Normalizing sweet orange (C. sinensis (L.) osbeck) somatic embryogenesis with semi-permeable membranes

Niedz, Randall P.; Hyndman, Scott E.; Wynn, Eldridge T.; Bausher, Michael G.

doi:10.1079/ivp2002331

Cited by 36 publications

(23 citation statements)

References 23 publications

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“…The G1 callus line had been in culture for several years and had lost its capacity for direct protoplast regeneration. Embryos were recovered from all four fusion combinations and transferred to 0.22 mm acetate plus membrane filters placed directly on fresh EME solid medium containing 0.15 M maltose instead of sucrose (Perez et al 1998) to expedite growth and development (Niedz et al 2002). Great differences in embryo and plantlet recovery were found from the four fusion combinations.…”

Section: Resultsmentioning

confidence: 99%

“…The procedures for protoplast isolation, PEG-mediated fusion, and culture were according to Grosser and Gmitter (1990). Regenerated embryos were also cultured over 0.22 mm acetate plus membrane filters, which were put on fresh EME solid medium (Grosser and Gmitter 1990) containing 0.15 M maltose instead of sucrose to normalize and enlarge the embryos (Niedz et al 2002). The regenerated shoots were grafted in vitro or onto greenhouse rootstock seedlings to expedite growth.…”

Section: Protoplast Fusion and Plant Regenerationmentioning

confidence: 99%

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Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness

et al. 2004

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CMS (cytoplasmic male sterility) can be controlled by the mitochondrion genome in higher plants, including Satsuma mandarin. Somatic fusion experiments in citrus combining embryogenic callus protoplasts of one parent with leaf protoplasts of a second parent often produce cybrid plants of the leaf parent, a phenomenon occurring most often with interspecific fusion combinations. In an attempt to practically exploit this cybridization phenomenon, we conducted somatic fusion experiments combining embryogenic suspension-derived protoplasts of Satsuma mandarin, Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a male-sterile cultivar, with leaf protoplasts of other seedy types--Hirado Buntan Pink pummelo (HBP) [Citrus grandis (L.) Osbeck], Sunburst mandarin (C. reticulata Blanco), Orie Lee hybrid (C. reticulata cv. Clementine x Murcott tangor), and Murcott tangor [C. reticulata x C. sinensis (L.) Osbeck], respectively--in an attempt to generate seedless cybrids by the targeted transfer of CMS. The genetic identities of regenerated plants from all four parental combinations were determined by flow cytometry, SSR, CAPS (or PCR-RFLP), RFLP, and chloroplast-SSR analyses. Regenerated plants from the first three parental combinations were diploids, and the cybrid nature of G1 + HBP with the mitochondrion genome from G1 and the chloroplast genome from HBP was confirmed, whereas the cybrid nature of the remaining two combinations was difficult to confirm because of the close phylogenetic relatedness of both fusion parents, as expected. Plants from G1 + Murcott were confirmed as tetraploid somatic hybrids. This is the first report of targeted citrus cybrid production by symmetric fusion with male-sterile Satsuma as the callus parent and other seedy cultivars as the leaf parents.

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Section: Resultsmentioning

confidence: 99%

Section: Protoplast Fusion and Plant Regenerationmentioning

confidence: 99%

Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness

et al. 2004

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“…Although in the genus Citrus somatic embryogenesis was reported a long time ago (Maheshwari and Rang Swamy 1958), a number of difficulties have been encountered in establishing reliable protocols. In the abundant literature on Citrus somatic embryogenesis, it appears that results vary greatly depending on the genotypes (Mendes-da-Gloria et al 2001, Tomaz et al 2001, Niedz et al 2002, Ramirez et al 2003. The embryogenic potential of citrus varied with genotype and type of explant.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration and somatic embryogenesis in citrus

Mohamed¹,

Gomaa²,

Danial

2015

Plant Tissue Cult. & Biotech.

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Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT-PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV-free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant-dependant.

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“…In the abundant literature on Citrus somatic embryogenesis, it appears that results vary greatly depending on the genotypes (Mendes-da- , Tomaz et al 2001, Niedz et al 2002, Ramirez et al 2003.…”

Section: Introductionmentioning

confidence: 99%

“…Beyond this stage, embryo development often shows varying degrees of abnormality (Button et al 1974, Niedz et al 2002. In Citrus sinensis, for example, somatic embryos displayed abnormal morphology (non-responsive or teratoma type embryo) and the effects of various semipermeable membranes were studied to normalize embryo development (Niedz et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Morphological and anatomical observations of abnormal somatic embryos from anther cultures of Citrus reticulata

Benelli̇

Germanà²,

Ganino³

et al. 2010

Biologia plant.

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A morphological and anatomical study of regenerants obtained from mandarin anther culture was carried out. Beside morphologically normal somatic embryos, abnormal structures were originated in the course of somatic embryogenesis. Anatomical anomalies can be found at several growth stages, causing the formation of slender stems, stubby structures, non-functional leaves. When too long, some structures are subject to shedding, with the formation of various abscission zones. Most of them are subject to degeneration, although many are capable of further, localized, morphogenesis. A thorough knowledge of morphology and anatomy of normal and abnormal regenerants could make possible to select and subculture the lines considered most suitable for conversion into plantlets.Additional key words: mandarin, in vitro culture, anatomy, abnormal structures.

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Normalizing sweet orange (C. sinensis (L.) osbeck) somatic embryogenesis with semi-permeable membranes

Cited by 36 publications

References 23 publications

Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness

Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness

In vitro regeneration and somatic embryogenesis in citrus

Morphological and anatomical observations of abnormal somatic embryos from anther cultures of Citrus reticulata

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