Nosocomial dissemination of Providencia stuartii isolates carrying blaOXA-48, blaPER-1, blaCMY-4 and qnrA6 in a Tunisian hospital

Mnif, Basma; Ktari, Sonia; Chaari, Anis; Medhioub, Fatma; Rhimi, Faouzia; Bouaziz, Mounir; Hammami, Adnane

doi:10.1093/jac/dks386

Cited by 38 publications

(22 citation statements)

References 12 publications

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“…TEM types, especially TEM-52 and TEM-92, dominate in France and Italy (44,45), while CTX-M types are common in Israel (CTX-M-2), Poland (CTX-M-3), Japan (CTX-M-2), South Korea (CTX-M-14), and Argentina (CTX-M-2) (41,42,(46)(47)(48). Providencia species producing ESBL types other than CTX-M-2 have only been reported on a few occasions (49).…”

Section: Discussionmentioning

confidence: 99%

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Hansen

Bortolaia

Damborg

et al. 2014

Appl Environ Microbiol

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, resulting in 98 isolates identified by matrixassisted laser desorption ionization-time of flight mass spectrometry and tested for bla CTX-M . CTX-M-positive isolates (n ‫؍‬ 86) were typed by pulsed-field gel electrophoresis (PFGE). Plasmids harboring bla CTX-M were characterized in 22 representative isolates by replicon typing and restriction fragment length polymorphism. Based on the PFGE results, all individuals shed unrelated CTX-M-14-producing E. coli strains during the course of life. Concomitant shedding of CTX-M-2/97-producing Proteus mirabilis or Providencia rettgeri was observed in two sows and two offspring. At least two genetically unrelated CTX-M-producing E. coli strains were isolated from approximately one-fourth of the samples, with remarkable differences between isolates obtained by enrichment and direct plating. A clear decrease in strain diversity was observed after weaning. Dissemination of bla CTX-M-14 within the farm was attributed to horizontal transfer of an IncK plasmid that did not carry additional resistance genes and persisted in the absence of antimicrobial selective pressure. Assessment of strain diversity was shown to be influenced by the production stage from which samples were collected, as well as by the isolation method, providing useful information for the design and interpretation of future epidemiological studies of CTX-M-producing Enterobacteriaceae in pig farms.

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Section: Discussionmentioning

confidence: 99%

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Hansen

Bortolaia

Damborg

et al. 2014

Appl Environ Microbiol

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“…The results revealed that bla PER-1 was located on a 160-kb broad-host-range IncA/C plasmid within the RJ354 isolate and its transconjugant. In previous studies, bla PER-1 was also found to be located on IncA/C plasmids in Providencia stuartii and Klebsiella pneumoniae strains from Tunisia and South Korea, respectively (10,13), indicating that the IncA/C plasmids may play a role in the dissemination of bla PER-1 .…”

mentioning

confidence: 85%

Molecular Characterization of IS CR1 -Mediated bla _PER-1 in a Non-O1, Non-O139 Vibrio cholerae Strain from China

Xie

Zhang

et al. 2015

Antimicrob Agents Chemother

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bWe report the detection of PER-1 extended-spectrum ␤-lactamase (ESBL) in a clinical non-O1, non-O139 Vibrio cholerae strain from China. ISCR1-mediated bla PER-1 was embedded in a complex In4 family class 1 integron belonging to the lineage of Tn1696 on a conjugative IncA/C plasmid. A free 8.98-kb circular molecule present with the ISCR1-bla PER-1 -truncated 3=-conserved sequence (CS) structure was detected in this isolate. These findings may provide insight into the mobilization of bla PER-1 .

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“…More concerning, however, was a retrospective analysis that uncovered an outbreak of OXA-48-producing Enterobacteriaceae in a Dutch hospital that had been ongoing for 2 years (53). OXA-48 has disseminated to a wide variety of Enterobacteriaceae species, including Klebsiella spp., E. coli, Citrobacter spp., Serratia marcescens (54)(55)(56), Enterobacter spp., Morganella morganii (55), Providencia stuartii (57), Raoultella planticola (56), and Salmonella enterica (51).…”

Section: Current Status Of Carbapenemasesmentioning

confidence: 99%

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

et al. 2016

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SUMMARYCarbapenemases have become a significant mechanism for broad-spectrum β-lactam resistance inEnterobacteriaceaeand other Gram-negative bacteria such asPseudomonasandAcinetobacterspp. Intestinal carriage of carbapenemase-producing organisms (CPOs) is an important source of transmission. Isolation of carriers is one strategy that can be used to limit the spread of these bacteria. In this review, we critically examine the clinical performance, advantages, and disadvantages of methods available for the detection of intestinal carriage of CPOs. Culture-based methods (Centers for Disease Control and Prevention [CDC] protocols, chromogenic media, specialized agars, and double-disk synergy tests) for detecting carriage of CPOs are convenient due to their ready availability and low cost, but their limited sensitivity and long turnaround time may not always be optimal for infection control practices. Contemporary nucleic acid amplification techniques (NAATs) such as real-time PCR, hybridization assays, loop-mediated isothermal amplification (LAMP), or a combined culture and NAAT approach may provide fast results and/or added sensitivity and specificity compared with culture-based methods. Infection control practitioners and clinical microbiologists should be aware of the strengths and limitations of available methods to determine the most suitable approach for their medical facility to fit their infection control needs.

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Nosocomial dissemination of Providencia stuartii isolates carrying blaOXA-48, blaPER-1, blaCMY-4 and qnrA6 in a Tunisian hospital

Abstract: This is the first report of an outbreak due to P. stuartii isolates carrying bla(OXA-48) in Tunisia. The simultaneous expression of various resistance genes (bla(OXA-48), bla(CMY-4), bla(PER-1), qnrA and aac-6'-Ib) by P. stuartii isolates is alarming.

Cited by 38 publications

References 12 publications

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Molecular Characterization of IS CR1 -Mediated bla _PER-1 in a Non-O1, Non-O139 Vibrio cholerae Strain from China

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

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Nosocomial dissemination of Providencia stuartii isolates carrying blaOXA-48, blaPER-1, blaCMY-4 and qnrA6 in a Tunisian hospital

Abstract: This is the first report of an outbreak due to P. stuartii isolates carrying bla(OXA-48) in Tunisia. The simultaneous expression of various resistance genes (bla(OXA-48), bla(CMY-4), bla(PER-1), qnrA and aac-6'-Ib) by P. stuartii isolates is alarming.

Cited by 38 publications

References 12 publications

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Molecular Characterization of IS CR1 -Mediated bla PER-1 in a Non-O1, Non-O139 Vibrio cholerae Strain from China

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

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Molecular Characterization of IS CR1 -Mediated bla _PER-1 in a Non-O1, Non-O139 Vibrio cholerae Strain from China