Normal-incidence type solution immersed silicon (SIS) biosensor for ultra-sensitive, label-free detection of cardiac troponin I

Kim, Dong Hyung; Chegal, Won; Cho, Yong Jai; O, Sang Won; Le, Long V.; Diware, Mangesh S.; Paek, Se‐Hwan; Kim, Young Dong; Cho, Hyun Mo

doi:10.1016/j.bios.2020.112525

Cited by 5 publications

(5 citation statements)

References 47 publications

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“…Specifically, the SIS sensor operates by directing in through a buffer solution, a bio-layer, and a substrate layer (SiO2). In this con the buffer solution serves as the incident medium, whose role is to provide temperature and humidity environment; errors due to refractive index chan tremely eliminated at the Brewster angle, resulting in a sensitivity more tha higher than conventional ellipsometric sensors [21,22]. Using the SIS sensor analyzed the interaction between E. amylovora and the LptE-functionalized S Figure 6 shows the dose-response curve plotted against the concentrations of e by a logarithmic scale, which displays a linearity (R 2 > 0.992) for wide dynami tween 10 0 and 10 7 cells/mL.…”

Section: Single-cell Detection Of E Amylovoramentioning

confidence: 99%

“…A comparison of various diagnostic methods for E. amylovora detection is described in Table 1. A promising sensor platform based on silicon, called SIS sensor, has been developed that can replace the traditional gold film used in Surface Plasmon Resonance (SPR) sensors [20,21]. The traditional SPR sensors need an additional reference channel to eliminate the noise signal from refractive index changes of the buffer solution and the gold thin film induced by variations of surrounding environments such as temperature and flow conditions [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor

Lee,

Oh,

Kwon

et al. 2023

Sensors

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Herein, we developed a bio-functionalized solution-immersed silicon (SIS) sensor at the single-cell level to identify Erwinia amylovora (E. amylovora), a highly infectious bacterial pathogen responsible for fire blight, which is notorious for its rapid spread and destructive impact on apple and pear orchards. This method allows for ultra-sensitive measurements without pre-amplification or labeling compared to conventional methods. To detect a single cell of E. amylovora, we used Lipopolysaccharide Transporter E (LptE), which is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane of E. amylovora, as a capture agent. We confirmed that LptE interacts with E. amylovora via LPS through in-house ELISA analysis, then used it to construct the sensor chip by immobilizing the capture molecule on the sensor surface modified with 3′-Aminopropyl triethoxysilane (APTES) and glutaraldehyde (GA). The LptE-based SIS sensor exhibited the sensitive and specific detection of the target bacterial cell in real time. The dose–response curve shows a linearity (R2 > 0.992) with wide dynamic ranges from 1 to 107 cells/mL for the target bacterial pathogen. The sensor showed the value change (dΨ) of approximately 0.008° for growing overlayer thickness induced from a single-cell E. amylovora, while no change in the control bacterial cell (Bacillus subtilis) was observed, or negligible change, if any. Furthermore, the bacterial sensor demonstrated a potential for the continuous detection of E. amylovora through simple surface regeneration, enabling its reusability. Taken together, our system has the potential to be applied in fields where early symptoms are not observed and where single-cell or ultra-sensitive detection is required, such as plant bacterial pathogen detection, foodborne pathogen monitoring and analysis, and pathogenic microbial diagnosis.

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Section: Single-cell Detection Of E Amylovoramentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor

Lee,

Oh,

Kwon

et al. 2023

Sensors

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“…Early diagnosis of acute myocardial infarction (AMI), via high-sensitive detection of its specific biomarker, can effectively treat the disease and significantly reduce the mortality rate [1,2]. Troponin is a regulatory protein of the thin filament of striated muscle, released into the bloodstream 4 to 6 hours after AMI and the peak levels occur at 18 to 24 hours [3].…”

mentioning

confidence: 99%

“…Zhihe Guo and Xuyang Zhao have contributed equally to this work. prognostic information supporting risk stratification and highly accurate diagnostics at the early stage [2,9].…”

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confidence: 99%

“…Various highly sensitive biosensors and biosensing techniques have been continually proposed and evolving to enhance analytical performance for target biomarkers [2,10,11]. Foreign labeling or tracer agents, such as isotope, enzymes, metal nanoparticles and fluorescent materials, are developed to generate or amplify the weak sensing signal [2]. However, it requires a labeling process as a preparation step that is usually low yield, combining synthesis and purification [12].…”

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confidence: 99%

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Label‐free detection of cardiac troponin‐I with packaged thin‐walled microbubble resonators

Guo

Zhao

Zhou

et al. 2022

Journal of Biophotonics

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The measurement of cardiac troponin‐I (cTnI) is widely used for diagnosing acute myocardial infarction (AMI) diseases because of its myocardial specificity. Packaged microbubble resonators with thin wall are utilized for label‐free and specific detection of cTnI based on whispering gallery mode (WGM). This packaged structure can provide a good protection for the biosensor, improve the anti‐interference ability of the sensor and reduce the system noise. The theoretical detection limit of the biosensors for cTnI in phosphate buffer saline (PBS) is 0.4 ag mL−1 (0.02 aM). Furthermore, we demonstrated that the biosensors can be used to detect cTnI molecules in simulated serum and the theoretical detection limit is also 0.4 ag mL−1 (0.02 aM). These results are much far below the clinical cut‐off value and show a huge application potential for the detection of cardiac biomarkers of AMI.

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Subnanometer dextran analysis for biosensors using a tilt stage in atomic force microscopy

Kim

et al. 2022

Progress in Organic Coatings

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Normal-incidence type solution immersed silicon (SIS) biosensor for ultra-sensitive, label-free detection of cardiac troponin I

Cited by 5 publications

References 47 publications

Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor

Single-Cell Detection of Erwinia amylovora Using Bio-Functionalized SIS Sensor

Label‐free detection of cardiac troponin‐I with packaged thin‐walled microbubble resonators

Subnanometer dextran analysis for biosensors using a tilt stage in atomic force microscopy

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