Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines

Zhang, Haihong; Xi, Hongqing; Cai, Aizhen; Xia, Qi-Jun; Wang, Xin Xin; Lu, Canrong; Zhang, Yong; Zhang, Song; Wang, Hua; Li, Qinfang; Chen, Lin; Guo, Zi-Kuan

doi:10.1007/s10620-012-2330-1

Cited by 23 publications

(22 citation statements)

References 26 publications

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“…Three human gastric cancer cell lines were used in this study,two CD44-positive cell lines (BGC-823 and SGC-7901) (Xue et al 2012;Zhang et al 2013) and one CD44-negative cell line (MKN-28) (Yokozaki 2000). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

A CD44 specific peptide developed by phage display for targeting gastric cancer

et al. 2015

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Section: Resultsmentioning

confidence: 99%

A CD44 specific peptide developed by phage display for targeting gastric cancer

et al. 2015

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“…Zhange et al [47] showed CSC properties in SP cells from the MKN-45 cell line but not the BGC-823 cell line. Although some studies in gastric cancer lines have shown CSC properties in the SP [48,49], others have found no difference compared to a non-SP subset [11,50,51].…”

Section: Side Population Assaymentioning

confidence: 99%

Gastric cancer stem cells: evidence, potential markers, and clinical implications

et al. 2015

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Gastric cancer is a significant global health problem. It is the fifth most common cancer and third leading cause of cancer-related death worldwide (Torre et al. in CA Cancer J Clin 65(2):87-108, 2015). Despite advances in treatment, overall prognosis remains poor, due to tumour relapse and metastasis. There is an urgent need for novel therapeutic approaches to improve clinical outcomes in gastric cancer. The cancer stem cell (CSC) model has been proposed to explain the high rate of relapse and subsequent resistance of cancer to current systemic treatments (Vermeulen et al. in Lancet Oncol 13(2):e83-e89, 2012). CSCs have been identified in many solid malignancies, including gastric cancer, and have significant clinical implications, as targeting the CSC population may be essential in preventing the recurrence and spread of a tumour (Dewi et al. in J Gastroenterol 46(10):1145-1157, 2011). This review seeks to summarise the current evidence for CSC in gastric cancer, with an emphasis on candidate CSC markers, clinical implications, and potential therapeutic approaches.

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“…In addition, the growth kinetics of SP cells does not differ from those of the non-SP cells [153]. SP cells from the gastric cancer BGC-823 cell line also do not behave as CSCs do [154]. However, some studies have yielded conflicting results.…”

Section: Side Population (Sp) Cellsmentioning

confidence: 99%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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Cancer stem cells (CSCs) constitute a subpopulation of cancer cells that have the potential for self-renewal, multipotent differentiation, and tumorigenicity. Studies on CSC biology and CSC-targeted therapies depend on CSC isolation and/or enrichment methodologies. Scientists have conducted extensive research in this field since John Dick's group successfully isolated CSCs based on the expression of the CD34 and CD38 surface markers. Progress in CSC research has been greatly facilitated by the enrichment and isolation of these cells. In this review, we summarize the current strategies used in our and other laboratories for CSC isolation and enrichment, including methods based on stem cell surface markers, intracellular enzyme activity, the concentration of reactive oxygen species, the mitochondrial membrane potential, promoter-driven fluorescent protein expression, autofluorescence, suspension/adherent culture, cell division, the identification of side population cells, resistance to cytotoxic compounds or hypoxia, invasiveness/adhesion, immunoselection, and physical property. Although many challenges remain to be overcome, it is reasonable to believe that more reliable, efficient, and convenient methods will be developed in the near future.

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Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines

Abstract: SP cells from MKN-45 possess cancer stem cell properties and proved that they were gastric cancer stem-like cells. SP cells from BGC-823 didn't possess cancer stem cell properties and proved that not all SP cells contain cancer stem-like cells in gastric cancer cell lines.

Cited by 23 publications

References 26 publications

A CD44 specific peptide developed by phage display for targeting gastric cancer

A CD44 specific peptide developed by phage display for targeting gastric cancer

Gastric cancer stem cells: evidence, potential markers, and clinical implications

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

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