Nosocomial spread of multidrug-resistant group B streptococci with reduced penicillin susceptibility belonging to clonal complex 1

Abstract

This is the first description of the nosocomial spread of multidrug-resistant PRGBS strains belonging to the genetic lineage ST458.

Serotype distribution and antimicrobial susceptibilities of Streptococcus agalactiae isolated from infected cultured tilapia (Oreochromis niloticus) in Thailand: Nine-year perspective

“…Multiple drug resistance patterns of GBS have been reported in several organisms, including fish (Duremdez et al, 2004) and humans (Berkowitz et al, 1990;Nagano et al, 2012;Banno et al, 2014). Further characterization of fluoroquinolone and clindamycin and resistance to other drugs by molecular methods is needed to confirm that these oxytetracycline-resistant GBS isolates are multi-drug-resistant.…”

Characterization of Multidrug-Resistant Group B Streptococci with Reduced Penicillin Susceptibility Forming Small Non-Beta-Hemolytic Colonies on Sheep Blood Agar Plates

“…Indeed, an event of probable nosocomial spread of multidrug-resistant PRGBS isolates has been previously reported (8). However, the possibility of direct contact between the two inpatients from whom the PRGBS isolates were recovered was low because they were admitted to different wards of the hospital.…”

“…Moreover, these isolates harbored five amino acid substitutions in PBP2X, including V405A, which is frequently observed in PRGBS (3). In addition, these isolates harbored several amino acid substitutions in the quinolone resistance-determining regions of GyrA, GyrB, and ParC, which were reported to be responsible for fluoroquinolone resistance in GBS (8,11). Furthermore, an erm(B) gene, which confers resistance to macrolides and lincosamides (12,13), was detected by PCR (see Table S1 in the supplemental material).…”

Penicillin-Susceptible Group B Streptococcal Clinical Isolates with Reduced Cephalosporin Susceptibility

“…Ceftizoxime MICs were determined by Etest according to the manufacturer's instructions (Sysmex bioMérieux Co., Ltd., Tokyo, Japan). Sequence analyses of the pbp2x, -2b, and -1a genes were performed as previously described (8,16). Pulsed-field gel electrophoresis (PFGE) of chromosomal digests with SmaI was performed as described previously by Nagano et al (8).…”