Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

Břı́za, J.; Pavingerová, D.; Vlasák, Josef; Niedermeierová, H.

doi:10.18388/abp.2013_1998

Cited by 11 publications

(4 citation statements)

References 30 publications

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“…For example, transgenic rapeseed containing the Cry1Ac gene can control diamondback moth, hairy bug, and cotton bollworm [ 19 ], and transgenic pigeon bean containing the Cry2Aa gene is resistant to pod borers [ 20 ]. The Cry3A gene in transgenic spruce showed toxicity to spruce bark beetles, indicating that Bt and its toxin proteins add significant value to biological control [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Zhao

Ali

Wang

et al. 2022

IJMS

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The plant parasitic nematode, Aphelenchoides besseyi, is a serious pest causing severe damage to various crop plants and vegetables. The Bacillus thuringiensis (Bt) strains, GBAC46 and NMTD81, and the biological strain, FZB42, showed higher nematicidal activity against A. besseyi, by up to 88.80, 82.65, and 75.87%, respectively, in a 96-well plate experiment. We screened the whole genomes of the selected strains by protein-nucleic acid alignment. It was found that the Bt strain GBAC46 showed three novel crystal proteins, namely, Cry31Aa, Cry73Aa, and Cry40ORF, which likely provide for the safe control of nematodes. The Cry31Aa protein was composed of 802 amino acids with a molecular weight of 90.257 kDa and contained a conserved delta-endotoxin insecticidal domain. The Cry31Aa exhibited significant nematicidal activity against A. besseyi with a lethal concentration (LC50) value of 131.80 μg/mL. Furthermore, the results of in vitro experiments (i.e., rhodamine and propidium iodide (PI) experiments) revealed that the Cry31Aa protein was taken up by A. besseyi, which caused damage to the nematode’s intestinal cell membrane, indicating that the Cry31Aa produced a pore-formation toxin. In pot experiments, the selected strains GBAC46, NMTD81, and FZB42 significantly reduced the lesions on leaves by up to 33.56%, 45.66, and 30.34% and also enhanced physiological growth parameters such as root length (65.10, 50.65, and 55.60%), shoot length (68.10, 55.60, and 59.45%), and plant fresh weight (60.71, 56.45, and 55.65%), respectively. The number of nematodes obtained from the plants treated with the selected strains (i.e., GBAC46, NMTD81, and FZB42) and A. besseyi was significantly reduced, with 0.56, 0.83., 1.11, and 5.04 seedling mL−1 nematodes were achieved, respectively. Moreover, the qRT-PCR analysis showed that the defense-related genes were upregulated, and the activity of hydrogen peroxide (H2O2) increased while malondialdehyde (MDA) decreased in rice leaves compared to the control. Therefore, it was concluded that the Bt strains GBAC46 and NMTD81 can promote rice growth, induce high expression of rice defense-related genes, and activate systemic resistance in rice. More importantly, the application of the novel Cry31Aa protein has high potential for the efficient and safe prevention and green control of plant parasitic nematodes.

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Section: Introductionmentioning

confidence: 99%

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Zhao

Ali

Wang

et al. 2022

IJMS

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“…Bt toxins are also being used in generating trangenic crops effectively control crops pests such as CryIAc in rapeseed to control hairy bugs, diamondback moths, and cotton bollworms [87]. Cry2Aagene in transgenic pigeon beans to control pod borers [88], Cry3A gene in transgenic spruce to control bark beetles [89]. According to a recent report, a significant change in the amount of herbicides and pesticide application was observed in the USA with the adoption of herbicides tolerant GM plants [90], such as; transgenic soybean [91], summer corn and cotton.…”

Section: Transgenic Approaches For Environmental Protectionmentioning

confidence: 99%

Assessment of Benefits and Risk of Genetically Modified Plants and Products: Current Controversies and Perspective

Ghimire

Kim

et al. 2023

Sustainability

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Genetic transformation has emerged as an important tool for the genetic improvement of valuable plants by incorporating new genes with desirable traits. These strategies are useful especially in crops to increase yields, disease resistance, tolerance to environmental stress (cold, heat, drought, salinity, herbicides, and insects) and increase biomass and medicinal values of plants. The production of healthy plants with more desirable products and yields can contribute to sustainable development goals. The introduction of genetically modified food into the market has raised potential risks. A proper assessment of their impact on the environment and biosafety is an important step before their commercialization. In this paper, we summarize and discuss the risks and benefits of genetically modified plants and products, human health hazards by genetically transformed plants, environmental effects, Biosafety regulations of GMO foods and products, and improvement of medicinal values of plants by the genetic transformation process. The mechanisms of action of those products, their sources, and their applications to the healthcare challenges are presented. The present studies pointed out the existence of several controversies in the use of GMOs, mainly related to the human health, nutritions, environmental issues. Willingness to accept genetically modified (GM) products and the adoption of biosafety regulations varies from country to country. Knowledge about the gene engineering technology, debate between the government agencies, scientist, environmentalist and related NGOs on the GM products are the major factors for low adoptions of biosafety regulation. Therefore, the genetic transformation will help in the advancement of plant species in the future; however, more research and detailed studies are required.

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“…Currently, several reports concerning the genetic transformation of P. abies embryogenic cultures are available [133][134][135][136]. Most studies were carried out on embryogenic tissues using Agrobacterium tumefaciens-mediated transformation.…”

Section: Se-the Future Of Forestry? 61 Applied Biotechnology and Sementioning

confidence: 99%

“…For example, Wenck et al [133] obtained over 100 transformed embryogenic cell lines after their co-cultivation with Agrobacterium strains containing additional copies of virulence genes (either a constitutively active virG or extra copies of virG and virB, both from pTiBo542). Additionally, Briža et al [136] reported 70 transgenic lines, which were transformed with Agrobacterium tumefaciens. In this experiment the modified versions of Cry3A gene of Bacillus thuringiensis, with the increased toxicity against spruce bark beetle, were introduced into embryogenic tissues.…”

Section: Se-the Future Of Forestry? 61 Applied Biotechnology and Sementioning

confidence: 99%

Somatic Embryogenesis of Norway Spruce and Scots Pine: Possibility of Application in Modern Forestry

Hazubska-Przybył

Wawrzyniak

Kijowska‐Oberc

et al. 2022

Forests

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Somatic embryogenesis (SE) is an important method for the vegetative propagation of trees. SE is the developmental in vitro process in which embryos are produced from somatic cells. This method can be integrated with other biotechnological techniques, genomic breeding and cryopreservation, which enables commercial-scale sapling production of selected high-yielding genotypes in wood production combined with fast breeding cycles. The SE is potential tool to improve plant stock in comparison with seed orchards. It can be useful for ecologically and economically important species, such as Norway spruce (Picea abies L. Karst.) and Scots pine (Pinus sylvestris L.), ensuring stable production in the era of climate change and biodiversity crisis. In this review, we summarize the current state of research on problems associated with somatic embryogenesis in P. abies and P. sylvestris.

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Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

Cited by 11 publications

References 30 publications

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Toxicity of Bacillus thuringiensis Strains Derived from the Novel Crystal Protein Cry31Aa with High Nematicidal Activity against Rice Parasitic Nematode Aphelenchoides besseyi

Assessment of Benefits and Risk of Genetically Modified Plants and Products: Current Controversies and Perspective

Somatic Embryogenesis of Norway Spruce and Scots Pine: Possibility of Application in Modern Forestry

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