Normal human endometrium in cell culture

Abstract

Separation of human endometrium into its epithelial and stromal components has been achieved through collagenase digestion and has permitted a study of these two cell populations under specific experimental culture conditions. The stromal cell populations showed a progesterone response, were easily handled in culture, and displayed a limited in vitro life span typical of human diploid fibroblasts. In contrast, epithelium only survived in short-term primary culture and showed no clear hormone response. High-density epithelial cultures remained viable for longer periods in culture. Comparisons between resurfacing endometrial epithelial cells in vivo and epithelial cells migrating from explants in vitro suggested that this initial epithelial migration in vitro was the counterpart of the repair response in vivo.

Interleukin-1β and the Endometrium: An Inhibitor of Stromal Cell Differentiation and Possible Autoregulator of Decidualization in Humans1

“…Cells were resuspended in a 0.8% ammonium chloride solution at 37°C for 5 min to lyse the red blood cells. After the cells were washed, the stromal cells were separated from the epithelial cells by using modifications of selective attachment [11] and selective trypsinization methods [12]. Briefly, the cells were suspended in DMEM containing 2% fetal bovine serum (FBS) (Hyclone, Logan, UT) and an antibiotic-antimycotic mixture (final concentrations: 25 U/ml penicillin, 25 pzg/ml streptomycin, and 2.5 g/ml amphotericin B).…”

Modeling the early endometriotic lesion: mesothelium-endometrial cell co-culture increases endometrial invasion and alters mesothelial and endometrial gene transcription

“…Endometrial stromal cells were cultured as described elsewhere (6)(7)(8). Briefly, the endometrium was minced and then enzymatically digested with 0.1% collagenase type I and 0.05% DNAse (Worthington Biochemical, Lakewood, NJ).…”

Imatinib decreases endometrial stromal cell transmesothial migration and proliferation in the extracellular matrix of modeled peritoneum

“…Previous studies had demonstrated similar rates of endometrial cell binding to commercially available LP9 PMCs when compared with PMCs derived from parietal peritoneum and ovarian surface epithelium (23).…”

“…Monolayer culture of ESCs were established as previously described elsewhere (21)(22)(23). Briefly, endometrium was mechanically dispersed with a scalpel then enzymatically digested with 0.1% collagenase type 1 and 0.05% DNAse.…”