by 18 publications

References 16 publications

Haematopoietic abnormalities after autologous stem cell transplantation in lymphoma patients

Haematopoietic abnormalities after autologous stem cell transplantation in lymphoma patients

Culturing human umbilical cord blood: a comparison of mononuclear vs CD34+ selected cells

Donor-derived 47, XXY in an unrelated cord blood transplant recipient

Contact Info

Product

Resources

About