Nosocomial Infections Occurring during Receipt of Circulatory Support with the Paracorporeal Ventricular Assist System

Mekontso-Dessap, Armand; Kirsch, Matthias; Vermès, Emmanuelle; Brun‐Buisson, Christian; Loisance, D; Houël, Rémi

doi:10.1086/343825

Cited by 16 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a current case series, 62 85% of LVAD infections occurred when the device was left in place for Ͼ2 weeks. The incidence of infection has been highly variable among different surveys [63][64][65][66][67][68][69][70][71][72][73][74][75] and has been reported to range between 13% and 80%. The wide variability in infection risk is, in part, due to different types of infections that have been included under the category of LVAD-related infections.…”

Section: Left Ventricular Assist Devicesmentioning

confidence: 99%

Nonvalvular Cardiovascular Device–Related Infections

Baddour¹,

Bettmann²,

Bolger³

et al. 2003

Circulation

447

326

View full text Add to dashboard Cite

Section: Left Ventricular Assist Devicesmentioning

confidence: 99%

Nonvalvular Cardiovascular Device–Related Infections

Baddour¹,

Bettmann²,

Bolger³

et al. 2003

Circulation

447

326

View full text Add to dashboard Cite

“…S. epidermidis and other CoNS are common members of the normal flora of skin and mucous membranes and are therefore common contaminants in culture, particularly in blood cultures. A conundrum often exists in the interpretation of cultures that contain these microorganisms, since CoNS have also become an important cause of hospital-acquired infections (5,9,21). It is most important to distinguish between S. aureus and CoNS in clinical samples, given the differences in virulence between these organisms (2,28,29).…”

mentioning

confidence: 99%

Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets

Sakai

Procop²,

Kobayashi

et al. 2004

J Clin Microbiol

View full text Add to dashboard Cite

A real-time PCR assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. One probe set detects the Staphylococcus genus, whereas the other probe set is specific for Staphylococcus aureus. One hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. This assay was 100% sensitive and 100% specific for the detection of the Staphylococcus genus and of S. aureus.The genus Staphylococcus currently consists of 38 species (14, 15). Among staphylococci, Staphylococcus aureus is the most virulent species and the most important pathogen, but the incidence of infections caused by coagulase-negative staphylococci (CoNS) has increased throughout the world (2,28,29). S. epidermidis and other CoNS are common members of the normal flora of skin and mucous membranes and are therefore common contaminants in culture, particularly in blood cultures. A conundrum often exists in the interpretation of cultures that contain these microorganisms, since CoNS have also become an important cause of hospital-acquired infections (5, 9, 21). It is most important to distinguish between S. aureus and CoNS in clinical samples, given the differences in virulence between these organisms (2,28,29). It is also important, however, to confirm the presence of CoNS given the increasing importance of CoNS infections (27,33).Phenotypic methods have been the standard means of differentiating S. aureus from CoNS and for differentiating the members of CoNS. These methods all require growth of the organisms followed by biochemical testing, are time-consuming, and may still occasionally produce inaccurate results (7,8,23,26). A variety of genotypic methods have been explored for the rapid detection of S. aureus and/or for the detection and differentiation of CoNS. These methods include the use of traditional in situ hybridization, peptide-nucleic acid in situ hybridization, and a variety of nucleic acid amplification-based methods (1,3,11,13,17,18,22).We designed a single broad-range PCR primer set directed toward the tuf gene to amplify the most clinically relevant staphylococci. For this study, PCR products were detected by use of a LightCycler instrument (Roche Diagnostics, Indianapolis, Ind.) and two sets of fluorescence resonance energy transfer (FRET) hybridization probes. The first set of FRET hybridization probes was designed to detect a signature sequence of the Staphylococcus genus, whereas the second set of FRET hybridization probes was designed to specifically detect S. aureus. This assay can detect S. aureus and CoNS simultaneously and can differentiate S. aureus by a postamplification melt curve analysis using the F3 channel of the LightCycler system.We validated this dual-FRET-probe real-time PCR assay for Staphylococcus with lysates from 138 cultivated bacterial reference strains and well-characterized clinical isolates...

show abstract

“…The organisms that are most frequently responsible for VAD-related infections are Staphylococcus species, Pseudomonas aeruginosa, and Candida. 20 The risk factors for infection among the patients with VAD relate to patient comorbidities especially diabetes mellitus, obesity, and chronic obstructive pulmonary disease, as well as other factors such as length of the preoperative hospital stay, postoperative bleeding, blood transfusions, and the need for surgical re-exploration. 20 There are multiple studies that report high rates of the device-related infections.…”

Section: Infectionmentioning

confidence: 99%

“…20 The risk factors for infection among the patients with VAD relate to patient comorbidities especially diabetes mellitus, obesity, and chronic obstructive pulmonary disease, as well as other factors such as length of the preoperative hospital stay, postoperative bleeding, blood transfusions, and the need for surgical re-exploration. 20 There are multiple studies that report high rates of the device-related infections. The International Society for Heart and Lung Transplantation's Mechanical Circulatory Support Database reports 32.5% of devicerelated infections that lead to 7.9% mortality rate among VAD recipients.…”

Section: Infectionmentioning

confidence: 99%

Complications in Patients With Ventricular Assist Devices

Barnes

2008

Dimensions of Critical Care Nursing

View full text Add to dashboard Cite

Heart failure affects more than 5 million people in the United States. The treatments of this disease include medical therapy, heart transplantation, and the implantation of ventricular assist devices. These devices are used in patients who are no longer responsive to conservative medical treatment, who are not candidates for a heart transplantation (destination therapy), who are awaiting a heart transplantation (bridge to transplantation), and who have acute heart failure and whose myocardial function is expected to return to normal (bridge to recovery). Although this therapy improves the mortality and quality of life among patients with heart failure, the devices also carry risk of multiple complications. This article discusses the acute and long-term complications of ventricular assist devices.

show abstract

Nosocomial Infections Occurring during Receipt of Circulatory Support with the Paracorporeal Ventricular Assist System

Cited by 16 publications

References 31 publications

Nonvalvular Cardiovascular Device–Related Infections

Nonvalvular Cardiovascular Device–Related Infections

Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets

Complications in Patients With Ventricular Assist Devices

Contact Info

Product

Resources

About