Normal variation of bone marrow B‐cell precursors according to age – reference ranges for studies in myelodysplastic syndromes in Brazil

Lorand-Metze, Irene; Longhini, Ana Leda; Oliveira-Duarte, Gislaine; Correia, Rodolfo Patussi; Santos-Silva, Maria Cláudia; Yamamoto, Masahiro; Sandes, Alex Freire; Oliveira, Anita Frisanco; Souto, Elizabeth Xisto; Ikoma, Maura R. V.; Pereira-Cunha, F.G.; Beltrame, Míriam Perlingeiro; Metze, Konradin

doi:10.1002/cyto.b.21604

Cited by 11 publications

(19 citation statements)

References 31 publications

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“…Concerning B-cell progenitors (hematogones type I), using the same strategy we found that patients with hematogones within the upper quartile had a better survival. This value corresponded to the normal range for age in our population, as previously demonstrated in a Brazilian multicenter study 20 . Similar results had been described by other studies concerning MDS 28 , 29 and ALL in remission after induction 30 .…”

Section: Discussionsupporting

confidence: 90%

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A simple score derived from bone marrow immunophenotyping is important for prognostic evaluation in myelodysplastic syndromes

Vido-Marques

Reis-Alves

Saad

et al. 2020

Sci Rep

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Immunophenotyping of bone marrow (BM) precursors has been used as an ancillary diagnostic tool in myelodysplastic syndromes (MDS), but there is no general agreement about which variables are the most relevant for prognosis. We developed a parsimonious prognostic model based on BM cell populations well-defined by phenotype. We analyzed 95 consecutive patients with primary MDS diagnosed at our Institution between 2005 and 2012 where BM immunophenotyping had been performed at diagnosis. Median follow-up: 42 months (4–199). Median age: 67 years (33–79). According to IPSS-R, 71 cases were low or intermediate risk. Flow variables significant in the univariate Cox analysis: “%monocytes/TNCs”, “% CD16+ monocytes/TNCs”, “total alterations in monocytes”, “% myeloid CD34+ cells”, “number of abnormal expressions in myeloblasts” and “% of B-cell progenitors”. In the multivariate model remained independent: “% myeloid CD34+ cells”, B-cell progenitors” and “% CD16+ monocytes/TNCs”. These variables were categorized by the extreme quartile risk ratio strategy in order to build the score: % myeloid CD34+ cells” (≥ 2.0% = 1 point), B-cell progenitors” (< 0.05% 1 point) and “CD16+ monocytes/TNCs” (≥ 1.0% 1 point). This score could separate patients with a different survival. There was a weak correlation between the score and IPSS-R. Both had independent prognostic values and so, the flow score adds value for the prognostic evaluation in MDS.

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Section: Discussionsupporting

confidence: 90%

“…Values for myeloid CD34 + cells were considered normal when below 2% 6 . For hematogones (H1), the normal Brazilian reference values for age were used 20 .…”

Section: Methods and Development Of The Scorementioning

confidence: 99%

A simple score derived from bone marrow immunophenotyping is important for prognostic evaluation in myelodysplastic syndromes

Vido-Marques

Reis-Alves

Saad

et al. 2020

Sci Rep

Self Cite

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“…However, after the completion of the induction chemotherapy, the hematopoietic recovery ability was better in patients with higher levels of hematogones. In fact, earlier reports have also demonstrated that hematogones were rarely detected in patients with myelodysplastic syndrome or aplastic anemia 28–31 . Therefore, our data consistent with previous work which suggest that the emergence of hematogones may be closely related to the active hematopoietic status.…”

Section: Discussionmentioning

confidence: 94%

The prognostic significance of hematogones and CD34+ myeloblasts in bone marrow for adult B-cell lymphoblastic leukemia without minimal residual disease

Liao

Zheng

Jin

et al. 2019

Sci Rep

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This study was aimed to dissect the prognostic significances of hematogones and CD34+ myeloblasts in bone marrow for adult B-cell acute lymphoblastic leukemia(ALL) without minimal residual disease(MRD) after the induction chemotherapy cycle. A total of 113 ALL patients who have received standardized chemotherapy cycle were analyzed. Cases that were not remission after induction chemotherapy or have received stem cell transplantation were excluded. Flow cytometry was used to quantify the levels of hematogones and CD34+ myeloblasts in bone marrow aspirations, and the patients were grouped according to the levels of these two precursor cell types. The long-term relapse-free survival(RFS) and recovery of peripheral blood cells of each group after induction chemotherapy were compared. The results indicated that, after induction chemotherapy, patients with hematogones ≥0.1% have a significantly longer remission period than patients with hematogones <0.1% (p = 0.001). Meanwhile, the level of hematogones was positively associated with the recovery of both hemoglobin and platelet in peripheral blood, while CD34+ myeloblasts level is irrelevant to the recovery of Hb and PLT in peripheral blood, level of hematogones and long-term prognosis. This study confirmed hematogones level after induction chemotherapy can be used as a prognostic factor for ALL without MRD. It is more applicable for evaluation prognosis than CD34+ myeloblasts.

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“…Myelodysplastic syndromes (MDS) are deemed a stem-cell disorders characterized by dysplasia and bone marrow failure that may evolve into AML, and our journal has enjoyed recent contributions on this topic (23,24). Finally, to round out the theme of aberrant myelopoiesis in this issue is the contribution from Gardikas et al (25), related to MDS.…”

Section: Issue Highlightsmentioning

confidence: 99%

From the Editor: Thank you! Remembrances and Issue Highlights

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2019

Cytometry Part B Clinical

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Normal variation of bone marrow B‐cell precursors according to age – reference ranges for studies in myelodysplastic syndromes in Brazil

Cited by 11 publications

References 31 publications

A simple score derived from bone marrow immunophenotyping is important for prognostic evaluation in myelodysplastic syndromes

A simple score derived from bone marrow immunophenotyping is important for prognostic evaluation in myelodysplastic syndromes

The prognostic significance of hematogones and CD34+ myeloblasts in bone marrow for adult B-cell lymphoblastic leukemia without minimal residual disease

From the Editor: Thank you! Remembrances and Issue Highlights

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