Noroviruses Distinguish between Type 1 and Type 2 Histo-Blood Group Antigens for Binding

Shirato, Haruko; Ogawa, Shinpei; Ito, Hiromi; Sato, Takashi; Kameyama, Akihiko; Narimatsu, Hisashi; Zheng, Xuejun; Miyamura, Tatsuo; Wakita, Takaji; Ishii, Koji; Takeda, Naokazu

doi:10.1128/jvi.00802-08

Cited by 150 publications

(172 citation statements)

References 47 publications

Supporting

Mentioning

159

Contrasting

Order By: Relevance

“…Chiba VLPs were used to prepare rabbit antisera (Kobayashi et al, 2000a) and to examine their interaction with histo-blood group antigens (Shirato et al, 2008). As reported in Kobayashi et al (2000a), when recombinant baculoviruses encoding the wild-type Chiba ORF2 gene infected Tn5 insect cells, particles smaller than naturally occurring norovirus particles (23 nm species) were generated, together with normal-size (38 nm species) particles (Fig.…”

mentioning

confidence: 99%

“…These results indicated that the 50 kDa proteins retained the region where rat antisera against the triplemutant VLPs reacted, and that the antigenicity was preserved despite the introduction of the triple mutation. Shirato et al (2008) have previously shown that the wildtype VLPs from the Chiba strain bound to synthetic type A carbohydrates of histo-blood group antigens as well as Lewis a and Lewis b carbohydrates. Here we examined whether or not the triple-mutant VLPs have the same characteristics as the wild-type VLPs.…”

mentioning

confidence: 99%

“…Here we examined whether or not the triple-mutant VLPs have the same characteristics as the wild-type VLPs. ELISA-based binding assays using biotinylated multivalent carbohydrate reagents (Glycotech) were performed as described previously (Shirato et al, 2008). Briefly, multivalent carbohydratebiotin reagents (Glycotech) were immobilized onto 96-well streptavidin-coated plates (Thermo Electron Corporation), and 100 ml of VLPs (1 mg ml…”

mentioning

confidence: 99%

See 2 more Smart Citations

Assembly of homogeneous norovirus-like particles accomplished by amino acid substitution

Someya

Shirato

Hasegawa

et al. 2011

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

Infection of insect cells with recombinant baculoviruses carrying the VP1 gene from Chiba strain norovirus resulted in the production of 57 and 50 kDa proteins, and the assembly of a smaller, 23 nm form of the virus-like particles (VLPs), together with the normal, 38 nm form of the VLPs. The N-terminal residues of the 57 and 50 kDa proteins were Ala4 and Thr45, respectively. When the tripeptide Leu43-Ala44-Thr45 was changed to Ala-Pro-Val, only 38 nm VLPs were assembled. The 38 nm VLPs showed essentially the same pattern of carbohydrate binding as the 23 nm VLPs, despite the significant difference in the degree of Lewis b antigen binding.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Assembly of homogeneous norovirus-like particles accomplished by amino acid substitution

Someya

Shirato

Hasegawa

et al. 2011

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Different NoVs have highly variable HBGA binding patterns and target different tissues, which may affect disease course [27]. Virus-like particles from the GII-4 genocluster demonstrated that variation in the receptor binding domain showed differential HBGA binding and altered antigenicity.…”

Section: Pathogenesis and Immunitymentioning

confidence: 99%

Noroviruses: Recent Updates

Chung

2012

Pediatr Gastroenterol Hepatol Nutr

View full text Add to dashboard Cite

Noroviruses (NoVs) are one major etiologic agent in acute gastroenteritis (AGE) in all ages and are the primary cause of food-borne gastroenteritis worldwide. GII-4 NoVs has predominated since 1990s, and novel recombinant strains have been reported worldwide. Researchers face difficulties in making vaccines and therapeutic agents against NoVs due to the lack of cell culture and animal-model systems and the rapid emergence of novel variant strains. Recently, a randomized clinical trial for intranasal NoVs vaccine has been reported, which casts a light in the way of vaccine production. This review discusses the recent findings on the structure, immunity, and vaccination of NoVs. (Pediatr Gastroenterol Hepatol Nutr 2012; 15: 1∼7)

show abstract

“…[15][16][17][18] As the understanding of these biological functions increases, the need for practical 55 synthetic procedures of oligosaccharides in large quantities has become a major subject. Organic chemical methods for obtaining them have been developed, 19-21 but they involve several elaborate protection and deprotection procedures.…”

Section: Introductionmentioning

confidence: 99%

Solvents derived from glycerol modify classical regioselectivity in the enzymatic synthesis of disaccharides with Biolacta β-galactosidase

et al. 2011

View full text Add to dashboard Cite

Green solvents made from glycerol change the classical regioselectivity of Biolacta N o 5 -galactosidase, from (1→4) toward (1→6) linkages when 2M concentration was used. In order to explain these results, the non-proteic compounds present in Biolacta preparation were separated by precipitation with 10 ammonium sulfate and the remaining protein extract was used to set reactions with appropriate organic solvents to find that the regioselectivity towards the β(1 6) isomer is retained. According to proteomic analysis, a 98% homology between Streptococcus pneumoniae and Biolacta -galactosidase preparation was found. With these data, molecular modelling was done and predicts a tridimensional interaction in the enzyme active site with the donor (GlcNAc) and the water-solvent mixture that explains this 15 phenomenon.

show abstract

Noroviruses Distinguish between Type 1 and Type 2 Histo-Blood Group Antigens for Binding

Cited by 150 publications

References 47 publications

Assembly of homogeneous norovirus-like particles accomplished by amino acid substitution

Assembly of homogeneous norovirus-like particles accomplished by amino acid substitution

Noroviruses: Recent Updates

Solvents derived from glycerol modify classical regioselectivity in the enzymatic synthesis of disaccharides with Biolacta β-galactosidase

Contact Info

Product

Resources

About