Norovirus contamination on French marketed oysters

Schaeffer, Julien; Saux, Jean-Claude Le; Lora, Monica; Atmar, Robert L.; Guyader, Françoise S. Le

doi:10.1016/j.ijfoodmicro.2013.07.022

Cited by 49 publications

(27 citation statements)

References 39 publications

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“…Amounts of 10 9 genome copies MNV-1 and 10 8 genome copies Mengovirus had the overall best suitability for the three matrices tested with regard to sensitivity and reproducibility. The same MNV-1 input was used in our previous studies Martin-Latil et al, 2012a) whereas the Mengovirus input was higher than that used in previous studies (10 5 and 10 6 genome copies) (Comelli et al, 2008;Schaeffer et al, 2013). The suitability of using these input amounts was confirmed because neither MNV-1 nor Mengovirus hampered the recovery of HAV and human norovirus in bottled water, lettuce or semi-dried tomatoes.…”

Section: Discussionmentioning

confidence: 63%

Determination of which virus to use as a process control when testing for the presence of hepatitis A virus and norovirus in food and water

Hennechart-Collette

Martin‐Latil

Guillier

et al. 2015

International Journal of Food Microbiology

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Section: Discussionmentioning

confidence: 63%

Determination of which virus to use as a process control when testing for the presence of hepatitis A virus and norovirus in food and water

Hennechart-Collette

Martin‐Latil

Guillier

et al. 2015

International Journal of Food Microbiology

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“…In contrast to GI and GII NoVs, GIV strains are rarely discovered in oyster samples but are usually identified in humans (GIV.1) (32, 33), dogs (GIV.1) (34-36), cats (GIV.2) (37), and lions (GIV.2) (38). The observation of relatively few GIV strains in oysters may be due to the fact that most routine screening and diagnostic studies on oysters so far have focused primarily on GI and GII NoVs (18,23,39,40). The mechanisms by which oysters accumulate this rare genogroup of NoVs remain to be elucidated.…”

Section: Gu726163) Was Isolated From Oyster Samples In Hong Kong In mentioning

confidence: 99%

“…Accordingly, it seems unlikely that a high number of NoVs is maintained in oysters via nonspecific binding mechanisms. Given that the diagnostic limitation of NoVs is normally 10 to 1,000 copies per g of oyster tissue (23,40,51), it is possible to detect NoVs only if there is a high abundance in the oyster samples tested. Taking these considerations together, the possibility of nonspecific attachment seems an unlikely cause of the diverse NoV genotypes observed in oysters.…”

Section: Gu726163) Was Isolated From Oyster Samples In Hong Kong In mentioning

confidence: 99%

Molecular Epidemiology of Oyster-Related Human Noroviruses and Their Global Genetic Diversity and Temporal-Geographical Distribution from 1983 to 2014

Cai

et al. 2015

Appl Environ Microbiol

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c Noroviruses (NoVs) are a leading cause of epidemic and sporadic cases of acute gastroenteritis worldwide. Oysters are well recognized as the main vectors of environmentally transmitted NoVs, and disease outbreaks linked to oyster consumption have been commonly observed. Here, to quantify the genetic diversity, temporal distribution, and circulation of oyster-related NoVs on a global scale, 1,077 oyster-related NoV sequences deposited from 1983 to 2014 were downloaded from both NCBI GenBank and the NoroNet outbreak database and were then screened for quality control. A total of 665 sequences with reliable information were obtained and were subsequently subjected to genotyping and phylogenetic analyses. The results indicated that the majority of oyster-related NoV sequences were obtained from coastal countries and regions and that the numbers of sequences in these regions were unevenly distributed. Moreover, >80% of human NoV genotypes were detected in oyster samples or oysterrelated outbreaks. A higher proportion of genogroup I (GI) (34%) was observed for oyster-related sequences than for nonoyster-related outbreaks, where GII strains dominated with an overwhelming majority of >90%, indicating that the prevalences of GI and GII are different in humans and oysters. In addition, a related convergence of the circulation trend was found between oyster-related NoV sequences and human pandemic outbreaks. This suggests that oysters not only act as a vector of NoV through environmental transmission but also serve as an important reservoir of human NoVs. These results highlight the importance of oysters in the persistence and transmission of human NoVs in the environment and have important implications for the surveillance of human NoVs in oyster samples. N orovirus (NoV) is known as the leading cause of nonbacterial acute gastroenteritis in humans and can infect people of all ages across the world (1). As a member of the family Caliciviridae, NoV is a nonenveloped, positive-sense, single-stranded RNA virus with a linear genome that contains three open reading frames (ORFs) (2). The genus Norovirus currently contains at least 6 distinct genogroups (genogroup I [GI], GII, GIII, GIV, GV, and GVI), each of which has been subdivided into multiple genotypes (3, 4). GI, GII, and GIV strains have been detected in humans and are further subdivided into 9, 22, and 2 genotypes, respectively (3, 4). One important genotype, genogroup II genotype 4 (GII.4), has been recognized recently as the predominant cause of major viral gastroenteritis epidemics worldwide (5-7) and contains a number of genetic variants (4,8).Over the past 20 years, new epidemic variants of GII.4 have emerged every 2 to 3 years (9) and usually have become the dominant strains in every season (7, 10, 11). For example, the Yerseke 2006a variant emerged in 2006, disappeared in 2008, and was replaced by the Den Haag 2006b variant (see Fig. S1 in the supplemental material). The New Orleans 2009 variant was the major player in the worldwide NoV outbreaks from 2010 to ...

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“…Thus, oysters can be used as bio-indicators for water contamination, and their consumption can result in risks to human health [6,30]. In addition, many viral and non-viral pathogens, including those of humans, can be isolated from oyster, such as norovirus [27], Cryptosporidium parvum [33], Vibrio cholera [14], and ostreid herpes virus 1 [7,24].…”

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confidence: 99%

Oysters as hot spots for mimivirus isolation

et al. 2014

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Viruses are ubiquitous organisms, but their role in the ecosystem and their prevalence are still poorly understood. Mimiviruses are extremely complex and large DNA viruses. Although metagenomic studies have suggested that members of the family Mimiviridae are abundant in oceans, there is a lack of information about the association of mimiviruses with marine organisms. In this work, we demonstrate by molecular and virological methods that oysters are excellent sources for mimiviruses isolation. Our data not only provide new information about the biology of these viruses but also raise questions regarding the role of oyster consumption as a putative source of mimivirus infection in humans.

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Norovirus contamination on French marketed oysters

Cited by 49 publications

References 39 publications

Determination of which virus to use as a process control when testing for the presence of hepatitis A virus and norovirus in food and water

Determination of which virus to use as a process control when testing for the presence of hepatitis A virus and norovirus in food and water

Molecular Epidemiology of Oyster-Related Human Noroviruses and Their Global Genetic Diversity and Temporal-Geographical Distribution from 1983 to 2014

Oysters as hot spots for mimivirus isolation

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