Nosocomial infections due to serratia marcescens — Clinical findings, antibiotic susceptibility patterns and fine typing

Bollmann, R.; Halle, E.; Sokolowska-Köhler, W; El, Grauel; Buchholz, P; Klare, Ingo; Tschäpe, Helmut; Witte, Wolfgang

doi:10.1007/bf01650711

Cited by 25 publications

(17 citation statements)

References 20 publications

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“…Increased resistance to tetracycline occurred independently in four subclades across the phylogenetic tree and was linked with the presence of efflux pumps tetA5 and tetA3, both of which have been reported as resistance determinants (Supplemental Fig. S6; Bollmann et al 1989). The tetA5 gene also accounted for the elevated resistance to minocycline in 16 samples and appears to be inserted within the chromosome (Table 1).…”

Section: Resultsmentioning

confidence: 82%

Recent independent emergence of multiple multidrug-resistantSerratia marcescensclones within the United Kingdom and Ireland

et al. 2016

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Serratia marcescens, a member of the Enterobacteriaceae family, is a Gram-negative bacterium responsible for a wide range of nosocomial infections. The emergence of multidrug-resistant strains is an increasing danger to public health. To design effective means to control the dissemination of S. marcescens, an in-depth analysis of the population structure and variation is required. Utilizing whole-genome sequencing, we characterized the population structure and variation, as well as the antimicrobial resistance determinants, of a systematic collection of antimicrobial-resistant S. marcescens associated with bloodstream infections in hospitals across the United Kingdom and Ireland between 2001 and 2011. Our results show that S. marcescens is a diverse species with a high level of genomic variation. However, the collection was largely composed of a limited number of clones that emerged from this diverse background within the past few decades. We identified potential recent transmissions of these clones, within and between hospitals, and showed that they have acquired antimicrobial resistance determinants for different beta-lactams, ciprofloxacin, and tetracyclines on multiple occasions. The expansion of these multidrug-resistant clones suggests that the treatment of S. marcescens infections will become increasingly difficult in the future.

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Section: Resultsmentioning

confidence: 82%

Recent independent emergence of multiple multidrug-resistantSerratia marcescensclones within the United Kingdom and Ireland

et al. 2016

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“…As in outbreaks that have occurred in adults, genotyping methods have been used in many pediatric outbreaks to type the involved S. marcescens strains, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of disrupted S. marcescens cells (116), plasmid profiling (18,41,157,258), PFGE (52,190,228,239,269,270,309,313,338,341,366,396), ribotyping (150), rep-PCR (239, 393), RAPD-PCR (18), and PCR fingerprinting (366). Voelz and others performed a systematic analysis of several pediatric S. marcescens outbreak studies from 1984 to 2010 that utilized typing procedures to determine clonality.…”

Section: S Marcescensmentioning

confidence: 99%

“…Many nosocomial outbreaks in both pediatric and adult patients have occurred with S. marcescens strains resistant to one or more aminoglycosides (17,41,53,79,88,93,120,238,258,280,285,287,339,356,423). Most of the initial reports of aminoglycoside-resistant S. marcescens nosocomial outbreaks occurred in the mid-to late 1970s (for example, see references 79, 93, and 339).…”

Section: Aminoglycoside Resistance In Serratiamentioning

confidence: 99%

“…In addition, the RND pump SdeAB was shown to provide an increase in tetracycline resistance after S. marcescens was exposed to cetylpyridinium chloride (255). Also, a tetracyclinespecific efflux pump, encoded by tetA (41), was identified in an These genes all code for efflux pumps. Tetracycline and minocycline are substrates for TetB, but the other pumps primarily transport tetracycline (73).…”

Section: Resistance To the Tetracyclines In Serratia Speciesmentioning

confidence: 99%

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Serratia Infections: from Military Experiments to Current Practice

2011

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SUMMARY Serratia species, in particular Serratia marcescens , are significant human pathogens. S. marcescens has a long and interesting taxonomic, medical experimentation, military experimentation, and human clinical infection history. The organisms in this genus, particularly S. marcescens , were long thought to be nonpathogenic. Because S. marcescens was thought to be a nonpathogen and is usually red pigmented, the U.S. military conducted experiments that attempted to ascertain the spread of this organism released over large areas. In the process, members of both the public and the military were exposed to S. marcescens , and this was uncovered by the press in the 1970s, leading to U.S. congressional hearings. S. marcescens was found to be a certain human pathogen by the mid-1960s. S. marcescens and S. liquefaciens have been isolated as causative agents of numerous outbreaks and opportunistic infections, and the association of these organisms with point sources such as medical devices and various solutions given to hospitalized patients is striking. Serratia species appear to be common environmental organisms, and this helps to explain the large number of nosocomial infections due to these bacteria. Since many nosocomial infections are caused by multiply antibiotic-resistant strains of S. marcescens , this increases the danger to hospitalized patients, and hospital personnel should be vigilant in preventing nosocomial outbreaks due to this organism. S. marcescens , and probably other species in the genus, carries several antibiotic resistance determinants and is also capable of acquiring resistance genes. S. marcescens and S. liquefaciens are usually identified well in the clinical laboratory, but the other species are rare enough that laboratory technologists may not recognize them. 16S rRNA gene sequencing may enable better identification of some of the less common Serratia species.

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“…Serratia marcescens is a recognized nosocomial pathogen that causes pneumonia, septicemia, meningitis, and urinary tract infections (1,7). S. marcescens N28b (O4) produces a bacteriocin able to kill E. coli K-12 (48); this bacteriocin binds to the core of LPS and to the outer membrane proteins OmpA and OmpF of sensitive E. coli cells (11).…”

mentioning

confidence: 99%

Genetic and Structural Characterization of the Core Region of the Lipopolysaccharide from Serratia marcescens N28b (Serovar O4)

Coderch

Piqué

Lindner³

et al. 2004

J Bacteriol

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The gene cluster (waa) involved in Serratia marcescens -Glc-(136)-␣-Glc-(134))-␣-D-GlcN-(134)-␣-D-GalA-[(241)-␣-D,D-Hep-(241)-␣-Hep]-(133)-␣-L,D-Hep[(741)-␣-L,D-Hep]-(133)-␣-L,D-Hep-[(441)-␤-D-Glc]-(135)-Kdo.The D configuration of the ␤-Glc, ␣-GclN, and ␣-GalA residues was deduced from genetic data and thus is tentative. Furthermore, other oligosaccharides were identified by ion cyclotron resonance-Fourier-transformed electrospray ionization mass spectrometry, which presumably contained in addition one residue of D-glycero-D-talooct-2-ulosonic acid (Ko) or of a hexuronic acid. Several ions were identified that differed from others by a mass of ؉80 Da, suggesting a nonstoichiometric substitution by a monophosphate residue. However, none of these molecular species could be isolated in substantial amounts and structurally analyzed. On the basis of the structure shown above and the analysis of nonpolar mutants, functions are suggested for the genes involved in core biosynthesis.

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Nosocomial infections due to serratia marcescens — Clinical findings, antibiotic susceptibility patterns and fine typing

Cited by 25 publications

References 20 publications

Recent independent emergence of multiple multidrug-resistantSerratia marcescensclones within the United Kingdom and Ireland

Recent independent emergence of multiple multidrug-resistantSerratia marcescensclones within the United Kingdom and Ireland

Serratia Infections: from Military Experiments to Current Practice

Genetic and Structural Characterization of the Core Region of the Lipopolysaccharide from Serratia marcescens N28b (Serovar O4)

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