Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture

Zang, Shangbing; Lin, Ren‐Jang

doi:10.1007/978-1-4939-3591-8_10

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…In addition, this technique is still one of the most direct methods for demonstrating the circular configuration of circRNAs. Furthermore, the method is often integrated with more advanced procedures to investigate ribonucleoprotein complexes [ 127 ].…”

Section: Identification and Primary Characterisationmentioning

confidence: 99%

Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer

Carter

Ang

Sim

et al. 2021

ncRNA

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It is becoming increasingly evident that the non-coding genome and transcriptome exert great influence over their coding counterparts through complex molecular interactions. Among non-coding RNAs (ncRNA), long non-coding RNAs (lncRNAs) in particular present increased potential to participate in dysregulation of post-transcriptional processes through both RNA and protein interactions. Since such processes can play key roles in contributing to cancer progression, it is desirable to continue expanding the search for lncRNAs impacting cancer through post-transcriptional mechanisms. The sheer diversity of mechanisms requires diverse resources and methods that have been developed and refined over the past decade. We provide an overview of computational resources as well as proven low-to-high throughput techniques to enable identification and characterisation of lncRNAs in their complex interactive contexts. As more cancer research strategies evolve to explore the non-coding genome and transcriptome, we anticipate this will provide a valuable primer and perspective of how these technologies have matured and will continue to evolve to assist researchers in elucidating post-transcriptional roles of lncRNAs in cancer.

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Section: Identification and Primary Characterisationmentioning

confidence: 99%

Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer

Carter

Ang

Sim

et al. 2021

ncRNA

View full text Add to dashboard Cite

show abstract

Procedure for purification of recombinant preMsk1p from E. coli determines its properties as a factor of tRNA import into yeast mitochondria

Смирнова

Chicherin

Baleva

et al. 2016

Biochemistry Moscow

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Mitochondrial genomes of many eukaryotic organisms do not code for the full tRNA set necessary for organellar translation. Missing tRNA species are imported from the cytosol. In particular, one out of two cytosolic lysine tRNAs of the yeast Saccharomyces cerevisiae is partially internalized by mitochondria. The key protein factor of this process is the precursor of mitochondrial lysyl-tRNA synthetase, preMsk1p. In this work, we show that recombinant preMsk1p purified from E. coli in native conditions, when used in an in vitro tRNA import system, demonstrates some properties different from those shown by the renatured protein purified from E. coli in the denatured state. We also discuss the possible mechanistic reasons for this phenomenon.

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Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture

Cited by 2 publications

References 14 publications

Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer

Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer

Procedure for purification of recombinant preMsk1p from E. coli determines its properties as a factor of tRNA import into yeast mitochondria

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