Normal Pubertal Development: Part I: The Endocrine Basis of Puberty

Bordini, Brian; Rosenfield, Robert L.

doi:10.1542/pir.32-6-223

Cited by 82 publications

(76 citation statements)

References 20 publications

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“…In consultation with our endocrinologists, we determined that both assays are analytically and clinically equivalent, and the existing reference intervals established by the classic RIA method developed in our institution and published by our endocrinologists in the literature (34)(35)(36)(37)(38) are transferrable to the LC-MS/MS assay. The bias of this assay at the LOQ level was also not assessed, since there are currently no low E 2 reference materials available, and bias study by spiking standards and serially diluting up to 200-fold could result in large variation at the low end.…”

Section: Limitationsmentioning

confidence: 99%

High-Sensitivity Micro LC-MS/MS Assay for Serum Estradiol without Derivatization

Leung

Bridgman

et al. 2016

The Journal of Applied Laboratory Medicine

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Background: There are considerable demands to accurately measure estradiol (E 2 ) at low concentrations (<20 pg/mL) in postmenopausal women, men, pediatric patients, and patients receiving breast cancer treatment. Most current high-sensitivity LC-MS/MS E 2 methods require large sample volumes and involve complex sample preparations with dansyl chloride derivatization. Our study aims to develop a high-sensitivity, underivatized method using micro LC-MS/MS to reliably measure E 2 concentrations below 5 pg/mL by the use of low sample volume. Methods: A total of 290 μL of sample was mixed with internal standard (IS), E 2 -d4, and extracted with a mixture of hexane/ethyl acetate (90/10) (v/v). After extraction, sample was separated by Eksigent Ekspert™ micro LC 200 system with a flow rate of 35 μL/min in a total run time of 3.5 min and detected by SCIEX QTRAP 6500 mass spectrometer in a negative mode using transitions: 271/145 (quantifier) and 271/143 (qualifier). In this method, it was crucial to use HPLC columns with stability at a pH >10. Results:The validation study demonstrated broad linear ranges (3.0 -820.0 pg/mL) with r 2 > 0.999. Total precision was below 15% at all QC levels, and limit of quantification (LOQ) was 3.0 pg/mL. Our method showed good correlation with E 2 RIA (r 2 = 0.96, bias = −1.0 pg/mL) and modest correlation with E 2 Roche Cobas automated immunoassay (r 2 = 0.86, bias = 6.0 pg/mL). Conclusions:In conclusion, we developed and validated a routinely applicable micro LC-MS/MS method without derivatization for E 2 in blood samples with an LOQ of 3.0 pg/mL. IMPACT STATEMENTPatients under assessment of puberty delays, pubertal growth, gynecomastia, and efficiency of aromatase inhibitor (AI) treatment will benefit from the information presented here. Evidence presented on high-sensitivity LC-MS/MS assay for E 2 will allow better characterization of E 2 concentration in blood in the low measurement range. Knowledge in the field of LC-MS/MS method development for E 2 will be advanced by the information presented.

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Section: Limitationsmentioning

confidence: 99%

High-Sensitivity Micro LC-MS/MS Assay for Serum Estradiol without Derivatization

Leung

Bridgman

et al. 2016

The Journal of Applied Laboratory Medicine

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“…Diese Entwicklung ist vermutlich nicht allein auf die gegenwärtige Adipositasepidemie zurückzuführen. Auch verschiedene Umgebungsfaktoren, insbesondere endokrine Disruptoren, werden für diesen Trend verantwortlich gemacht [3]. In verschiedenen epidemiologischen Studien wurde zudem ein möglicher Zusammenhang zu einer Exposition mit Blei (verspäteter Pubertätsbeginn) und polybromierten Biphenylen (verfrühter Pubertätsbeginn) hergestellt [34].…”

Section: Pubertätsbeginn -Verlauf Und -Dauerunclassified

“…Die zunehmende Frequenz und Amplitude der Gonadotropinsekretion bewirken die vermehrte gonadale Synthese von Sexualsteroiden: Das luteinisierende Hormon (LH) regt in den interstitiellen Zellen von Ovar (Thekazellen) und Hoden (Leydig-Zellen) die Synthese von androgenen Präkursoren und Testosteron (Hoden) an, während das follikelstimulierende Hormon (FSH) über die Stimulierung von Aromatasen die Synthese von Östrogen aus androgenen Vorstufen bewirkt [3]. FSH führt darüber hinaus über die Anregung der Granulosa-(Ovar) und Sertoli-Zellen (Hoden) zur Ausreifung von Eizellen und Spermien.…”

Section: Pubertätsbeginn -Verlauf Und -Dauerunclassified

Vorzeitiger, verspäteter und ausbleibender Pubertätsbeginn

Land

2012

Monatsschr Kinderheilkd

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“…LH acts on ovarian interstitial (theca) cells to promote synthesis of estradiol's androgenic precursors and on testicular Leydig cells to induce testosterone secretion. FSH acts on ovarian granulosa cells and testicular Sertoli cells to promote gametogenesis (9).…”

Section: Introductionmentioning

confidence: 99%

“…These include thelarche, the onset of breast development; pubarche, the appearance of pubic hair; gonadarche, the onset of sex hormone production by the gonads; menarche, the initiation of menses; and spermarche, the appearance of spermatozoa in semen (9). Pubertal changes in pubic hair, characteristics of external genitalia in males, and breast development in females are conventionally described in terms of five progressive physical stages as defined by Tanner (86).…”

Section: Introductionmentioning

confidence: 99%

Nutritional Determinants of the Timing of Puberty

Villamor

Jansen

2016

Annu. Rev. Public Health

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The timing of puberty has important public health, clinical, and social implications. The plasticity of sexual development onset could be a mechanism that adapts to prevailing environmental conditions. Early-life nutrition may provide cues for the environment's suitability for reproduction. This review focuses on recent developments in our understanding of the role of diet in the timing of sexual maturation. Population-based observational studies consistently indicate that childhood obesity is related to the earlier onset of puberty in girls. Similarly, intake of animal foods has been associated with earlier sexual development, whereas vegetable protein intake is related to delayed maturation. Evidence for prenatal nutrition, infant feeding practices, and childhood intake of fat, carbohydrate, and micronutrients is inconsistent. Secondary analyses of prenatal and early-life randomized nutritional interventions with extended follow-up through peripubertal years would help clarify the role of nutrition in the timing of sexual maturation.

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Normal Pubertal Development: Part I: The Endocrine Basis of Puberty

Abstract: Objectives After completing this article, readers should be able to: 1. Explain how puberty is regulated by the hypothalamic-pituitary-gonadal axis. 2. Describe the hormonal interactions involved in pubertal development in boys and girls.

Cited by 82 publications

References 20 publications

High-Sensitivity Micro LC-MS/MS Assay for Serum Estradiol without Derivatization

High-Sensitivity Micro LC-MS/MS Assay for Serum Estradiol without Derivatization

Vorzeitiger, verspäteter und ausbleibender Pubertätsbeginn

Nutritional Determinants of the Timing of Puberty

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