1. Grassland plant community structure and function are dependent on the type, timing, frequency and intensity of disturbance. Grazing systems employing dense herds of livestock for short periods of time (e.g. Adaptive Multi-paddock Grazing; AMP) are gaining popularity as a potentially sustainable practice. Effects of AMP systems on plant diversity and composition are unknown, though theory provides some expectations. Spatially homogeneous grazing used by AMP may be a uniform ecological filter, thereby lowering plant diversity; alternatively, the AMP practice of using multiple paddocks might enhance habitat heterogeneity.Maintaining plant diversity, particularly of native species, is a key aspect of sustainability. As such, an understanding of the real-world effects of AMP grazing is needed.2. We studied grasslands within 18 pairs of ranches across the northern Great Plains. Ranches managed under AMP were paired with a neighbouring ranch (N-AMP) using regionally representative grazing practices. We collected surveys of management practices and conducted 2 years of on-farm sampling to identify plant composition and diversity.3. Ranch management practices used by self-identified AMP ranchers differed significantly (p < 0.1) from those used on neighbouring ranches, with higher stocking densities (number of animals per area at a single time) but not stocking rates (total number of animals per unit time per area) on AMP relative to N-AMP ranches.4. There were fewer plant species in AMP grasslands at both the plot and landscape scales compared to N-AMP ranches despite no overall difference in plant community composition. Management type did not alter the variability of plant community composition (beta diversity) or plot-level species evenness. Although there were trends for lower diversity of native and introduced species at both spatial scales, a significant effect was found only for native species at the landscape scale.