Normal Inflammatory Bowel Disease Mucosa Conceals Alterations in Natural Killer Cell Activity

Abstract

3van To1 EAF, Verspaget HW, Peria AS, Lamers CBHW. Normal inflammatory bowel disease mucosa conceals alterations in natural killer cell activity. Scand J Gastroenterol 1992;27:999-1005.fion-major histocompatibility complex-restricted cytotoxicity or natural killer (NK) activity could be detected in all intestinal lamina propria mononuclear cell preparations of histologically normal mucosa from 57 patients with gastrointestinal disease. Similar levels of NK activity were detected among the different disease groups. Within the inflammatory bowel disease patient group, however, Crohn's disease patients showed a threefold higher level of NK activity than detected in ulcerative colitis patients. Cytotoxicity levels in Crohn's disease patients were also higher than in the control carcinoma patients, whereas ulcerative colitis patients had considerably lower cytotoxicity levels than the carcinoma patients. Thus, unaffected normal inflammatory bowel disease mucosa conceals alterations in NK activity which might occur before the inflammation. The colon adenocarcinoma cell line Caco-2 was found to be a representative target for detecting individual differences in NK activity of lamina propria mononuclear cells compared with standard K-562 targets. The latter can he of relevance when studying mucosal immunoregulatory mechanisms in intestinal disease. Scand J Gastroenterol Downloaded from informahealthcare.com by University of Sydney on 01/05/15 For personal use only. DISCUSSION Non-MHC-restricted cytotoxicity or NK activity in LPMC preparations from intestinal mucosa could be demonstrated Scand J Gastroenterol Downloaded from informahealthcare.com by University of Sydney on 01/05/15For personal use only.

Peripheral and Intestinal CD4+ T Cells With a Regulatory Phenotype in Pediatric Patients With Inflammatory Bowel Disease

“…Previous observations did not report alterations in the relative frequency of CD4+ T cells in either active or inactive adult IBD mucosal lesions (41), leaving open the possibility that our results reflect a specificity of the pediatric condition. The augmented presence of effector cytotoxic cells expressing CD56 receptor has been previously noted in active and inactive mucosae of adult patients with IBD (42,43) and could play a role in the mechanisms underlying the mucosal damage. In fact, CD56 receptor is expressed in the majority of NK cells, which represent an important component of the innate arm of the immune system, and also play an immunoregulatory role at mucosal sites, by participating in the instruction of the adaptive response (44–46); in addition, CD56 expression marks a T‐lymphocyte subset that bears effector‐like properties in vivo (44,47) and that is constitutively present in the lamina propria (48).…”

Integrated 16S rRNA sequencing and metagenomics insights into microbial dysbiosis and distinct virulence factors in inflammatory bowel disease

“…Functional analysis suggests different pathogenic mechanisms. A previous study also revealed that CD patients showed a 3-fold higher level of NK activity than that detected in UC patients (Van Tol et al, 1992 ). Additionally, the enrichment of reactive oxygen species in CD patients mediates the activation of NK cells (Sun et al, 2020 ).…”