Abstract. The environmental, hydrological and climate dynamics were assessed in Northern Eurasia during the Holocene. The reconstructions are based on oxygen isotope composition of lacustrine diatom silica ( 18Odiatom) preserved in sediment cores from Ladoga, Bolshoye Shchuchye and Emanda lakes. Interpretation of the 18Odiatom data is supported by a comprehensive study of modern isotope hydrology and analysis of local and regional proxies. The Northern Eurasia 18Odiatom records are characterized by pronounced short term variations (1.5 5 ), pointing to the unstable climatic and hydrological conditions in the study regions. All records have clearly demonstrated a gradual depletion over the Holocene in their 18Odiatom values by ~3 4 , which follows the trend of decreasing summer insolation, as well as the temperature history of the Northern Hemisphere (NH), indicating a positive response of diatom oxygen isotope signal to large-scale climate changes.