Nosocomial bacterial infections in Intensive Care Units. I: Organisms and mechanisms of antibiotic resistance

Lim, Suk-Kyung; Webb, Steven A R

doi:10.1111/j.1365-2044.2005.04220.x

Cited by 79 publications

(52 citation statements)

References 126 publications

(289 reference statements)

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“…Development and dissemination of antibiotic-resistance genes is a serious problem in the treatment of infectious diseases (Goossens, 2005;Lim & Webb, 2005). An important step in coping with this threat is to elucidate and to understand pathways for resistance gene spread.…”

Section: Introductionmentioning

confidence: 99%

Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics

et al. 2009

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To detect plasmid-borne antibiotic-resistance genes in wastewater treatment plant (WWTP) bacteria, 192 resistance-gene-specific PCR primer pairs were designed and synthesized. Subsequent PCR analyses on total plasmid DNA preparations obtained from bacteria of activated sludge or the WWTP's final effluents led to the identification of, respectively, 140 and 123 different resistance-gene-specific amplicons. The genes detected included aminoglycoside, blactam, chloramphenicol, fluoroquinolone, macrolide, rifampicin, tetracycline, trimethoprim and sulfonamide resistance genes as well as multidrug efflux and small multidrug resistance genes. Some of these genes were only recently described from clinical isolates, demonstrating genetic exchange between clinical and WWTP bacteria. Sequencing of selected resistance-genespecific amplicons confirmed their identity or revealed that the amplicon nucleotide sequence is very similar to a gene closely related to the reference gene used for primer design. These results demonstrate that WWTP bacteria are a reservoir for various resistance genes. Moreover, detection of about 64 % of the 192 reference resistance genes in bacteria obtained from the WWTP's final effluents indicates that these resistance determinants might be further disseminated in habitats downstream of the sewage plant.

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Section: Introductionmentioning

confidence: 99%

Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics

et al. 2009

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“…Significant among these elements are various classes of plasmids, transposons, and integrons which may lack resistance determinants or carry one or multiple determinants. Resistance determinants that have become globally dispersed in environmental and clinically significant bacteria include mercury(II) resistance (2,17), evident even in ancient bacteria (27), and antibiotic resistance, which has increased in dominance since the advent of the antibiotic era (23,40). This paper concerns the mercury resistance (mer) transposons Tn502 and Tn512, whose sequence organization and transpositional behavior show that they are new members of a family of elements exemplified by the mer transposon Tn5053 (22).…”

mentioning

confidence: 99%

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Petrovski

Stanisich

2010

J Bacteriol

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In this study, we report on the transposition behavior of the mercury(II) resistance transposons Tn502 and Tn512, which are members of the Tn5053 family. These transposons exhibit targeted and oriented insertion in the par region of plasmid RP1, since par-encoded components, namely, the ParA resolvase and its cognate res region, are essential for such transposition. Tn502 and, under some circumstances, Tn512 can transpose when par is absent, providing evidence for an alternative, par-independent pathway of transposition. We show that the alternative pathway proceeds by a two-step replicative process involving random target selection and orientation of insertion, leading to the formation of cointegrates as the predominant product of the first stage of transposition. Cointegrates remain unresolved because the transposon-encoded (TniR) recombination system is relatively inefficient, as is the host-encoded (RecA) system. In the presence of the res-ParA recombination system, TniR-mediated (and RecA-mediated) cointegrate resolution is highly efficient, enabling resolution both of cointegrates involving functional transposons (Tn502 and Tn512) and of defective elements (In0 and In2). These findings implicate the target-encoded accessory functions in the second stage of transposition as well as in the first. We also show that the par-independent pathway enables the formation of deletions in the target molecule.

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“…Staphylococcus aureus gastroenteritis is one of the most prevalent foodborne bacterial intoxications worldwide (Jablonski & Bohach 2001;Stewart 2005). Furthermore, staphylococci resistant to beta-lactam antibiotics are among the prominent nosocomial pathogens (Lim & Webb 2005). Antimicrobial resistance represents a global important public health concern (Harrison & Lederberg 1998;Finch & Hunter 2006).…”

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confidence: 99%

Prevalence, distribution, and antimicrobial susceptibility of Staphylococcus aureus in ready-to-eat salads and in the environment of a salad manufacturing plant in Northern Greece

Sergelidis¹,

Abrahim²,

Anagnostou³

et al. 2012

Czech J. Food Sci.

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Sergelidis D., Abrahim A., Anagnostou V., Govaris A., Papadopoulos T., Papa A. (2012): Prevalence, distribution, and antimicrobial susceptibility of Staphylococcus aureus in ready-to-eat salads and in the environment of a salad manufacturing plant in Northern Greece. Czech J. Food Sci., 30: 285-291.The prevalence, distribution, and antibiotic susceptibility of Staphylococcus aureus was investigated in ready-to-eat (RTE) salads, the environment, and the personnel of a salad producing plant in Greece. Staphylococci were isolated from 27% of the samples. Apart from three isolates which were sensitive to all antibiotics, all the others exhibited resistance from two up to five antibiotics. None of the isolates was resistant to oxacillin. Random Amplified Polymorphic DNA (RAPD) analysis showed no genetic relation between the human and salad isolates while one RAPD clone of S. aureus predominated among the salad samples. The results suggest that an effective application of Good Manufacturing Practices (GMP) is needed along the food production chain to prevent the contamination of RTE foods.

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Nosocomial bacterial infections in Intensive Care Units. I: Organisms and mechanisms of antibiotic resistance

Cited by 79 publications

References 126 publications

Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics

Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Prevalence, distribution, and antimicrobial susceptibility of Staphylococcus aureus in ready-to-eat salads and in the environment of a salad manufacturing plant in Northern Greece

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