Normal Variations with Aging of the Amount of Hematopoietic Tissue in Bone Marrow from the Anterior Iliac Crest: A Study Made from 177 Cases of Sudden Death Examined by Necropsy

Hartsock, Robert J.; Smith, Edward B.; Petty, Charles S.

doi:10.1093/ajcp/43.4.326

Cited by 274 publications

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“…BMB cellularity compared to an age-matched control was assessed by visual examination, according to Harstock et al [18], and graded into three groups as follows: normocellular (30 to 50% of intertrabecular space occupied by hematopoietic tissue), hypercellular (>50% of intertrabecular space occupied by hematopoietic tissue), and hypocellular (<30% of intertrabecular space occupied by hematopoietic tissue). The amount of erythroid, myeloid, and megakaryocytic precursors was assessed as decreased, normal, or increased by simultaneously taking into account BM cellularity and the myeloid-erythroid ratio [19].…”

Section: Histologic Parameters and Methods Of Assessmentmentioning

confidence: 99%

Bone marrow histological findings in systemic lupus erythematosus with hematologic abnormalities: A clinicopathological study

et al. 2006

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Background: The histopathologic features characterizing the involvement of the bone marrow (BM) in systemic lupus erythematosus (SLE) have not been systematically analyzed to date.Objectives: The aim of this study was to assess morphologic and immunohistochemical characteristics of BM involvement in SLE.Patients and methods: Clinical and serological data of 40 SLE patients with unexplained cytopenias were studied. Ten patients with myelodysplasia of refractory anemia (RA) were used as controls. BM aspiration, BM biopsy (BMB), and immunohistochemistry were carried out in patients and controls. BM fibrosis, BM necrosis, stromal edema, and abnormal localization of immature precursors (ALIP) were assessed according to standard criteria.Results: Dyserythropoiesis and megakaryocytic atypias were uniform findings in SLE patients. The disruption of the normal BM architecture was a predominant SLE BM feature affecting cells of all three hemopoietic lineages, with both erythroid and megakaryocytic precursors tending to assume paratrabecular locations and ALIP aggregates being present in 27 cases. In addition, BM was hypocellular in 23 cases. BM necrotic alterations were evident in 90% of the cases. The density of reticulin content was generally increased. Vascular changes including dilatation of sinuses were manifest and were associated with the presence of necrotic alterations (P 5 0.008). Hemoglobin levels correlated inversely with the presence of ALIP (P 5 0.016). Upon comparing BMB features between SLE and RA controls there were striking similarities.Conclusions: BMB in patients with SLE and unexplained cytopenias presents a variety of histopathologic findings including BM necrosis, stromal alterations, hypocellularity, dyspoiesis, and distortion of normal BM architecture, characterized primarily by the presence of ALIP aggregates. Am. J. Hematol. 81:590-597, 2006. V V C 2006 Wiley-Liss, Inc.

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Section: Histologic Parameters and Methods Of Assessmentmentioning

confidence: 99%

Bone marrow histological findings in systemic lupus erythematosus with hematologic abnormalities: A clinicopathological study

et al. 2006

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“…The transition occurs over two decades in a predictable sequence, beginning in the periphery of the skeleton and extending in a symmetric, centripetal manner into the central skeleton (Fig. 2a) [1,7]. Postnatally, marrow conversion starts in the terminal phalanges.…”

Section: Conversion Of Bone Marrowmentioning

confidence: 99%

“…Despite its unclear physiological role, fat is the major component of both yellow marrow and (to a lesser extent) red marrow. In the iliac crests of adults, approximately 50-60% of marrow is hematopoietic [7]. The cellular composition of red marrow consists of 60% hematopoietic cells and 40% adipocytes; its chemical composition is 40-60% lipid, 30-40% water, and 10-20% proteins [1,2].…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance imaging of bone marrow in oncology, Part 1

Hwang

Panicek

2007

Skeletal Radiol

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Magnetic resonance imaging plays an integral role in the detection and characterization of marrow lesions, planning for biopsy or surgery, and post-treatment followup. To evaluate findings in bone marrow on MR imaging, it is essential to understand the normal composition and distribution of bone marrow and the changes in marrow that occur with age, as well as the basis for the MR signals from marrow and the factors that affect those signals. The normal distribution of red and yellow marrow in the skeleton changes with age in a predictable sequence. Important factors that affect MR signals and allow detection of marrow lesions include alterations in fat-water distribution, destruction of bony trabeculae, and contrast enhancement. This two-part article reviews and illustrates these issues, with an emphasis on the practical application of MR imaging to facilitate differentiation of normal marrow, tumor, and treatment-related marrow changes in oncology patients.

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“…5 Four hundred cell differential counts were performed on Giemsa-stained sections. The myeloid:erythroid ratio (M:E) was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…1,[3][4][5][6][7][8] Although in one report reduced activity of the bone marrow was considered to be responsible for the cytopenias, 9 subsequent studies have failed to confirm this and instead, increased marrow activity has been demonstrated. Several mechanisms have been suggested to account for pancytopenia in VL, the most acceptable of which is histiocytic hemophagocytosis.…”

Section: Figure 6 Marrow With Mild Diffuse Increased Fibrosis (250x)mentioning

confidence: 99%

Bone Marrow Histopathological Changes in Visceral Leishmaniasis

Al-Sohaibani

1996

Ann Saudi Med

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Over a period of two years (June 1989-May 1991, bone marrow trephine biopsies from 16 patients with visceral leishmaniasis were examined histologically to assess the correlation between the peripheral blood counts and bone marrow status. Only 25% of patients had normal peripheral blood counts. Of the remainder, 25% had anemia alone, 25% had anemia with thrombocytopenia, and 25% had pancytopenia. The anemia was microcytic/hypochromic in 88.7% of cases and normocytic in 12.3% of cases. Thrombocytopenia was a notable feature, with mean platelet count of 115 ± 72.47 x 10 9 /L, and eight patients having thrombocytopenia below 120 x 10 9 /L. There was diffuse bone marrow hypercellularity in 13 cases and focal hypocellularity in three. The increased cellularity was attributable to trilineage hyperplasia, with a predominance of erythroid activity in most cases. Increase in the histiocytic population was a prominent feature in all the cases. In 10 cases, most of the histiocytes were found to be full of LD bodies, while in the other six, the parasite load was much less. Megakaryocytes were abundant in number, forming aggregates in most cases. Other notable features included erythrophagocytosis (nine cases) and moderate perivascular plasmacytosis (13 cases). Ann Saudi Med 1996;16(3):304-307. Leishmaniasis. 1996; 16(3): 304-307 Visceral leishmaniasis (VL), also known as kala-azar, is endemic in the southern (Gizan Province) and southwestern regions (Asir Province) of Saudi Arabia. MO Al-Sohaibani, Bone Marrow Histopathological Changes in Visceral1-3 On the other hand, the cutaneous form of leishmaniasis is prevalent in the eastern and central regions, particularly in Al-Hassa and Al-Qassim.2 Previous reports from the Kingdom 1-4 have dealt with the clinical features of VL, which are found to be similar to those described in patients from elsewhere.To the best of our knowledge, a detailed study demonstrating bone marrow findings in patients with VL has no routine histologic techniques. Four micron sections were cut and stained with hematoxylin and eosin, Giemsa, and reticulin stain by silver impregnation. Material and MethodsThe study was conducted on 18 patients of pediatric age group, admitted to King Fahad Hospital at Al-Baha with clinical features suggestive of VL. The duration of their illness ranged from two weeks to one year. Each patient underwent a complete clinical workup, including a detailed history and physical examination, followed by diagnostic bone marrow or splenic puncture in which Leishman-Donovan bodies (LDB) or amastigotes were demonstrated.Adequate trephine bone marrow biopsies were obtained from the posterior iliac crest with a Jamshidi needle in 16 patients, who are the subjects of this report. Biopsies were fixed in 10% neutral buffered formalin, then decalcified for one hour and processed by routine histologic techniques. Four micron sections were cut and stained with hematoxylin and eosin, Giemsa and reticulin stain by silver impregnation.Microscopically, the marrow cellularity was assessed acco...

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Normal Variations with Aging of the Amount of Hematopoietic Tissue in Bone Marrow from the Anterior Iliac Crest: A Study Made from 177 Cases of Sudden Death Examined by Necropsy

Cited by 274 publications

References 0 publications

Bone marrow histological findings in systemic lupus erythematosus with hematologic abnormalities: A clinicopathological study

Bone marrow histological findings in systemic lupus erythematosus with hematologic abnormalities: A clinicopathological study

Magnetic resonance imaging of bone marrow in oncology, Part 1

Bone Marrow Histopathological Changes in Visceral Leishmaniasis

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